Current Position:Home>>Kits>>ELISA Kits>> 3-NT(3-Nitrotyrosine) ELISA Kit

3-NT(3-Nitrotyrosine) ELISA Kit

Cat.No.:E-EL-0040
Reactivity: Universal

Filter:

ELISA

To Purchase E-EL-0040

Size:

96T
48T
24T
96T*5
96T*10

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes 3-NT in samples. No significant cross-reactivity or interference between 3-NT and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of 3-NT concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 3-NT. During the reaction, 3-NT in the sample or standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 3-NT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 3-NT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 3-NT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 3-NT were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	5.00	12.50	38.30	5.10	13.10	36.60
Standard deviation	0.30	0.70	1.80	0.30	0.60	1.90
CV (%)	6.00	5.60	4.70	5.88	4.58	5.19

Recovery

The recovery of 3-NT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	97-108	102
EDTA plasma(n=8)	91-108	99
Cell culture media(n=8)	93-105	100

Linearity

Samples were spiked with high concentrations of 3-NT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	3-Nitro-L-Tyrosine, Nitrotyrosine
Research Area	Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

ENVIRONMENTAL POLLUTION (2022) IF: 9.988
Ambient fine particulate matter exposures and oxidative protein damage in early pregnant women

DOI: 10.1016/j.envpol.2022.120604

Sample: serum
Biomedicines (2021) IF: 6.081
Iron Replacement and Redox Balance in Non-Anemic and Mildly Anemic Iron Deficiency COPD Patients: Insights from a Clinical Trial

DOI: 10.3390/biomedicines9091191

PMID: 34572377
Sample: serum
Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
Protection against Doxorubicin-Induced Cardiotoxicity through Modulating iNOS/ARG 2 Balance by Electroacupuncture at PC6

DOI: 10.1155/2021/6628957

PMID: 33824696
Sample: Tissue homogenate
LIFE SCIENCES (2022) IF: 6.78
Attenuated effect of zinc gluconate on oxidative stress, inflammation, and angiogenic imbalance in pre-eclampsia rats

DOI: 10.1016/j.lfs.2022.121055

PMID: 36228770
Sample: serum,placental
Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression

DOI: 10.1155/2020/7106525

Sample: Tissue homogenate
Metabolites (2022) IF: 5.581
Curcumin and Vitamin C Attenuate Gentamicin-Induced Nephrotoxicity by Modulating Distinctive Reactive Species

DOI: 10.3390/metabo13010049

Sample: serum
Frontiers in Pharmacology (2021) IF: 5.811
Neuroprotection Against NMDA-Induced Retinal Damage by Philanthotoxin-343 Involves Reduced Nitrosative Stress

DOI: 10.3389/fphar.2021.798794

PMID: 34970151
Sample: retinal tissue
Scientific Reports (2021) IF: 4.38
Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study

DOI: 10.1038/s41598-021-91256-6

PMID: 34083710
Sample: saliva
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2019) IF: 3.559
Effect of a high sucrose and high fat diet in BDNF (+/-) mice on oxidative stress markers in adipose tissues

DOI: 10.1016/j.abb.2019.02.004

PMID: 30797748
Sample: Serum
MOLECULAR VISION (2018) IF: 2.219
Protective effect of magnesium acetyltaurate and taurine against NMDA-induced retinal damage involves reduced nitrosative stress

PMID: 30090013
Sample: retinal tissues
BIOLOGICAL TRACE ELEMENT RESEARCH (2018) IF: 2.361
Melatonin Ameliorates Neuropharmacological and Neurobiochemical Alterations Induced by Subchronic Exposure to Arsenic in Wistar Rats

DOI: 10.1007/s12011-018-1537-1

PMID: 30306420
Sample: Tissue homogenate
Experimental and Therapeutic Medicine (2021) IF: 2.447
Accelerated lipid peroxidation in a rat model of gentamicin nephrotoxicity

DOI: 10.3892/etm.2021.10652

PMID: 34584563
Sample: serum
PLoS One (2017) IF: 2.806
Reduction of oxidative-nitrosative stress underlies anticataract effect of topically applied tocotrienol in streptozotocin-induced diabetic rats

DOI: 10.1371/journal.pone.0174542

Sample: Tissue homogenate
Andrology (2020) IF: 2.86
Low androgen status inhibits erectile function by inducing eNOS uncoupling in rat corpus cavernosum

DOI: 10.1111/andr.12844

PMID: 32594586
Sample: Tissue homogenate
NEUROSCIENCE RESEARCH (2023) IF: 2.904
Neuroprotection by trans-resveratrol in rats with N-methyl-D-aspartate (NMDA)–induced retinal injury: insights into the role of adenosine A1 receptors

DOI: 10.1016/j.neures.2023.02.004

Sample: retinal
Journal of Pediatric Urology (2021) IF: 1.83
The effect of hypothermia in a rat testicular torsion/detorsion model

DOI: 10.1016/j.jpurol.2021.01.045

PMID: 33610458
Sample: Serum,Tissue homogenate(testis)

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha