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Rat IFN-γ(Interferon Gamma) ELISA Kit

Citations(36)Uniprot : P01581

  • Cat.No.:E-EL-R0009

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IFN-γ in samples. No significant cross-reactivity or interference between Rat IFN-γ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IFN-γ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IFN-γ. You can calculate the concentration of Rat IFN-γ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IFN-γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IFN-γ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 109.20 267.20 908.90 99.30 278.90 944.10
Standard deviation 6.00 14.70 48.20 6.10 16.50 34.00
CV (%) 5.49 5.50 5.30 6.14 5.92 3.60

Recovery

The recovery of Rat IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 84-97 91
EDTA plasma (n=8) 90-103 98
Cell culture media (n=8) 91-106 98

Linearity

Samples were spiked with high concentrations of Rat IFN-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P01581
SynonymsIFNG, IFG, IFI, Type II Interferon
Research AreaCancer, Immunology, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. CARDIOVASCULAR RESEARCH (2022) IF: 13.081
    Co-inhibition of immunoproteasome subunits LMP2 and LMP7 enables prevention of transplant arteriosclerosis

    DOI: 10.1093/cvr/cvac181
    Sample: CD4+ T cells
  2. Advanced Healthcare Materials (2022) IF: 11.092
    An Acellular Scaffold Facilitates Endometrial Regeneration and Fertility Restoration via Recruiting Endogenous Mesenchymal Stem Cells

    DOI: 10.1002/adhm.202201680

    PMID: 36049781

    		 Sample: Uterine
  3. Theranostics (2021) IF: 11.556
    Reducing PD-L1 expression with a self-assembled nanodrug: an alternative to PD-L1 antibody for enhanced chemo-immunotherapy

    DOI: 10.7150/thno.45777

    PMID: 33408792

    		 Sample: Tissue homogenate
  4. CELLULAR AND MOLECULAR LIFE SCIENCES (2016) IF: 9.2609
    toxin B

    DOI: 10.1007/s00018-016-2426-4
    Sample: Cell culture supernatant
  5. LIFE SCIENCES (2021) IF: 5.037
    Protective effects of hesperidin through attenuation of Ki67 expression against DMBA-induced breast cancer in female rats

    DOI: 10.1016/j.lfs.2021.119957

    PMID: 34530017

    		 Sample: Breast tissue
  6. Frontiers in Immunology (2020) IF: 5.085
    Corilagin Ameliorates Atherosclerosis in Peripheral Artery Disease via the Toll-Like Receptor-4 Signaling Pathway in vitro and in vivo

    DOI: 10.3389/fimmu.2020.01611
    Sample: plasma
  7. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Exploring the anti-inflammatory potential of Colocasia esculenta root extract in in-vitro and in-vivo models of inflammation

    DOI: 10.1016/j.jep.2022.116021
    Sample: serum
  8. PHYTOMEDICINE (2021) IF: 5.34
    Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa

    DOI: 10.1016/j.phymed.2021.153875

    PMID: 34911003

    		 Sample: serum
  9. JOURNAL OF CELLULAR PHYSIOLOGY (2017) IF: 4.08
    MSCs ameliorates DPN induced cellular pathology via [Ca2+]i homeostasis and scavenging the pro‐inflammatory cytokines

    DOI: 10.1002/jcp.26009
    Sample: neuronal cell
  10. ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.109
    hUC-MSCs exosomal miR-451 alleviated acute lung injury by modulating macrophage M2 polarization via regulating MIF-PI3K-AKT signaling pathway

    DOI: 10.1002/tox.23639
    Sample: serum,NR8383 cell
  11. ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119
    LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

    DOI: 10.1002/tox.23464

    PMID: 35006638

    		 Sample: bronchoalveolar lavage fluid (BALF)
  12. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY (2022) IF: 4.369
    BMSC-EVs overexpressing PD-L1 ameliorate DSS-induced ulcerative colitis in rats by regulating Th17/Treg cell balance through PTEN/PI3K/AKT/mTOR axis

    DOI: 10.1111/jgh.15987
    Sample: blood,colon
  13. PSYCHOPHARMACOLOGY (2021) IF: 4.53
    Effects of catechin on a rodent model of autism spectrum disorder: implications for the role of nitric oxide in neuroinflammatory pathway

    DOI: 10.1007/s00213-021-05941-5

    PMID: 34448020

    		 Sample: brain
  14. INFLAMMATION (2022) IF: 4.657
    Hederagenin Suppresses Inflammation and Cartilage Degradation to Ameliorate the Progression of Osteoarthritis: An In vivo and In vitro Study

    DOI: 10.1007/s10753-022-01763-5
    Sample: knee articular tissue
  15. Scientific Reports (2022) IF: 4.996
    Immunomodulatory activity of extracts from five edible basidiomycetes mushrooms in Wistar albino rats

    DOI: 10.1038/s41598-022-16349-2

    PMID: 35859110

    		 Sample: serum
  16. Food Bioscience (2020) IF: 3.067
    Curcumin combined with Baicalin attenuated ethanol-induced hepatitis by suppressing p38 MAPK and TSC1/ eIF-2α/ATF4 pathways in rats

