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Rat IL-4(Interleukin 4) ELISA Kit

Citations(29)Uniprot : P20096

  • Cat.No.:E-EL-R0014

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IL-4 in samples. No significant cross-reactivity or interference between Rat IL-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IL-4 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-4. You can calculate the concentration of Rat IL-4 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-4 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 47.20 134.60 394.80 47.80 134.90 433.70
Standard deviation 3.20 6.50 15.40 2.50 5.90 21.30
CV (%) 6.78 4.83 3.90 5.23 4.37 4.91

Recovery

The recovery of Rat IL-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 97
EDTA plasma (n=8) 86-99 93
Cell culture media (n=8) 91-103 96

Linearity

Samples were spiked with high concentrations of Rat IL-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P20096
SynonymsIL4, BCGF-1, BCGF1, BSF-1, BSF1
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Advanced Healthcare Materials (2022) IF: 11.092
    An Acellular Scaffold Facilitates Endometrial Regeneration and Fertility Restoration via Recruiting Endogenous Mesenchymal Stem Cells

    DOI: 10.1002/adhm.202201680

    PMID: 36049781

    		 Sample: Uterine
  2. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
    A novel hierarchical biofunctionalized 3D-printed porous Ti6Al4V scaffold with enhanced osteoporotic osseointegration through osteoimmunomodulation

    DOI: 10.1186/s12951-022-01277-0

    PMID: 35123501

    		 Sample: rat bone marrow mesenchymal stem cells (rBMSCs)
  3. JOURNAL OF PINEAL RESEARCH (2014) IF: 7.812
    Melatonin normalizes clinical and biochemical parameters of mild inflammation in diet‐induced metabolic syndrome in rats

    DOI: 10.1111/jpi.12168
    Sample: Plasma
  4. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Exploring the anti-inflammatory potential of Colocasia esculenta root extract in in-vitro and in-vivo models of inflammation

    DOI: 10.1016/j.jep.2022.116021
    Sample: serum
  5. Frontiers in Cellular and Infection Microbiology (2022) IF: 5.293
    Intestinal Epithelial Cell Exosome Launches IL-1β-Mediated Neuron Injury in Sepsis-Associated Encephalopathy

    DOI: 10.3389/fcimb.2021.783049

    PMID: 35111693

    		 Sample: serum,hippocampal tissue
  6. PHYTOMEDICINE (2021) IF: 5.34
    Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa

    DOI: 10.1016/j.phymed.2021.153875

    PMID: 34911003

    		 Sample: serum
  7. JOURNAL OF CELLULAR PHYSIOLOGY (2017) IF: 4.08
    MSCs ameliorates DPN induced cellular pathology via [Ca2+]i homeostasis and scavenging the pro‐inflammatory cytokines

    DOI: 10.1002/jcp.26009
    Sample: neuronal cell
  8. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Role of Glutamine-Glutamate/GABA cycle and potential target GLUD2 in alleviation of rheumatoid arthritis by Tripterygium hypoglaucum (levl.) Hutch based on metabolomics and molecular pharmacology

    DOI: 10.1016/j.jep.2021.114561

    PMID: 34454056

    		 Sample: serum
  9. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY (2022) IF: 4.369
    BMSC-EVs overexpressing PD-L1 ameliorate DSS-induced ulcerative colitis in rats by regulating Th17/Treg cell balance through PTEN/PI3K/AKT/mTOR axis

    DOI: 10.1111/jgh.15987
    Sample: blood,colon
  10. INFLAMMOPHARMACOLOGY (2021) IF: 4.473
    Exploring the role of Aquaporins (AQPs) in LPS induced systemic inflammation and the ameliorative effect of Garcinia in male Wistar rat

    DOI: 10.1007/s10787-021-00832-9

    PMID: 34106384

    		 Sample: serum
  11. MEDIATORS OF INFLAMMATION (2020) IF: 4.711
    Exposure to Air Pollution Exacerbates Inflammation in Rats with Preexisting COPD

    DOI: 10.1155/2020/4260204

    PMID: 32454790

    		 Sample: Tissue homogenate,Bronchoalveolar Lavage Fluid
  12. MOLECULES (2022) IF: 4.927
    The Pharmacokinetics in Mice and Cell Uptake of Thymus Immunosuppressive Pentapeptide Using LC-MS/MS Analysis

    DOI: 10.3390/molecules27134256

    PMID: 35807500

    		 Sample: RBL-2H3 cell
  13. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    The effect of myocardial infarction-associated transcript 2 (Mirt2) and miR-101 on sepsis-induced myocardial injury in rats.

