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293 [HEK-293] Cell Line

Catalog number:EP-CL-0001

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1×10⁶ cells/vial

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Price: $302

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Origin and General Characteristics

Cell Name	293 [HEK-293] Cell Line
Age	Fetus
Tissue	Embryonic kidney
Morphology	Epithelial
Growth Properties	Adherent
Descriptions	Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome [RF32764], it is now clear that only left end sequences are present.The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19(19q13.2).
Biosafety Level	2

Culture Conditions and Handling

Complete Growth Medium	MEM +10% FBS +1% P/S
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio	1:4-1:8
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Special Features of the Cell Line

Tumorigenic	Yes
Effects	Yes, forms tumors in nude mice.
Receptor Expression	Vitronectin
Applications	Efficacy testing. Transfection host. Viruscide testing

Duration

The cell line does not adhere to the substrate when left at room temperature for any length of time, therefore, live cultures may be received with the cells detached.The cells will re-attach to the flask over a period of several days in culture at 37°C.