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GSH(Glutathione) ELISA Kit

Cat.No.:E-EL-0026
Reactivity: Universal

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	1.56-100 μg/mL
Sensitivity	0.94 μg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes GSH in samples. No significant cross-reactivity or interference between GSH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of GSH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GSH. During the reaction, GSH in the sample or standard competes with a fixed amount of GSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GSH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GSH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GSH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GSH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (μg/mL)	5.06	13.81	43.81	5.19	13.60	42.40
Standard deviation	0.27	0.82	2.24	0.27	0.66	1.59
CV (%)	5.34	5.94	5.11	5.20	4.85	3.74

Recovery

The recovery of GSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-101	94
EDTA plasma(n=8)	88-101	96
Cell culture media(n=8)	86-99	93

Target Information

Research Area

Signal Transduction, Cell Biology, Metabolism,

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

Journal of Advanced Research (2022) IF: 10.479
Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria

DOI: 10.1016/j.jare.2022.05.010

PMID: 35640876
Sample: leaf
FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
Inhibition of ferroptosis attenuates oligospermia in male Nrf2 knockout mice

DOI: 10.1016/j.freeradbiomed.2022.10.314

PMID: 36309297
Sample: seminal plasma
Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
Downregulation of SIRT3 Aggravates Lung Ischemia Reperfusion Injury by Increasing Mitochondrial Fission and Oxidative Stress through HIF-1α-Dependent Mechanisms

DOI: 10.1155/2022/9041914

Sample: lung tissue
ARCHIVES OF TOXICOLOGY (2023) IF: 6.168
The circadian rhythm gene Bmal1 ameliorates acute deoxynivalenol-induced liver damage

DOI: 10.1007/s00204-022-03431-x

Sample: liver,serum
Antioxidants (2021) IF: 6.313
Ameliorative Effects of the Sesquiterpenoid Valerenic Acid on Oxidative Stress Induced in HepG2 Cells after Exposure to the Fungicide Benomyl

DOI: 10.3390/antiox10050746

PMID: 34066673
Sample: Cell lysate
BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
Pentoxifylline and berberine mitigate diclofenac-induced acute nephrotoxicity in male rats via modulation of inflammation and oxidative stress

DOI: 10.1016/j.biopha.2022.113225

PMID: 35671584
Sample: serum
Nutrients (2022) IF: 6.706
Puerariae lobatae Radix Alleviates Pre-Eclampsia by Remodeling Gut Microbiota and Protecting the Gut and Placental Barriers

DOI: 10.3390/nu14235025

Sample: serum,placental
Biology-Basel (2021) IF: 5.079
Cellular Stress Pathways Are Linked to Acetamiprid-Induced Apoptosis in SH-SY5Y Neural Cells

DOI: 10.3390/biology10090820

Sample: SH-SY5Y cell
Marine Drugs (2022) IF: 5.118
Anti-Photoaging Effect of Hydrolysates from Pacific Whiting Skin via MAPK/AP-1, NF-κB, TGF-β/Smad, and Nrf-2/HO-1 Signaling Pathway in UVB-Induced Human Dermal Fibroblasts

DOI: 10.3390/md20050308

Sample: Human dermal fibroblasts (HDFs)
Pharmaceuticals (2022) IF: 5.215
Long-Term Sleep Deprivation-Induced Myocardial Remodeling and Mitochondrial Dysfunction in Mice Were Attenuated by Lipoic Acid and N-Acetylcysteine

DOI: 10.3390/ph16010051

Sample: serum
FREE RADICAL BIOLOGY AND MEDICINE (2019) IF: 5.657
Protective effect of Prunella vulgaris var. L extract against blue light induced damages in ARPE-19 cells and mouse retina

DOI: 10.1016/j.freeradbiomed.2019.12.003

Sample: ARPE-19 cell
INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
rmMANF prevents sepsis-associated lung injury via inhibiting endoplasmic reticulum stress-induced ferroptosis in mice

DOI: 10.1016/j.intimp.2022.109608

Sample: lung tissues
Nutrients (2021) IF: 5.719
Effects of 12 Weeks Cosmos caudatus Supplement among Older Adults with Mild Cognitive Impairment: A Randomized, Double-Blind and Placebo-Controlled Trial

DOI: 10.3390/nu13020434

PMID: 33572715
Sample: Serum
Frontiers in Pharmacology (2021) IF: 5.811
The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

DOI: 10.3389/fphar.2021.709702

Sample: serum
Drug Design Development and Therapy (2021) IF: 4.162
Role of Oxidative Stress and Reduced Endogenous Hydrogen Sulfide in Diabetic Nephropathy