    DOI: 10.26355/eurrev_202006_21528
    Sample: Serum
  14. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2019) IF: 3.091
    Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

    DOI: 10.1111/aji.13096
    Sample: Serum
  15. PHYTOTHERAPY RESEARCH (2017) IF: 3.092
    Antiinflammation Effects and Mechanisms Study of Geniposide on Rats with Collagen‐Induced Arthritis

    DOI: 10.1002/ptr.5775
    Sample: Cell culture supernatant
  16. MOLECULES (2018) IF: 3.098
    Immunosuppressive Effect of Geniposide on Mitogen-Activated Protein Kinase Signalling Pathway and Their Cross-Talk in Fibroblast-Like Synoviocytes of Adjuvant Arthritis Rats

    DOI: 10.3390/molecules23010091
    Sample: fibroblast-like synoviocytes (FLS)
  17. REPRODUCTIVE TOXICOLOGY (2022) IF: 3.143
    Toxicity from a single injection of human umbilical cord mesenchymal stem cells into rat ovaries

    DOI: 10.1016/j.reprotox.2022.03.006

    PMID: 35307492

    		 Sample: serum
  18. Biomed Research International (2021) IF: 3.411
    Celastrol Attenuates Learning and Memory Deficits in an Alzheimer’s Disease Rat Model

    DOI: 10.1155/2021/5574207
    Sample: brain
  19. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity

    DOI: 10.1016/j.biopha.2018.07.081
    Sample: Serum
  20. NEUROSCIENCE (2022) IF: 3.59
    High Environmental Temperature: Insights into Behavioural, Neurodevelopmental and Gut Microbiome Changes Following Gestational Exposure in Rats

    DOI: 10.1016/j.neuroscience.2022.02.026

    PMID: 35231581

    		 Sample: Brain
  21. Frontiers in Pharmacology (2018) IF: 3.831
    Anti-inflammatory Mechanism of Geniposide: Inhibiting the Hyperpermeability of Fibroblast-Like Synoviocytes via the RhoA/p38MAPK/NF-κB/F-Actin Signal Pathway

    DOI: 10.3389/fphar.2018.00105
    Sample: fibroblast-like synoviocytes (FLSs)
  22. INTERNATIONAL JOURNAL OF NEUROSCIENCE (2020) IF: 2.107
    The dynamic expression of canonical Wnt/β-catenin signalling pathway in the pathologic process of experimental autoimmune neuritis

    DOI: 10.1080/00207454.2020.1725511
    Sample: sciatic nerves
  23. Iranian Journal of Basic Medical Sciences (2020) IF: 2.146
    Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain

    DOI: 10.22038/ijbms.2020.45193.10527

    PMID: 33149866

    		 Sample: Tissue homogenate
  24. BMC Complementary and Alternative Medicine (2019) IF: 2.479
    In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds

    DOI: 10.1186/s12906-019-2776-1
    Sample: RBL-2H3 cell
  25. INTERNATIONAL IMMUNOPHARMACOLOGY (2014) IF: 2.711
    Effects and mechanisms of Geniposide on rats with adjuvant arthritis

    DOI: 10.1016/j.intimp.2014.02.021
    Sample: Cell lysate
  26. JOURNAL OF CELLULAR BIOCHEMISTRY (2018) IF: 2.959
    Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix

    DOI: 10.1002/jcb.28243

    PMID: 30520122

    		 Sample: Serum,Bronchoalveolar Lavage Fluid
  27. CHEMICAL & PHARMACEUTICAL BULLETIN (2018) IF: 1.258
    Effect of the Tibetan Medicine Zuotai on Degranulation and Inflammatory Mediator Release in RBL-2H3 Cells

    DOI: 10.1248/cpb.c18-00014
    Sample: Cells
  28. International Journal of Endocrinology (2015) IF: 1.948
    17β Estradiol Modulates Perfusion Pressure and Expression of 5-LOX and CYP450 4A in the Isolated Kidney of Metabolic Syndrome Female Rats

    DOI: 10.1155/2015/149408
    Sample: Tissue homogenate
  29. Toxicology Research (2017) IF: 1.969
    Nano NiO induced liver toxicity via activating the NF-κB signaling pathway in rats

    DOI: 10.1039/C6TX00444J
    Sample: Tissue homogenate
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