Current Position:Home>>Kits>>ELISA Kits>> ADMA(Asymmetrical Dimethylarginine) ELISA Kit

ADMA(Asymmetrical Dimethylarginine) ELISA Kit

Cat.No.:E-EL-0042
Reactivity: Universal

Filter:

ELISA

To Purchase E-EL-0042

Size:

96T
48T
24T
96T*5
96T*10

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 ng/mL
Sensitivity	9.38 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes ADMA in samples. No significant cross-reactivity or interference between ADMA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of ADMA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ADMA. During the reaction, ADMA in the sample or standard competes with a fixed amount of ADMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ADMA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ADMA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ADMA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ADMA were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	53.20	120.80	371.60	50.50	121.30	371.70
Standard deviation	3.70	6.30	19.30	3.40	5.90	19.70
CV (%)	6.95	5.22	5.19	6.73	4.86	5.30

Recovery

The recovery of ADMA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-102	94
EDTA plasma(n=8)	96-111	102
Cell culture media(n=8)	94-108	101

Linearity

Samples were spiked with high concentrations of ADMA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	N, N-Dimethylarginine, NG-Dimethylarginine dihydrochloride, 2-Amino-5-[(amino-dimethylaminomethylene)amino]pentanoic acid
Research Area	Signal transduction, Cardiovascular

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

GeroScience (2021) IF: 7.713
Early manifestation of aging-related vascular dysfunction in human penile vasculature—A potential explanation for the role of erectile dysfunction as a harbinger of systemic vascular disease

DOI: 10.1007/s11357-021-00507-x

Sample: plasma
Antioxidants (2021) IF: 6.313
Urolithiasis Develops Endothelial Dysfunction as a Clinical Feature

DOI: 10.3390/antiox10050722

PMID: 34064366
Sample: Serum
Frontiers in Medicine (2022) IF: 5.058
Treatment with β-blocker nebivolol ameliorates oxidative stress and endothelial dysfunction in tenofovir-induced nephrotoxicity in rats.

DOI: 10.3389/fmed.2022.953749

Sample: plasma
Metabolites (2020) IF: 4.097
Posttranslational Modifications Pattern in Clear Cell Renal Cell Carcinoma

DOI: 10.3390/metabo11010010

PMID: 33375435
Sample: Serum
NITRIC OXIDE-BIOLOGY AND CHEMISTRY (2017) IF: 4.181
Relationship between asymmetric dimethylarginine, nitrite and genetic polymorphisms: Impact on erectile dysfunction therapy

DOI: 10.1016/j.niox.2017.10.006

Sample: Plasma
Journal of Clinical Medicine (2021) IF: 4.242
Cardiovascular Risk Factors and Their Relationship with Vascular Dysfunction in South African Children of African Ancestry

DOI: 10.3390/jcm10020354

PMID: 33477761
Sample: Urine
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
Dihydromyricetin increases endothelial nitric oxide production and inhibits atherosclerosis through microRNA‐21 in apolipoprotein E‐deficient mice

DOI: 10.1111/jcmm.15278

Sample: plasma
EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
Associations of inflammatory markers and vascular cell adhesion molecule-1 with endothelial dysfunction in collagen-induced arthritis

DOI: 10.1016/j.ejphar.2019.172786

PMID: 31712060
Sample: Serum
DIABETES-METABOLISM RESEARCH AND REVIEWS (2017) IF: 3.263
The association between dysregulated adipocytokines in early pregnancy and development of gestational diabetes

DOI: 10.1002/dmrr.2926

Sample: Serum
NITRIC OXIDE-BIOLOGY AND CHEMISTRY (2019) IF: 3.371
DDAH1 and DDAH2 polymorphisms associate with asymmetrical dimethylarginine plasma levels in erectile dysfunction patients but not in healthy controls

DOI: 10.1016/j.niox.2019.08.001

PMID: 31394201
Sample: Plasma
Obesity Research & Clinical Practice (2021) IF: 2.288
Fermented soybean enhances post-meal response in appetite-regulating hormones among Indonesian girls with obesity

DOI: 10.1016/j.orcp.2021.06.005

PMID: 34147377
Sample: plasma
Brazilian Journal of Cardiovascular Surgery (2020) IF: 1.053
Comparison between the Effects of Bretschneider’s HTK Solution and Cold Blood Cardioplegia on Systemic Endothelial Functions in Patients who Undergo Coronary Artery Bypass Surgery: a Prospective Randomized and Controlled Trial

DOI: 10.21470/1678-9741-2019-0327

Sample: Serum
JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION (2019) IF: 0.642
Comparison of asymmetric dimethylarginine levels between pre- and post-menopausal women - a cross-sectional study from Rawalpindi.

DOI: 10.5455/jpma.296507

Sample: Serum
African Health Sciences (2021) IF: 0.927
Gut permeability is associated with hypertension and measures of obesity but not with Endothelial Dysfunction in South African youth

DOI: 10.4314/ahs.v21i3.26

Sample: plasma

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha