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Mouse IgA(Immunoglobulin A) ELISA Kit

Cat.No.:E-EL-M0690
Reactivity: Mouse

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48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	6.25-400 ng/mL
Sensitivity	3.75 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse IgA in samples. No significant cross-reactivity or interference between Mouse IgA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse IgA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgA. You can calculate the concentration of Mouse IgA in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IgA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IgA were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	20.95	52.87	156.46	20.01	53.52	164.75
Standard deviation	1.44	2.29	8.53	1.08	3.18	7.51
CV (%)	6.87	4.33	5.45	5.40	5.94	4.56

Recovery

The recovery of Mouse IgA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-106	99
EDTA plasma (n=8)	87-99	93
Cell culture media (n=8)	93-110	101

Linearity

Samples were spiked with high concentrations of Mouse IgA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	IgA
Research Area	Cancer, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

International Journal of Nanomedicine (2022) IF: 6.4
The Immune-Enhancing Properties of Hwanglyeonhaedok-Tang-Mediated Biosynthesized Gold Nanoparticles in Macrophages and Splenocytes

DOI: 10.2147/IJN.S338334

PMID: 35125869
Sample: RAW264.7 cell,splenocytes
Scientific Reports (2016) IF: 5.228
Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice

DOI: 10.1038/srep34079

Sample: Serum
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 4.125
Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarum LP90 against dextran sulfate sodium-induced colitis in mice

DOI: 10.1002/jsfa.12173

PMID: 36054505
Sample: serum,colon tissue
Food & Function (2020) IF: 4.171
Melatonin as an inducer of arecoline and their coordinated roles in anti-oxidative activity and immune responses

DOI: 10.1039/D0FO01841D

PMID: 32955540
Sample: Plasma
Foods (2022) IF: 4.35
Lactobacillus fermentum Stimulates Intestinal Secretion of Immunoglobulin A in an Individual-Specific Manner

DOI: 10.3390/foods11091229

PMID: 35563952
Sample: Duodenum,jejunum,ileum,colon,colon contents
Journal of Functional Foods (2022) IF: 4.451
Immune-enhancing efficacy of Curtobacterium proimmune K3 lysates isolated from Panax ginseng beverages in cyclophosphamide-induced immunosuppressed mice

DOI: 10.1016/j.jff.2022.105020

Sample: serum
Journal of Functional Foods (2020) IF: 3.701
Dietary supplementation of Bacillus sp. DU106 activates innate immunity and regulates intestinal microbiota in mice

DOI: 10.1016/j.jff.2020.104247

Sample: Serum
MICROBIAL PATHOGENESIS (2019) IF: 2.581
Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

DOI: 10.1016/j.micpath.2019.103557

Sample: serum

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