Current Position:Home>>Kits>>ELISA Kits>> Human ET-1(Endothelin 1) ELISA Kit

Human ET-1(Endothelin 1) ELISA Kit

Citations(14)Uniprot : P05305

Cat.No.:E-EL-H0064
Reactivity: Human

Filter:

ELISA

To Purchase E-EL-H0064

Size:

96T
48T
24T
96T*5
96T*10

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.25-80 pg/mL
Sensitivity	0.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human ET-1 in samples. No significant cross-reactivity or interference between Human ET-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human ET-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ET-1. You can calculate the concentration of Human ET-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ET-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ET-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	4.00	7.80	33.60	4.00	7.70	36.30
Standard deviation	0.30	0.40	1.40	0.20	0.30	1.40
CV (%)	7.50	5.13	4.17	5.00	3.90	3.86

Recovery

The recovery of Human ET-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-103	97
EDTA plasma (n=8)	89-104	96
Cell culture media (n=8)	86-100	92

Linearity

Samples were spiked with high concentrations of Human ET-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05305
Synonyms	EDN1, ET1, HDLCQ7, PPET1, Preproendothelin-1
Research Area	Cancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Antioxidants (2022) IF: 7.675
Natural Activators of Autophagy Increase Maximal Walking Distance and Reduce Oxidative Stress in Patients with Peripheral Artery Disease: A Pilot Study

DOI: 10.3390/antiox11091836

PMID: 36139910
Sample: serum
Nanoscale (2019) IF: 6.97
Atmospheric nanoparticles affect vascular function using a 3D human vascularized organotypic chip

DOI: 10.1039/C9NR03622A

Sample: microvessels cells
Frontiers in Pharmacology (2021) IF: 5.811
Sinapine Thiocyanate Ameliorates Vascular Endothelial Dysfunction in Hypertension by Inhibiting Activation of the NLRP3 Inflammasome

DOI: 10.3389/fphar.2020.620159

PMID: 33633569
Sample: Cell culture supernatant
Frontiers in Pharmacology (2021) IF: 5.811
Identification of the Antithrombotic Mechanism of Leonurine in Adrenalin Hydrochloride-Induced Thrombosis in Zebrafish via Regulating Oxidative Stress and Coagulation Cascade.

DOI: 10.3389/fphar.2021.742954
American Journal of Translational Research (2021) IF: 4.06
Minimally-invasive stone removal in urinary calculi patients during pregnancy: clinical therapeutic effects and low complications

PMID: 34540086
Sample: serum
PHARMACOGENOMICS (2019) IF: 2.265
Association of endothelin receptor type A rs5333 gene polymorphism with steroid response in Egyptian children with idiopathic nephrotic syndrome

DOI: 10.2217/pgs-2018-0175

PMID: 30672385
Sample: Plasma
International Journal of Medical Sciences (2020) IF: 2.523
Shorter Leukocyte Telomere Length coupled with lower expression of Telomerase Genes in patients with Essential Hypertension

DOI: 10.7150/ijms.48456

Sample: plasma
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
Isoflavones from Semen Sojae Preparatum Improve Atherosclerosis and Oxidative Stress by Modulating Nrf2 Signaling Pathway through Estrogen-Like Effects

DOI: 10.1155/2022/4242099

PMID: 35432565
Sample: serum,EA hy926 cell
Polish Archives of Internal Medicine-Polskie Archiwum Medycyny Wewnetrznej (2018) IF: 2.658
High‑density lipoprotein cholesterol levels and pulmonary artery vasoreactivity in patients with idiopathic pulmonary arterial hypertension

DOI: 10.20452/pamw.4306

Sample: plasma
JOURNAL OF NEPHROLOGY (2018) IF: 2.724
The association of endothelin-1 levels with renal survival in polycystic kidney disease patients

DOI: 10.1007/s40620-018-0514-2

Sample: Serum
Medicina-Lithuania (2022) IF: 2.948
Changes in Left Heart Geometry, Function, and Blood Serum Biomarkers in Patients with Obstructive Sleep Apnea after Treatment with Continuous Positive Airway Pressure

DOI: 10.3390/medicina58111511

PMID: 36363468
Sample: serum
Brazilian Journal of Cardiovascular Surgery (2020) IF: 1.053
Comparison between the Effects of Bretschneider’s HTK Solution and Cold Blood Cardioplegia on Systemic Endothelial Functions in Patients who Undergo Coronary Artery Bypass Surgery: a Prospective Randomized and Controlled Trial

DOI: 10.21470/1678-9741-2019-0327

Sample: Serum
Journal of Maternal-Fetal & Neonatal Medicine (2017) IF: 1.826
Serum endothelin-1 and placental alkaline phosphatase levels in placenta percreta and normal pregnancies

DOI: 10.1080/14767058.2017.1297412

Sample: Serum
MEDICAL SCIENCE MONITOR (2019) IF: 1.98
Protective Effects of Dexmedetomidine and Oxycodone in Patients Undergoing Limb Ischemia-Reperfusion

DOI: 10.12659/MSM.918261

PMID: 31782408
Sample: Serum,Plasma

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha