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Cat.No.:E-EL-0070
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Assay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 18.75 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes ALD in samples. No significant cross-reactivity or interference between ALD and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of ALD concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ALD were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ALD were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 51.80 | 89.60 | 476.90 | 46.80 | 94.20 | 479.40 |
Standard deviation | 2.70 | 4.30 | 20.00 | 3.20 | 5.70 | 23.00 |
CV (%) | 5.21 | 4.80 | 4.19 | 6.84 | 6.05 | 4.80 |
The recovery of ALD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 86-98 | 91 |
EDTA plasma(n=8) | 96-112 | 102 |
Cell culture media(n=8) | 90-102 | 96 |
Samples were spiked with high concentrations of ALD and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Research Area | Signal transduction |
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
PMID: 31327271 |
Sample: Serum |
PMID: 36232551 |
Sample: serum |
Sample: plasma |
PMID: 35624870 |
Sample: Serum,urine |
Sample: Serum |
PMID: 33932738 |
Sample: Plasma |
DOI: 10.1155/2020/2978340 |
|
PMID: 32655765 |
Sample: Plasma,Urine,Tissue homogenate |
PMID: 33759549 |
Sample: Plasma |
Sample: Tissue homogenate |
PMID: 32768965 |
Sample: Plasma,Tissue homogenate |
Sample: serum,urine |
Sample: plasma |
PMID: 30422674 |
Sample: Plasma |
Sample: plasma |
Sample: heart tissue |
DOI: 10.1155/2019/5960563 |
|
Sample: serum |
DOI: 10.1111/jphp.13168 |
|
Sample: Serum |
Sample: Plasma |
Sample: plasma |
PMID: 30235329 |
Sample: Serum |
Sample: Cell culture supernatant |
DOI: 10.1155/2018/7341242 |
|
Sample: plasma |
Sample: serum |
PMID: 29268635 |
Sample: Plasma |
PMID: 33841721 |
Sample: serum |
PMID: 30318954 |
Sample: Plasma |
Sample: Plasma,Serum,Tissue homogenate |