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Human ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit

Citations(13)Uniprot : Q9NQ30

Cat.No.:E-EL-H1557
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human ESM1 in samples. No significant cross-reactivity or interference between Human ESM1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human ESM1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ESM1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ESM1. You can calculate the concentration of Human ESM1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ESM1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ESM1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	54.84	154.68	423.76	52.25	168.48	438.78
Standard deviation	3.49	8.80	13.01	3.18	10.08	22.20
CV (%)	6.36	5.69	3.07	6.09	5.98	5.06

Recovery

The recovery of Human ESM1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	95-108	100
EDTA plasma (n=8)	86-101	92
Cell culture media (n=8)	92-105	98

Linearity

Samples were spiked with high concentrations of Human ESM1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q9NQ30
Synonyms	endocan,Endothelial cell-specific molecule 1
Research Area	Tags and Cell Markers

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF PERIODONTOLOGY (2017) IF: 3.03
Effect of Non‐Surgical Periodontal Treatment on Gingival Crevicular Fluid and Serum Endocan, Vascular Endothelial Growth Factor‐A, and Tumor Necrosis Factor‐Alpha Levels

DOI: 10.1902/jop.2016.160279

Sample: gingival crevicular fluid (GCF),serum
ANGIOLOGY (2021) IF: 3.619
Increased Carotid Intima–Media Thickness and Endothelial Cell-Specific Molecule-1 (Endocan) Levels in Obese Children:

DOI: 10.1177/0003319721995664

Sample: Serum
ANGIOLOGY (2021) IF: 3.619
Endocan: A Novel Predictor of Endothelial Dysfunction in Silent Brain Infarction:

DOI: 10.1177/00033197211012135

Sample: Serum
Experimental and Therapeutic Medicine (2021) IF: 2.447
The vasodilatory mechanism of nitric oxide and hydrogen sulfide in the human mesenteric artery in patients with colorectal cancer

DOI: 10.3892/etm.2021.9646

PMID: 33500703
Sample: Serum
Reproductive Sciences (2018) IF: 2.548
Maternal Serum Endocan Concentration in Pregnancies Complicated by Intrauterine Growth Restriction:

DOI: 10.1177/1933719118773480

Sample: Serum
International Journal of Chronic Obstructive Pulmonary Disease (2018) IF: 2.917
Serum endocan levels in patients with stable COPD

DOI: 10.2147/COPD.S182731

Sample: serum
JOURNAL OF PERINATAL MEDICINE (2019) IF: 1.361
Maternal serum endocan concentrations are elevated in patients with preterm premature rupture of membranes

DOI: 10.1515/jpm-2018-0401

PMID: 30875331
Sample: Serum
Journal of Maternal-Fetal & Neonatal Medicine (2018) IF: 1.493
Serum endocan concentration and its correlation with severity of hypertensive disorders in pregnancy

DOI: 10.1080/14767058.2018.1548597

PMID: 30501553
Sample: Serum
Journal of Maternal-Fetal & Neonatal Medicine (2019) IF: 1.569
The utility of maternal serum endocan level to predict preterm delivery within seven days in patients with threatened preterm labor

DOI: 10.1080/14767058.2019.1649388

Sample: serum
MEDICINE (2022) IF: 1.817
The investigation of levels of endothelial cell-specific molecule, progranuline, clusterin, and human epididymis protein 4 in the differential diagnosis of malignant pleural effusions

DOI: 10.1097/MD.0000000000032471

Sample: pleural effusion (PE)
European Journal of Rheumatology (2018)
The relationship of serum endocan levels and anti-TNF-alpha therapy in patients with ankylosing spondylitis

DOI: 10.5152/eurjrheum.2017.17287

Sample: Serum
JOURNAL OF OBSTETRICS AND GYNAECOLOGY (2020) IF: 0.8070
Maternal serum endothelial cell-specific molecule-1 level and its correlation with severity of early-onset preeclampsia

DOI: 10.1080/01443615.2020.1819215

PMID: 33228435
Sample: serum
JOURNAL OF OBSTETRICS AND GYNAECOLOGY (2020) IF: 0.8070
Endothelial cell-specific-molecule-1 (endocan) levels in women with premature ovarian insufficiency: a prospective comparative study

DOI: 10.1080/01443615.2020.1789952

PMID: 32808833
Sample: Serum

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