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Mouse MUC5AC(Mucin-5 subtype AC) ELISA Kit

Citations(5)Uniprot : E9PWB6

Cat.No.:E-EL-M0799
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse MUC5AC in samples. No significant cross-reactivity or interference between Mouse MUC5AC and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse MUC5AC concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MUC5AC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MUC5AC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MUC5AC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MUC5AC. You can calculate the concentration of Mouse MUC5AC in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse MUC5AC were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse MUC5AC were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.51	1.56	3.44	0.54	1.53	3.65
Standard deviation	0.03	0.06	0.11	0.03	0.09	0.17
CV (%)	5.88	3.85	3.20	5.56	5.88	4.66

Recovery

The recovery of Mouse MUC5AC spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-107	99
EDTA plasma (n=8)	87-101	94
Cell culture media (n=8)	95-112	102

Linearity

Samples were spiked with high concentrations of Mouse MUC5AC and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: E9PWB6
Synonyms	Mucin 5,Subtypes A And C,
Research Area	Cancer, Immunology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Home Dust Mites Promote MUC5AC Hyper-Expression by Modulating the sNASP/TRAF6 Axis in the Airway Epithelium

DOI: 10.3390/ijms23169405

PMID: 36012669
Sample: BALF,serum,lung tissue
RESPIRATORY RESEARCH (2022) IF: 5.631
MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model

DOI: 10.1186/s12931-021-01920-8

PMID: 35042537
Sample: bronchoalveolar lavage fluid (BALF)
MOLECULES (2022) IF: 4.412
Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo

DOI: 10.3390/molecules27123789

PMID: 35744915
Sample: serum
Biomed Research International (2023) IF: 3.246
CAVO Inhibits Airway Inflammation and ILC2s in OVA-Induced Murine Asthma Mice

DOI: 10.1155/2023/8783078

Sample: bronchoalveolar lavage fluid (BALF)
Frontiers in Pharmacology (2019) IF: 3.845
Suhuang Antitussive Capsules-Ameliorative Effects on LPS-Induced Sputum Obstruction in Mice Through Promoting HGF Secretion

DOI: 10.3389/fphar.2019.01422

Sample: Serum,Tissue homogenate

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