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HIS(Histamine) ELISA Kit

Citations(30)

  • Cat.No.:E-EL-0032

  • Reactivity: Universal
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes HIS in samples. No significant cross-reactivity or interference between HIS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of HIS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with HIS. During the reaction, HIS in the sample or standard competes with a fixed amount of HIS on the solid phase supporter for sites on the Biotinylated Detection Ab specific to HIS. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HIS in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level HIS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level HIS were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 5.17 13.32 45.10 5.30 13.54 42.52
Standard deviation 0.27 0.72 1.51 0.28 0.61 1.68
CV (%) 5.22 5.41 3.35 5.28 4.51 3.95

Recovery

The recovery of HIS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-100 95
EDTA plasma(n=8) 87-100 93
Cell culture media(n=8) 89-105 97

Linearity

Samples were spiked with high concentrations of HIS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaImmunology

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
elisa assay procedure 2

2. Aspirate and wash the plate for 3 times
elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 5

5. Add 50μL Stop Solution
elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Frontiers in Immunology (2022) IF: 8.786
    Nano-silica particles synergistically IgE-mediated mast cell activation exacerbating allergic inflammation in mice

    DOI: 10.3389/fimmu.2022.911300

    PMID: 35936002

    		 Sample: bronchoalveolar lavage fluid (BALF),serum
  2. FOOD CHEMISTRY (2021) IF: 7.514
    A new method to reduce allergenicity by improving the functional properties of soybean 7S protein through covalent modification with polyphenols

    DOI: 10.1016/j.foodchem.2021.131589
    Sample: plasma,KU812 cell
  3. Frontiers in Immunology (2021) IF: 7.561
    ORMDL3 Functions as a Negative Regulator of Antigen-Mediated Mast Cell Activation via an ATF6-UPR-Autophagy–Dependent Pathway

    DOI: 10.3389/fimmu.2021.604974
    Sample: Cell（MC/9）
  4. Science China-Life Sciences (2021) IF: 6.038
    Fullerene nanoparticles for the treatment of ulcerative colitis

    DOI: 10.1007/s11427-021-2001-0

    PMID: 34735681

    		 Sample: serum
  5. Food & Function (2022) IF: 6.317
    Hypoallergenic derivatives of Scylla paramamosain heat-stable allergens alleviated food allergy symptoms in Balb/c mice

    DOI: 10.1039/D2FO02184F

    PMID: 36318047

    		 Sample: serum
  6. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Propofol pretreatment alleviates mast cell degranulation by inhibiting SOC to protect the myocardium from ischemia–reperfusion injury

    DOI: 10.1016/j.biopha.2022.113014
    Sample: serum,RBL-2H3 cell
  7. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022) IF: 5.279
    Untargeted Metabolomic Profiling Reveals Changes in Gut Microbiota and Mechanisms of Its Regulation of Allergy in OVA-Sensitive BALB/c Mice

    DOI: 10.1021/acs.jafc.1c07482

    PMID: 35232013

    		 Sample: Serum
  8. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021) IF: 5.279
    Reducing the Allergenicity of α-Lactalbumin after Lipid Peroxidation

    DOI: 10.1021/acs.jafc.1c00559

    PMID: 33974424

    		 Sample: Cell culture supernatant
  9. iScience (2021) IF: 5.458
    Strong Histamine Torsion Raman Spectrum Enables Direct, Rapid and Ultrasensitive Detection of Allergic Diseases

    DOI: 10.1016/j.isci.2021.103384
    Sample: serum
  10. Foods (2023) IF: 5.561
    Establishment of Food Allergy Model in Dextran Sulfate Sodium Induced Colitis Mice

    DOI: 10.3390/foods12051007
    Sample: Serum
  11. Nutrients (2021) IF: 5.719
    Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

    DOI: 10.3390/nu13093209

    PMID: 34579088

    		 Sample: serum
  12. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice

    DOI: 10.3390/ijms23010226

    PMID: 35008651

    		 Sample: serum
  13. LWT-FOOD SCIENCE AND TECHNOLOGY (2020) IF: 4.006
    Screening of probiotic Lactobacilli with potential anti-allergic activity based on hyaluronidase inhibition and degranulation of RBL-2H3 cells in vitro

    DOI: 10.1016/j.lwt.2020.110707
    Sample: Cell culture supernatant
  14. Parasites & Vectors (2022) IF: 4.047
    Oral immunization of mice with recombinant Lactobacillus plantarum expressing a Trichinella spiralis galectin induces an immune protection against larval challenge