DOI: 10.2147/DDDT.S291591

PMID: 33707940
Sample: Tissue homogenate
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
Centella asiatica extract protects against cisplatin-induced hepatotoxicity via targeting oxidative stress, inflammation, and apoptosis

DOI: 10.1007/s11356-022-18626-z

PMID: 35029831
Sample: hepatic tissue
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
Biochemical investigation of human exposure to aflatoxin M1 and its association with risk factors of diabetes mellitus

DOI: 10.1007/s11356-021-14871-w

PMID: 34216342
Sample: serum
ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022) IF: 4.478
Blood serum lipid profiling may improve the management of recurrent miscarriage: a combination of machine learning of mid-infrared spectra and biochemical assays

DOI: 10.1007/s00216-022-04370-3

PMID: 36227296
Sample: blood
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2021) IF: 3
Limonin modulated immune and inflammatory responses to suppress colorectal adenocarcinoma in mice model

DOI: 10.1007/s00210-021-02101-6

PMID: 34009457
Sample: Serum
NEUROSCIENCE LETTERS (2021) IF: 3.046
Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

DOI: 10.1016/j.neulet.2021.136385

PMID: 34871743
Sample: hippocampus,liver
EUROPEAN JOURNAL OF NEUROSCIENCE (2020) IF: 3.115
Neuroprotective effects of brain‐derived neurotrophic factor against amyloid beta 1‐40‐induced retinal and optic nerve damage

DOI: 10.1111/ejn.14662

Sample: Tissue homogenate
Journal of Functional Foods (2017) IF: 3.144
Antioxidant and DNA protective effects of NTX, a proprietary glycyrrhizin/d-mannitol product, in association with alcohol consumption: A randomized, placebo-controlled, double-blind, crossover study

DOI: 10.1016/j.jff.2017.04.011

Sample: testes
Journal of Cardiovascular Translational Research (2022) IF: 3.216
Therapeutic Effects of Salvianolic Acid B on Angiotensin II–Induced Atrial Fibrosis by Regulating Atrium Metabolism via Targeting AMPK/FoxO1/miR-148a-3p Axis

DOI: 10.1007/s12265-022-10303-3

Sample: myofibroblasts (MFBs)
INFLAMMOPHARMACOLOGY (2020) IF: 3.238
Perindopril ameliorates experimental Alzheimer’s disease progression: role of amyloid β degradation, central estrogen receptor and hyperlipidemic-lipid raft signaling

DOI: 10.1007/s10787-020-00724-4

Sample: Serum,Tissue homogenate
PLoS One (2021) IF: 3.24
A low direct electrical signal attenuates oxidative stress and inflammation in septic rats

DOI: 10.1371/journal.pone.0257177

PMID: 34499695
Sample: serum,Lung tissue
METABOLIC BRAIN DISEASE (2022) IF: 3.584
Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

DOI: 10.1007/s11011-022-00952-3

PMID: 35298730
Sample: hippocampal,hepatic
JOURNAL OF APPLIED TOXICOLOGY (2022) IF: 3.628
Protective effect of pterostilbene in a streptozotocin-induced mouse model of Alzheimer's disease by targeting monoamine oxidase B

DOI: 10.1002/jat.4355

PMID: 35665945
Sample: hippocampus tissue
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2022) IF: 3.652
Zoledronic acid-induced oxidative damage and endoplasmic reticulum stress-mediated apoptosis in human embryonic kidney (HEK-293) cells

DOI: 10.1002/jbt.23083

PMID: 35587103
Sample: HEK-293 cell
Scientific Reports (2020) IF: 3.998
Reproductive effects of subchronic exposure to acetamiprid in male rats

DOI: 10.1038/s41598-020-65887-0

PMID: 32488017
Sample: Plasma,Tissue homogenate
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05
Metformin attenuated histopathological ocular deteriorations in a streptozotocin-induced hyperglycemic rat model

DOI: 10.1007/s00210-020-01989-w

PMID: 33047165
Sample: Serum
MOLECULAR VISION (2017) IF: 2.057
Pinus densiflora bark extract prevents selenite-induced cataract formation in the lens of Sprague Dawley rat pups

PMID: 28943754
Sample: lens tissue
MOLECULAR VISION (2019) IF: 2.174
Polyphenol-enriched fraction of Vaccinium uliginosum L. protects selenite-induced cataract formation in the lens of Sprague-Dawley rat pups

