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Human CALP(Calprotectin) ELISA Kit

Citations(7)Uniprot : P05109,P06702

Cat.No.:E-EL-H2357
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human CALP in samples. No significant cross-reactivity or interference between Human CALP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human CALP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CALP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CALP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CALP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CALP. You can calculate the concentration of Human CALP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CALP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CALP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.91	15.02	39.32	5.24	14.96	38.26
Standard deviation	0.29	0.83	1.54	0.28	0.72	1.82
CV (%)	5.91	5.53	3.92	5.34	4.81	4.76

Recovery

The recovery of Human CALP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-99	92
EDTA plasma (n=8)	88-99	93
Cell culture media (n=8)	90-102	95

Linearity

Samples were spiked with high concentrations of Human CALP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05109 P06702
Research Area	Signal transduction, Cell Biology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Journal of Fungi (2021) IF: 5.816
Chronic Pulmonary Aspergillosis: Disease Severity Using Image Analysis and Correlation with Systemic Proinflammation and Predictors of Clinical Outcome

DOI: 10.3390/jof7100842

Sample: peripheral blood
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY (2018) IF: 3.201
Faecal but not serum calprotectin levels look promising in predicting active disease in Behçet's syndrome patients with gastrointestinal involvement.

PMID: 30582504
Sample: stool
SEMINARS IN DIALYSIS (2022) IF: 3.455
Peritoneal calprotectin level in peritoneal dialysis patients

DOI: 10.1111/sdi.13082

PMID: 35439838
Sample: peritoneal dialysate drainage fluid
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022) IF: 3.657
Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?

DOI: 10.1089/jir.2022.0098

PMID: 36179036
Sample: fecal
CLINICA CHIMICA ACTA (2019) IF: 2.735
Hybrid panel of biomarkers can be useful in the diagnosis of pleural and peritoneal effusions

DOI: 10.1016/j.cca.2019.07.015

PMID: 31310745
Sample: Bronchoalveolar Lavage Fluid
JOURNAL OF LARYNGOLOGY AND OTOLOGY (2019) IF: 1.261
A novel prognostic biomarker for recurrent aphthous stomatitis: calprotectin

DOI: 10.1017/S0022215119001440

Sample: serum
MEDICINE (2022) IF: 1.817
Serum calprotectin levels as markers of inflammation, insulin resistance and hyperandrogenism in women with polycystic ovary syndrome

DOI: 10.1097/MD.0000000000032326

Sample: serum

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Reviews/Q&A

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Reviews
Q&A

A************iSubmitted [ Jan 25 2024 ]

Asked: Hello, can I use this test on stool samples?

adminSubmitted [ Jan 25 2024 ]

Answered: Yes, this ELISA kit can be used to test feces samples.

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