    DOI: 10.1186/s13071-022-05597-w
  15. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Biochemical analysis reveals the systematic response of motion sickness mice to ginger (Zingiber officinale) extract's amelioration effect

    DOI: 10.1016/j.jep.2022.115077

    PMID: 35131339

    		 Sample: blood
  16. MOLECULES (2021) IF: 4.412
    Mechanistic Studies of the Antiallergic Activity of Phyllanthus amarus Schum. & Thonn. and Its Compounds

    DOI: 10.3390/molecules26030695
    Sample: Cell culture supernatant
  17. MOLECULES (2021) IF: 4.412
    Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 Cells

    DOI: 10.3390/molecules26113298

    PMID: 34070943

    		 Sample: Cell culture supernatant
  18. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    		 Sample: Serum,Cell culture supernatant
  19. PLoS Neglected Tropical Diseases (2022) IF: 4.781
    Oral vaccination of mice with attenuated Salmonella encoding Trichinella spiralis calreticulin and serine protease 1.1 confers protective immunity in BALB/c mice

    DOI: 10.1371/journal.pntd.0010929
    Sample: intestinal tissue
  20. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2020) IF: 3.098
    Sini San ameliorates duodenal mucosal barrier injury and low‑grade inflammation via the CRF pathway in a rat model of functional dyspepsia

    DOI: 10.3892/ijmm.2019.4394
    Sample: Tissue homogenate
  21. MOLECULES (2018) IF: 3.098
    Coptisine Suppresses Mast Cell Degranulation and Ovalbumin-Induced Allergic Rhinitis

    DOI: 10.3390/molecules23113039

    PMID: 30469322

    		 Sample: Cell culture supernatant
  22. DRUG AND CHEMICAL TOXICOLOGY (2021) IF: 3.356
    Rupatadine protects the intestinal mucosa from injury by 5-flurouracil via modulation of inflammation, apoptosis and intestinal permeability

    DOI: 10.1080/01480545.2021.1997541

    PMID: 34747284

    		 Sample: intestinal
  23. Frontiers in Pharmacology (2019) IF: 3.845
    Protective Effects of Live Combined B. subtilis and E. faecium in Polymicrobial Sepsis Through Modulating Activation and Transformation of Macrophages and Mast Cells

    DOI: 10.3389/fphar.2018.01506

    PMID: 30719003

    		 Sample: Serum
  24. BMC Complementary and Alternative Medicine (2019) IF: 2.479
    In vitro anti-allergic activity of Moringa oleifera Lam. extracts and their isolated compounds

    DOI: 10.1186/s12906-019-2776-1
    Sample: RBL-2H3 cell
  25. BIOLOGICAL RESEARCH (2019) IF: 2.482
    Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice

    DOI: 10.1186/s40659-019-0216-9

    PMID: 30823938

    		 Sample: Plasma
  26. BIOSCIENCE REPORTS (2019) IF: 2.535
    Accurately cleavable goat β-lactoglobulin signal peptide efficiently guided translation of a recombinant human plasminogen activator in transgenic rabbit mammary gland

    DOI: 10.1042/BSR20190596
    Sample: plasma
  27. MEDICAL SCIENCE MONITOR (2021) IF: 2.649
    Human Umbilical Cord Mesenchymal Stem Cell Therapy Mitigates Interstitial Cystitis by Inhibiting Mast Cells

    DOI: 10.12659/MSM.930001

    PMID: 34354037

    		 Sample: bladder tissue,RBL-2H3 cell
  28. FEBS Open Bio (2021) IF: 2.693
    Salvinorin A inhibits ovalbumin-stimulated allergic rhinitis and RBL-2H3 cells degranulation

    DOI: 10.1002/2211-5463.13219

    PMID: 34092045

    		 Sample: RBL-2H3 cells
  29. FEMS MICROBIOLOGY LETTERS (2021) IF: 2.742
    Recombination Lactococcus lactis expressing Helicobacter pylori neutrophil-activating protein A attenuates food allergy symptoms in mice

    DOI: 10.1093/femsle/fnab034
    Sample: serum
  30. Experimental and Therapeutic Medicine (2016) IF: 1.28
    Anti‑allergic effect of Artemisia extract in rats

    DOI: 10.3892/etm.2016.3361
    Sample: Cell culture supernatant
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