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Rat IL-1α(Interleukin 1 Alpha) ELISA Kit

Citations(13)Uniprot : P16598

Cat.No.:E-EL-R0011
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat IL-1α in samples. No significant cross-reactivity or interference between Rat IL-1α and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat IL-1α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1α. You can calculate the concentration of Rat IL-1α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-1α were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	45.60	159.90	473.30	45.40	160.00	489.20
Standard deviation	2.40	7.50	22.70	2.40	8.80	20.50
CV (%)	5.26	4.69	4.80	5.29	5.50	4.19

Recovery

The recovery of Rat IL-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-100	94
EDTA plasma (n=8)	86-99	92
Cell culture media (n=8)	94-108	101

Linearity

Samples were spiked with high concentrations of Rat IL-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P16598
Synonyms	IL1A, IL1-A, IL1, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha
Research Area	Cancer, Metabolism, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Journal of Neuroinflammation (2020) IF: 8.3219
Microglia induce the transformation of A1/A2 reactive astrocytes via the CXCR7/PI3K/Akt pathway in chronic post-surgical pain

DOI: 10.1186/s12974-020-01891-5

PMID: 32665021
Sample: Tissue homogenate
JOURNAL OF NEUROCHEMISTRY (2021) IF: 5.372
Zinc induces reactive astrogliosis through ERK dependent activation of Stat3 and promotes synaptic degeneration

DOI: 10.1111/jnc.15531

Sample: primary astrocyte
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Role of Glutamine-Glutamate/GABA cycle and potential target GLUD2 in alleviation of rheumatoid arthritis by Tripterygium hypoglaucum (levl.) Hutch based on metabolomics and molecular pharmacology

DOI: 10.1016/j.jep.2021.114561

PMID: 34454056
Sample: serum
CHEMOSPHERE (2018) IF: 4.427
Fluoride altered rat's blood testis barrier by affecting the F-actin via IL-1α

DOI: 10.1016/j.chemosphere.2018.08.009

Sample: Tissue homogenate
NEUROBIOLOGY OF AGING (2018) IF: 4.454
CIP2A-promoted astrogliosis induces AD-like synaptic degeneration and cognitive deficits

DOI: 10.1016/j.neurobiolaging.2018.11.023

PMID: 30594047
Sample: Tissue homogenate,Cell culture medium
MEDIATORS OF INFLAMMATION (2020) IF: 4.711
Exploring the Mechanism on the Medullary Visceral Zone Inhibiting the Cholinergic Anti-inflammatory Pathway Induced by Sepsis

DOI: 10.1155/2020/1320278

PMID: 33061821
Sample: Serum
EXPERIMENTAL EYE RESEARCH (2022) IF: 3.467
A modified high-yield method for primary culture of rat retinal microglial cells

DOI: 10.1016/j.exer.2021.108919

PMID: 34979098
Sample: microglial cell
ORAL DISEASES (2021) IF: 3.511
Pilocarpine improves submandibular gland dysfunction in irradiated rats by downregulating the tight junction protein claudin-4

DOI: 10.1111/odi.13870

Sample: glands
Diagnostics (2022) IF: 3.706
Combination Antiretroviral Therapy (cART) in Diabetes Exacerbates Diabetogenic Effects on Hippocampal Microstructure, Neurogenesis and Cytokine Perturbation in Male Sprague Dawley Rats

DOI: 10.3390/diagnostics12040905

PMID: 35453953
Sample: brain
INTERNATIONAL IMMUNOPHARMACOLOGY (2014) IF: 2.711
Anti-inflammatory effects and pharmacokinetics study of geniposide on rats with adjuvant arthritis

DOI: 10.1016/j.intimp.2014.11.017

Sample: Cell culture supernatant
Iranian Journal of Basic Medical Sciences (2018) IF: 1.514
The protective effects of naringin against 5-fluorouracil-induced hepatotoxicity and nephrotoxicity in rats

DOI: 10.22038/IJBMS.2018.27510.6714

Sample: Serum
International Journal of Endocrinology (2015) IF: 1.948
17β Estradiol Modulates Perfusion Pressure and Expression of 5-LOX and CYP450 4A in the Isolated Kidney of Metabolic Syndrome Female Rats

DOI: 10.1155/2015/149408

Sample: Tissue homogenate
Toxicology Reports (2023)
Interactions of alcohol and combination antiretroviral (cART) drug in diabetic male Sprague Dawley rats: Hippocampal perturbations and toxicosis

DOI: 10.1016/j.toxrep.2023.01.009

Sample: brain

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