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Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method)

Cat.No.:E-BC-K057-S
Detection instrument: Spectrophotometer(628 nm)

Detection method: Colorimetric method

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Detection principle

Bromocresol green (BCG) can combine with the albumin in pH 4.0~4.2 to form an albumin-BCG complex, which is yellowish-green. The depth of yellowish-green is proportional to the concentration of albumin. The serum albumin concentration can be calculated by measuring the OD value at 628 nm.

Performance characteristics

Synonyms	ALB
Sample type	Serum,plasma
Sensitivity	0.50 g/L
Detection range	0.50-70 g/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 4.200%Average intra-assay CV: 2.100%
Other instruments required	Micropipettor, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 1: 2-8℃, Reagent 2: -20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.50-70 g/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Mouse plasma	1
Rat serum	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

DOI: 10.3390/ijms232315400

Sample: plasma,urine
Drug Design Development and Therapy (2021) IF: 4.162
Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways

DOI: 10.2147/DDDT.S326975

PMID: 34703210
Sample: serum
International Journal of Environmental Research and Public Health (2022) IF: 3.39
Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners

DOI: 10.3390/ijerph19010498

PMID: 35010758
Sample: plasma
Journal of Oleo Science (2022) IF: 1.628
Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

DOI: 10.5650/jos.ess22033

PMID: 35965085
Sample: serum

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