PMID: 30820147
Sample: lens tissue
Journal of Natural Medicines (2021) IF: 2.343
Dehydroabietic acid improves nonalcoholic fatty liver disease through activating the Keap1/Nrf2-ARE signaling pathway to reduce ferroptosis

DOI: 10.1007/s11418-021-01491-4

PMID: 33590347
Sample: Tissue homogenate(liver)
NEUROCHEMICAL RESEARCH (2017) IF: 2.581
Antioxidative and Anti-Apoptotic Roles of Silibinin in Reversing Learning and Memory Deficits in APP/PS1 Mice

DOI: 10.1007/s11064-017-2389-3

Sample: Tissue homogenate
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

DOI: 10.1155/2022/7832540

Sample: hepatic tissue
Dose-Response (2022) IF: 2.658
Pharmacological Evaluation of Mentha piperita Against Urolithiasis: An In Vitro and In Vivo Study:

DOI: 10.1177/15593258211073087

Sample: kidney
Gastroenterology Report (2020) IF: 2.96
Effect of Helicobacter pylori-eradication therapy on hepatic steatosis in patients with non-alcoholic fatty liver disease: a randomized–controlled pilot study

DOI: 10.1093/gastro/goz058

Sample: Serum
TOXICOLOGY AND INDUSTRIAL HEALTH (2019) IF: 1.635
Toxic effects of subchronic oral acetamiprid exposure in rats:

DOI: 10.1177/0748233719893203

Sample: plasma,renal,liver
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH (2021) IF: 1.671
Effects of shikonin on the development of ovarian follicles and female germline stem cells:

DOI: 10.1177/03000605211029461

Sample: blood
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS (2022) IF: 1.753
Protective effect by low-intensity downhill running training against muscle damage and oxidative stress after high-intensity downhill running in rats

DOI: 10.1590/0001-3765202220200265

PMID: 35507975
Sample: Plasma,vastus intermedius muscle tissue
Molecular Medicine Reports (2018) IF: 1.922
Embelin inhibits abdominal aortic aneurysm through decreasing IL‑6‑induced STAT3 and NF‑κB inactivation

DOI: 10.3892/mmr.2018.9221

Sample: Aortic tissues
ANDROLOGIA (2020) IF: 1.951
Androgenic effect of aqueous leaf extract of Moringa oleifera on Leydig TM3 cells in vitro

DOI: 10.1111/and.13825

PMID: 32926461
Sample: Cell lysate
Journal of Taibah University Medical Sciences (2022)
Effect of histone deacetylase inhibitor (vorinostat) on new-onset diabetes induced by tacrolimus

DOI: 10.1016/j.jtumed.2022.07.004

PMID: 36398015
Sample: blood

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Reviews/Q&A

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Reviews
Q&A

S********uSubmitted [ Feb 14 2024 ]

Asked: I would like to ask how to caculate the Absolute OD from Average OD? I get the Average OD value from machine, but your standard curve is made of Absolute OD, right?

adminSubmitted [ Feb 14 2024 ]

Answered: Dear Qu, Thanks for your enquiry, the Absolute OD value is obtained by subtracting the blank value from the Average OD value； For your enquired reference E-EL-0026 GSH(Glutathione) ELISA Kit, it follows to a competitive detection principle, the standard curve is Average OD and no need to subtracting the blank value； There are some tips for calculating the ELISA sample data: (It is recommended to use 4-parameter calculation. The software named OriginPro is recommended. You could get the software from the Internet https://www.originlab.com. (The software offers a free 7-day trial). How to use OriginPro to calculate the results is shown on our website: https://www.elabscience.com/List-detail-198.html . If you have difficulties calculating the results or is inconvenient to download the software, you could send the raw data to us, and we will help calculate and analyze.） Best Regards,

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DOI: 10.1016/j.jare.2022.05.010
PMID: 35640876	Sample: leaf

DOI: 10.1016/j.freeradbiomed.2022.10.314
PMID: 36309297	Sample: seminal plasma

DOI: 10.3390/antiox10050746
PMID: 34066673	Sample: Cell lysate

DOI: 10.1016/j.biopha.2022.113225
PMID: 35671584	Sample: serum

DOI: 10.3390/md20050308
	Sample: Human dermal fibroblasts (HDFs)

