293 [HEK-293] Cell Line

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      Catalog number:EP-CL-0001

      Size:
      • 1×10⁶ cells/vial
      Qty:
      - +
      Price: $302

      Lead Time: 3-5 daysWelcome to order from local distributors.

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      Origin and General Characteristics

      Cell Name 293 [HEK-293] Cell Line
      Age Fetus
      Tissue Embryonic kidney
      Morphology Epithelial
      Growth Properties Adherent
      Descriptions Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome [RF32764], it is now clear that only left end sequences are present.The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19(19q13.2).
      Biosafety Level 2

      Culture Conditions and Handling

      Complete Growth Medium MEM +10% FBS +1% P/S
      Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
      Subcultivation Ratio 1:4-1:8
      Medium Renewal Every 2 to 3 days
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
      Storage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%
      Temperature: 37℃

      Special Features of the Cell Line

      Tumorigenic Yes
      Effects Yes, forms tumors in nude mice.
      Receptor Expression Vitronectin
      Applications Efficacy testing. Transfection host. Viruscide testing

      Duration

      The cell line does not adhere to the substrate when left at room temperature for any length of time, therefore, live cultures may be received with the cells detached.The cells will re-attach to the flask over a period of several days in culture at 37°C.

      Related Resources

      Recommendations for handling of cryopreserved cells

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