Current Position:Home>>Kits>>ELISA Kits>> Mouse ANP(Atrial Natriuretic Peptide) ELISA Kit

Mouse ANP(Atrial Natriuretic Peptide) ELISA Kit

Citations(5)Uniprot : P05125

Cat.No.:E-EL-M0166
Reactivity: Mouse

Filter:

ELISA

To Purchase E-EL-M0166

Size:

96T
48T
24T
96T*5
96T*10

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes ANP in samples. No significant cross-reactivity or interference between ANP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of ANP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ANP. During the reaction, ANP in the sample or standard competes with a fixed amount of ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ANP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ANP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ANP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	47.50	157.20	342.30	43.00	163.10	362.80
Standard deviation	3.00	7.70	13.30	2.60	7.20	12.70
CV (%)	6.32	4.90	3.89	6.05	4.41	3.50

Recovery

The recovery of ANP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-109	101
EDTA plasma(n=8)	93-105	98
Cell culture media(n=8)	84-99	91

Target Information

Database Links	SwissProt: P05125
Synonyms	NPPA, ANF, ANP, ATFB6, ATRST2, CDD, CDD-ANF, CDP, PND, Atrial natriuretic peptide, natriuretic peptide A
Research Area	Cancer, Cardiovascular, Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

ACTA PHARMACOLOGICA SINICA (2022) IF: 7.169
Porcupine inhibitor CGX1321 alleviates heart failure with preserved ejection fraction in mice by blocking WNT signaling

DOI: 10.1038/s41401-022-01025-y

Sample: serum
eLife (2018) IF: 7.616
Arterial smooth muscle cell PKD2 (TRPP1) channels regulate systemic blood pressure

DOI: 10.7554/eLife.42628

PMID: 30511640
Sample: Plasma
Journal of the American Heart Association (2021) IF: 5.501
Recombinant Soluble Corin Improves Cardiac Function in Mouse Models of Heart Failure

DOI: 10.1161/JAHA.120.019961

PMID: 33759549
Sample: Plasma
JOURNAL OF INFECTIOUS DISEASES (2015) IF: 5.997
Nonstructural Protein 1 (NS1)-Mediated Inhibition of c-Abl Results in Acute Lung Injury and Priming for Bacterial Co-infections: Insights Into 1918 H1N1 Pandemic?

DOI: 10.1093/infdis/jiu609

Sample: Serum
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY (2022) IF: 2.963
Knockout of Cardiac Troponin I-Interacting Kinase leads to cardiac dysfunction and remodeling

DOI: 10.1111/1440-1681.13699

Sample: serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha