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Human hs-CRP(high-sensitivity C-Reactive Protein) ELISA Kit

Citations(24)Uniprot : P02741

  • Cat.No.:E-EL-H5134

  • Reactivity: Human
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To Purchase E-EL-H5134

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human hs-CRP in samples. No significant cross-reactivity or interference between Human hs-CRP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human hs-CRP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human hs-CRP. You can calculate the concentration of Human hs-CRP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human hs-CRP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human hs-CRP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 48.52 154.68 451.79 45.99 149.25 455.20
Standard deviation 3.24 7.55 24.26 2.99 8.42 19.71
CV (%) 6.68 4.88 5.37 6.50 5.64 4.33

Recovery

The recovery of Human hs-CRP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 83-97 90
EDTA plasma (n=8) 89-99 94
Cell culture media (n=8) 94-106 99

Linearity

Samples were spiked with high concentrations of Human hs-CRP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P02741
Research AreaCancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. JOURNAL OF INFECTIOUS DISEASES (2022) IF: 7.759
    The IDOze Study: The Link between Sleep Disruption and Tryptophan-Kynurenine Pathway Activation in Women with HIV

    DOI: 10.1093/infdis/jiac287
    Sample: plasma
  2. Frontiers in Nutrition (2022) IF: 6.59
    Different oral and gut microbial profiles in those with Alzheimer's disease consuming anti-inflammatory diets.

    DOI: 10.3389/fnut.2022.974694
    Sample: serum
  3. Sports Medicine-Open (2019) IF: 6.766
    The Effects of a Single Versus Three Consecutive Sessions of Football Training on Postprandial Lipemia: a Randomized, Controlled Trial in Healthy, Recreationally Active Males

    DOI: 10.1186/s40798-019-0212-1
    Sample: serum
  4. Journal of Functional Foods (2022) IF: 5.223
    High fiber multigrain supplementation improved disease activity score, circulating inflammatory and oxidative stress biomarkers in rheumatoid arthritis (RA) patients: A randomized human clinical trial

    DOI: 10.1016/j.jff.2022.105392
    Sample: blood
  5. Nutrients (2021) IF: 5.719
    Microbiota and Metabolite Modifications after Dietary Exclusion of Dairy Products and Reduced Consumption of Fermented Food in Young and Older Men

    DOI: 10.3390/nu13061905

    PMID: 34205926

    		 Sample: serum
  6. Nutrients (2018) IF: 4.196
    Inflammatory Dietary Pattern, IL-17F Genetic Variant, and the Risk of Colorectal Cancer

    DOI: 10.3390/nu10060724
    Sample: Plasma
  7. Nutrients (2020) IF: 4.546
    Supplementation with a Mango Leaf Extract (Zynamite®) in Combination with Quercetin Attenuates Muscle Damage and Pain and Accelerates Recovery after Strenuous Damaging Exercise

    DOI: 10.3390/nu12030614
    Sample: serum
  8. Clinical Epidemiology (2021) IF: 4.79
    Cardiovascular Disease Prediction by Machine Learning Algorithms Based on Cytokines in Kazakhs of China

    DOI: 10.2147/CLEP.S313343

    PMID: 34135637

    		 Sample: blood
  9. MEDIATORS OF INFLAMMATION (2019) IF: 3.545
    Nomogram Based on Cytokines for Cardiovascular Diseases in Xinjiang Kazakhs

    DOI: 10.1155/2019/4756295
    Sample: plasma
  10. HIV MEDICINE (2020) IF: 3.556
    Effects of antiretroviral therapy on proprotein convertase subtilisin/kexin 9: focus on lipids, inflammation and immunovirological parameters

    DOI: 10.1111/hiv.12884

    PMID: 32496664

    		 Sample: Plasma
  11. Journal of Cardiovascular Development and Disease (2022) IF: 3.948
    Discriminative Utility of Apelin-to-NT-Pro-Brain Natriuretic Peptide Ratio for Heart Failure with Preserved Ejection Fraction among Type 2 Diabetes Mellitus Patients

    DOI: 10.3390/jcdd9010023
    Sample: plasma
  12. PEDIATRIC PULMONOLOGY (2020) IF: 2.534
    Obstructive sleep apnea in children with hypothalamic obesity: Evaluation of possible related factors

    DOI: 10.1002/ppul.25097

    PMID: 32986303

    		 Sample: Plasma
  13. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Tamarindus indica Seed Extract-Based Botanical Compositions Alleviate Knee Pain and Improve Joint Function in Mild-to-Moderate Osteoarthritis: A Randomized, Double-Blind, Placebo-Controlled Clinical Study

    DOI: 10.1155/2022/2226139

    PMID: 35096104

    		 Sample: serum
  14. Dermatologic Therapy (2021) IF: 2.851
    Carotid intima-media thickness and serum proinflammatory cytokine levels in rosacea patients without cardiovascular risk factors

    DOI: 10.1111/dth.14733

    PMID: 33389789

    		 Sample: Serum
  15. JOURNAL OF THE NATIONAL MEDICAL ASSOCIATION (2020) IF: 1.03
    Cardiovascular Risk Indices and Their Impact on Outcome in Patients with Hyperglycaemic Emergencies in a Nigerian Hospital

    DOI: 10.1016/j.jnma.2019.12.004

    PMID: 31973880

    		 Sample: Serum
  16. Journal of BUON (2019) IF: 1.379
    Effects of postoperative enteral nutrition combined with adjuvant radiotherapy on inflammatory response, nutrition, healing and prognosis in patients receiving radical surgery for esophageal carcinoma.

    PMID: 31646824

    		 Sample: serum
  17. SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION (2020) IF: 1.475
    Association of serum FGF-23, klotho, fetuin-A, osteopontin, osteoprotegerin and hs-CRP levels with coronary artery disease

    DOI: 10.1080/00365513.2020.1728786
    Sample: serum
  18. JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
    Human clinical trial to assess the effect of consumption of multigrain Indian bread on glycemic regulation in type 2 diabetic participants

    DOI: 10.1111/jfbc.13465
    Sample: Plasma
  19. CLINICAL AND EXPERIMENTAL HYPERTENSION (2020) IF: 1.789
    The role of serum interleukin-37 levels, inflammation and blood pressure in patients with preeclampsia

    DOI: 10.1080/10641963.2020.1772813
    Sample: blood
  20. Therapeutics and Clinical Risk Management (2018) IF: 1.995
    Efficacy and safety of Ginkgo biloba extract as an “add-on” treatment to metformin for patients with metabolic syndrome: a pilot clinical study

    DOI: 10.2147/TCRM.S169503
    Sample: Serum
  21. Journal of Diabetes and Metabolic Disorders (2019)
    Subclinical inflammation and endothelial dysfunction are linked to cardiac autonomic neuropathy in type 2 diabetes

    DOI: 10.1007/s40200-019-00435-w
    Sample: blood
  22. Diabetes & Metabolic Syndrome-Clinical Research & Reviews (2020)
    Role of C-reactive Protein(CRP) or high-sensitivity CRP in predicting gestational diabetes Mellitus:Systematic review

    DOI: 10.1016/j.dsx.2020.02.004

    PMID: 32247209

    		 Sample: Serum
  23. Clinical Epidemiology and Global Health (2021)
    Predictor for cardiovascular risk in patients with type-2 diabetes mellitus

    DOI: 10.1016/j.cegh.2021.100870
    Sample: serum
  24. Cardiogenetics (2023)
    Lower Circulating Cell-Free Mitochondrial DNA Is Associated with Heart Failure in Type 2 Diabetes Mellitus Patients

    DOI: 10.3390/cardiogenetics13010003
    Sample: blood
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