    DOI: 10.1016/j.fbio.2020.100851
    Sample: liver tissue lysate
  17. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2019) IF: 3.091
    Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

    DOI: 10.1111/aji.13096
    Sample: Serum
  18. MOLECULES (2018) IF: 3.098
    Immunosuppressive Effect of Geniposide on Mitogen-Activated Protein Kinase Signalling Pathway and Their Cross-Talk in Fibroblast-Like Synoviocytes of Adjuvant Arthritis Rats

    DOI: 10.3390/molecules23010091
    Sample: fibroblast-like synoviocytes (FLS)
  19. REPRODUCTIVE TOXICOLOGY (2022) IF: 3.143
    Toxicity from a single injection of human umbilical cord mesenchymal stem cells into rat ovaries

    DOI: 10.1016/j.reprotox.2022.03.006

    PMID: 35307492

    		 Sample: serum
  20. NEUROSCIENCE LETTERS (2022) IF: 3.197
    Anti-depressant effects of acupuncture: The insights from NLRP3 mediated pyroptosis and inflammation

    DOI: 10.1016/j.neulet.2022.136787

    PMID: 35820551

    		 Sample: serum
  21. BMC NEUROSCIENCE (2021) IF: 3.288
    Metformin attenuates sepsis-induced neuronal injury and cognitive impairment

    DOI: 10.1186/s12868-021-00683-8

    PMID: 34911449

    		 Sample: hippocampus tissue
  22. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity

    DOI: 10.1016/j.biopha.2018.07.081
    Sample: Serum
  23. NEUROSCIENCE (2018) IF: 3.59
    Inhibiting Succinate Dehydrogenase by Dimethyl Malonate Alleviates Brain Damage in a Rat Model of Cardiac Arrest

    DOI: 10.1016/j.neuroscience.2018.09.041

    PMID: 30300703

    		 Sample: Serum
  24. BMC IMMUNOLOGY (2022) IF: 3.594
    NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis

    DOI: 10.1186/s12865-022-00515-2

    PMID: 35965334

    		 Sample: serum,muscle
  25. JOURNAL OF CELLULAR PHYSIOLOGY (2018) IF: 3.923
    Dehydroepiandrosterone stimulates inflammation and impairs ovarian functions of polycystic ovary syndrome

    DOI: 10.1002/jcp.27501

    PMID: 30580448

    		 Sample: Serum
  26. APOPTOSIS (2018) IF: 3.967
    MiRNA-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis

    DOI: 10.1007/s10495-018-1474-7
    Sample: Serum
  27. INTERNATIONAL JOURNAL OF NEUROSCIENCE (2020) IF: 2.107
    The dynamic expression of canonical Wnt/β-catenin signalling pathway in the pathologic process of experimental autoimmune neuritis

    DOI: 10.1080/00207454.2020.1725511
    Sample: sciatic nerves
  28. Computational and Mathematical Methods in Medicine (2022) IF: 2.238
    Influence of Melatonin on Behavioral and Neurological Function of Rats with Focal Cerebral Ischemia-Reperfusion Injury via the JNK/FoxO3a/Bim Pathway

    DOI: 10.1155/2022/8202975

    PMID: 35082916

    		 Sample: hippocampal tissue
  29. TOXICOLOGY MECHANISMS AND METHODS (2019) IF: 2.276
    Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats

    DOI: 10.1080/15376516.2018.1542474
    Sample: cardiac tissues,renal tissues
  30. Toxicology Research (2020) IF: 2.283
    The effects of phenanthrene exposure on Treg and Th17 cells related cytokines in female rats

    DOI: 10.1093/toxres/tfaa030

    PMID: 32670559

    		 Sample: Tissue homogenate
  31. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Accelerated lipid peroxidation in a rat model of gentamicin nephrotoxicity

    DOI: 10.3892/etm.2021.10652

    PMID: 34584563

    		 Sample: serum
  32. Dose-Response (2022) IF: 2.623
    Pharmacological Role of Capparis decidua (Forssk.) Edgew in Preventing Cyclophosphamide-induced Myelosuppression and Modulating Innate and Adaptive Immune Response:

    DOI: 10.1177/15593258221123672
    Sample: serum
  33. VIRUS RESEARCH (2014) IF: 2.827
    Galectin-9 ameliorates respiratory syncytial virus-induced pulmonary immunopathology through regulating the balance between Th17 and regulatory T cells

    DOI: 10.1016/j.virusres.2014.10.011
    Sample: Tissue homogenate
  34. JOURNAL OF CELLULAR BIOCHEMISTRY (2018) IF: 2.959
    Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix

    DOI: 10.1002/jcb.28243

    PMID: 30520122

    		 Sample: Serum,Bronchoalveolar Lavage Fluid
  35. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Macleaya cordata extracts exert antiviral effects in newborn mice with rotavirus‑induced diarrhea via inhibiting the JAK2/STAT3 signaling pathway

    DOI: 10.3892/etm.2020.8766
    Sample: Feces
  36. Molecular Medicine Reports (2018) IF: 1.922
    Sparstolonin B attenuates spinal cord injury‑induced inflammation in rats by modulating TLR4‑trafficking

    DOI: 10.3892/mmr.2018.8561
    Sample: spinal cord
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