DOI: 10.1016/j.freeradbiomed.2019.12.003
	Sample: ARPE-19 cell

DOI: 10.1016/j.intimp.2022.109608
	Sample: lung tissues

DOI: 10.3390/nu13020434
PMID: 33572715	Sample: Serum

DOI: 10.2147/DDDT.S291591
PMID: 33707940	Sample: Tissue homogenate

DOI: 10.1007/s11356-022-18626-z
PMID: 35029831	Sample: hepatic tissue

DOI: 10.1007/s00204-022-03431-x
	Sample: liver,serum

DOI: 10.3390/nu14235025
	Sample: serum,placental

DOI: 10.1007/s00216-022-04370-3
PMID: 36227296	Sample: blood

DOI: 10.1016/j.neulet.2021.136385
PMID: 34871743	Sample: hippocampus,liver

DOI: 10.1007/s11356-021-14871-w
PMID: 34216342	Sample: serum

DOI: 10.1007/s00210-021-02101-6
PMID: 34009457	Sample: Serum

DOI: 10.1007/s12265-022-10303-3
	Sample: myofibroblasts (MFBs)

DOI: 10.1007/s10787-020-00724-4
	Sample: Serum,Tissue homogenate

DOI: 10.1371/journal.pone.0257177
PMID: 34499695	Sample: serum,Lung tissue

DOI: 10.1007/s11011-022-00952-3
PMID: 35298730	Sample: hippocampal,hepatic

DOI: 10.1002/jat.4355
PMID: 35665945	Sample: hippocampus tissue

DOI: 10.1002/jbt.23083
PMID: 35587103	Sample: HEK-293 cell

DOI: 10.1038/s41598-020-65887-0
PMID: 32488017	Sample: Plasma,Tissue homogenate

DOI: 10.1007/s00210-020-01989-w
PMID: 33047165	Sample: Serum

GSH(Glutathione) ELISA Kit

To Purchase E-EL-0026

Product Details

Properties

Test Principle

Kit components & Storage

Technical Data

Precision

Recovery

Target Information

Assay Procedures

Citations

Journal of Advanced Research (2022) IF: 10.479

FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010

Oxidative Medicine and Cellular Longevity (2022) IF: 7.31

ARCHIVES OF TOXICOLOGY (2023) IF: 6.168

Antioxidants (2021) IF: 6.313

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53

Nutrients (2022) IF: 6.706

Biology-Basel (2021) IF: 5.079

Marine Drugs (2022) IF: 5.118

Pharmaceuticals (2022) IF: 5.215

FREE RADICAL BIOLOGY AND MEDICINE (2019) IF: 5.657

INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714

Nutrients (2021) IF: 5.719

Frontiers in Pharmacology (2021) IF: 5.811

Drug Design Development and Therapy (2021) IF: 4.162

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022) IF: 4.478

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2021) IF: 3

NEUROSCIENCE LETTERS (2021) IF: 3.046

EUROPEAN JOURNAL OF NEUROSCIENCE (2020) IF: 3.115

Journal of Functional Foods (2017) IF: 3.144

Journal of Cardiovascular Translational Research (2022) IF: 3.216

INFLAMMOPHARMACOLOGY (2020) IF: 3.238

PLoS One (2021) IF: 3.24

METABOLIC BRAIN DISEASE (2022) IF: 3.584

JOURNAL OF APPLIED TOXICOLOGY (2022) IF: 3.628

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2022) IF: 3.652

Scientific Reports (2020) IF: 3.998

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05

MOLECULAR VISION (2017) IF: 2.057

MOLECULAR VISION (2019) IF: 2.174

Journal of Natural Medicines (2021) IF: 2.343

NEUROCHEMICAL RESEARCH (2017) IF: 2.581

Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65

Dose-Response (2022) IF: 2.658

Gastroenterology Report (2020) IF: 2.96

TOXICOLOGY AND INDUSTRIAL HEALTH (2019) IF: 1.635

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH (2021) IF: 1.671

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS (2022) IF: 1.753

Molecular Medicine Reports (2018) IF: 1.922

ANDROLOGIA (2020) IF: 1.951

Journal of Taibah University Medical Sciences (2022)

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DOI: 10.1007/s11418-021-01491-4
PMID: 33590347	Sample: Tissue homogenate(liver)

DOI: 10.1007/s11064-017-2389-3
	Sample: Tissue homogenate

DOI: 10.1177/0748233719893203
	Sample: plasma,renal,liver

DOI: 10.1590/0001-3765202220200265
PMID: 35507975	Sample: Plasma,vastus intermedius muscle tissue

DOI: 10.1111/and.13825
PMID: 32926461	Sample: Cell lysate

DOI: 10.1016/j.jtumed.2022.07.004
PMID: 36398015	Sample: blood