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Mouse BNP(Brain Natriuretic Peptide) ELISA Kit

Citations(12)Uniprot : P40753

Cat.No.:E-EL-M0204
Reactivity: Mouse

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes BNP in samples. No significant cross-reactivity or interference between BNP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of BNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BNP. During the reaction, BNP in the sample or standard competes with a fixed amount of BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level BNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level BNP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	51.40	130.40	492.60	52.70	130.30	444.70
Standard deviation	3.20	7.20	22.70	2.70	7.80	17.30
CV (%)	6.23	5.52	4.61	5.12	5.99	3.89

Recovery

The recovery of BNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-100	95
EDTA plasma(n=8)	95-108	100
Cell culture media(n=8)	91-105	98

Linearity

Samples were spiked with high concentrations of Mouse BNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P40753
Synonyms	NPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B
Research Area	Cancer, Cardiovascular, Neuroscience

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

Acta Pharmaceutica Sinica B (2022) IF: 14.903
Compound Danshen Dripping Pill inhibits hypercholesterolemia/atherosclerosis-induced heart failure in ApoE and LDLR dual deficient mice via multiple mechanisms

DOI: 10.1016/j.apsb.2022.11.012

Sample: heart
ACTA PHARMACOLOGICA SINICA (2022) IF: 7.169
Porcupine inhibitor CGX1321 alleviates heart failure with preserved ejection fraction in mice by blocking WNT signaling

DOI: 10.1038/s41401-022-01025-y

Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
MCC950 attenuates doxorubicin-induced myocardial injury in vivo and in vitro by inhibiting NLRP3-mediated pyroptosis

DOI: 10.1016/j.biopha.2021.112133

PMID: 34474337
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity

DOI: 10.1016/j.biopha.2021.111531

PMID: 34311530
Sample: Serum
Pharmaceuticals (2022) IF: 5.215
Long-Term Sleep Deprivation-Induced Myocardial Remodeling and Mitochondrial Dysfunction in Mice Were Attenuated by Lipoic Acid and N-Acetylcysteine

DOI: 10.3390/ph16010051

Sample: serum
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022) IF: 5.31
LncRNA HOX transcript antisense RNA mitigates cardiac function injury in chronic heart failure via regulating microRNA-30a-5p to target KDM3A

DOI: 10.1111/jcmm.17160

PMID: 35083842
Sample: plasma
Journal of the American Heart Association (2021) IF: 5.501
Recombinant Soluble Corin Improves Cardiac Function in Mouse Models of Heart Failure

DOI: 10.1161/JAHA.120.019961

PMID: 33759549
Sample: Plasma
Journal of Translational Medicine (2021) IF: 5.531
microRNA-193-3p attenuates myocardial injury of mice with sepsis via STAT3/HMGB1 axis

DOI: 10.1186/s12967-021-03022-x

PMID: 34503521
Sample: serum
Frontiers in Cardiovascular Medicine (2022) IF: 5.846
Role of the Gut Microbiota in Glucose Metabolism During Heart Failure

DOI: 10.3389/fcvm.2022.903316

PMID: 35859583
Sample: Serum
Biomedicines (2022) IF: 4.757
Tetrahydrobiopterin (BH4) Supplementation Prevents the Cardiorenal Effects of Diabetes in Mice by Reducing Oxidative Stress, Inflammation and Fibrosis

DOI: 10.3390/biomedicines10102479

PMID: 36289741
Sample: plasma
JOURNAL OF ENDOCRINOLOGY (2014) IF: 3.586
PACAP is essential for the adaptive thermogenic response of brown adipose tissue to cold exposure.

DOI: 10.1530/joe-14-0316

PMID: 25056115
Sample: Plasma
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY (2022) IF: 2.963
Knockout of Cardiac Troponin I-Interacting Kinase leads to cardiac dysfunction and remodeling

DOI: 10.1111/1440-1681.13699

Sample: serum

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