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Cat.No.:E-EL-H0110
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Assay type | Sandwich-ELISA |
Format | 96T |
Assay time | 3.5h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 18.75 pg/mL |
Sample type &Sample volume | Serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human TGF-β1 in samples. No significant cross-reactivity or interference between Human TGF-β1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human TGF-β1 concentrations in Serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TGF-β1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TGF-β1 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 106.20 | 233.50 | 976.30 | 109.60 | 234.10 | 999.00 |
Standard deviation | 7.20 | 11.90 | 51.70 | 5.60 | 12.40 | 43.00 |
CV (%) | 6.78 | 5.10 | 5.30 | 5.11 | 5.30 | 4.30 |
The recovery of Human TGF-β1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 94-105 | 100 |
EDTA plasma (n=8) | 90-104 | 96 |
Cell culture media (n=8) | 87-100 | 92 |
Database Links | SwissProt:
P01137
|
Synonyms | TGFB1, CED, DPD1, LAP, TGFB, TGFbeta, transforming growth factor beta 1 |
Research Area | Cancer,Cardiovascular,Signaling transduction,Stem cells |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
DOI: 10.7150/thno.31461 |
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Sample: platelet poor plasma (PPP) |
DOI: 10.1111/cpr.13316 |
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PMID: 35869570 |
Sample: HDFs |
DOI: 10.3390/biom13010073 |
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Sample: serum |
DOI: 10.1039/D2FO00477A |
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Sample: serum |
DOI: 10.1155/2022/5249367 |
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PMID: 35422896 |
Sample: Tregs |
DOI: 10.1002/JPER.20-0713 |
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PMID: 33382101 |
Sample: gingival crevicular fluid (GCF) |
Sample: anterior lens capsules (ALCs),HLE B-3 cells |
DOI: 10.1002/jcp.29713 |
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PMID: 32385929 |
Sample: Cell culture supernatant |
Sample: Normal human dermal fibroblasts (NHDF) |
DOI: 10.3390/nu13113770 |
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PMID: 34836025 |
Sample: serum |
PMID: 35821438 |
Sample: HMCs |
Sample: Cell culture medium |
DOI: 10.1002/tox.23464 |
|
PMID: 35006638 |
Sample: A549 cell |
PMID: 32681864 |
Sample: Serum |
PMID: 34237073 |
Sample: pleural fluid |
DOI: 10.1111/jcmm.14313 |
|
Sample: Serum |
PMID: 33159018 |
Sample: Serum |
Sample: MH7A cells |
DOI: 10.2147/DMSO.S319863 |
|
PMID: 34345175 |
Sample: serum |
PMID: 35980503 |
Sample: THP-1 cell,H1299 cell |
DOI: 10.1039/C9RA01031A |
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Sample: macrophages |
Sample: aortic tissue |
DOI: 10.1111/odi.14275 |
|
PMID: 35699366 |
Sample: plasma |
DOI: 10.1002/cbin.11806 |
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PMID: 35349767 |
Sample: HepG2 cell |
Sample: Cell culture supernatant |
DOI: 10.3233/JAD-200885 |
|
Sample: serum |
PMID: 35894403 |
Sample: blood |
PMID: 33421993 |
Sample: Plasma |
PMID: 33599951 |
Sample: Tissue(uterosacral ligament) |
DOI: 10.1111/ijcp.14728 |
|
Sample: serum |
PMID: 30632005 |
Sample: Cell culture medium |
DOI: 10.3390/app112110395 |
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Sample: dental pulp lysates |
DOI: 10.3390/app12031554 |
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Sample: Dental pulp tissue |
DOI: 10.3967/bes2022.089 |
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PMID: 35945185 |
Sample: THP-1 cell |
Sample: Cell culture supernatant |
Sample: serum |
DOI: 10.1002/jcla.23530 |
|
Sample: Serum |
PMID: 33080678 |
Sample: Cell culture supernatant |
Sample: Serum |
Sample: serum |