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Cat.No.:E-EL-H1347
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 0.31-20 ng/mL |
Sensitivity | 0.19 ng/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human OPN in samples. No significant cross-reactivity or interference between Human OPN and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human OPN concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human OPN were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human OPN were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 0.97 | 2.46 | 7.02 | 1.05 | 2.26 | 6.75 |
Standard deviation | 0.06 | 0.14 | 0.28 | 0.05 | 0.11 | 0.23 |
CV (%) | 6.19 | 5.69 | 3.99 | 4.76 | 4.87 | 3.41 |
The recovery of Human OPN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 88-100 | 95 |
EDTA plasma (n=8) | 97-111 | 102 |
Cell culture media (n=8) | 85-95 | 90 |
Samples were spiked with high concentrations of Human OPN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P10451
|
Synonyms | SPP1, BNSP, BSP1, ETA-1, Bone Sialoprotein I, Secreted Phosphoprotein 1, Early T-Lymphocyte Activation 1 |
Research Area | Cancer, Cardiovascular, Signal transduction, Stem cells |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
Sample: plasma |
Sample: Serum |
Sample: plasma |
Sample: serum |
Sample: Serum |
S***********rSubmitted [ Feb 11 2024 ]
Asked: How many samples can you analyze with this kit?
Reply
adminSubmitted [ Feb 11 2024 ]
Answered: To ensure the accuracy of experimental results, we recommend to do duplicate for each standard and sample, detailed information i'll reply you by mail.
A*******hSubmitted [ Jan 27 2024 ]
Asked: How many samples can be tested in one Osteopontin Elisa Kit
Reply
adminSubmitted [ Jan 27 2024 ]
Answered: Hello Arya Shah,we will reply you by email later.