Alternate Names	Integrin beta-1,ITB1,Itgb1,Fibronectin receptor subunit beta,VLA-4 subunit beta,CD29
Swissprot	P05556
References	1. Hemler M. 1990. Annu. Rev. Immunol. 8:365. 2. Hynes R. 1992. Cell 69:11.

Product Details

Form	Liquid
Clone No.	TS2/16.2.1(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	FITC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792C]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	FITC
	FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Human CD29 Antibody[TS2/16.2.1]	25μg, 100μg	Check More
Biotin Anti-Human CD29 Antibody[TS2/16.2.1]	25μg, 100μg	Check More
PE Anti-Human CD29 Antibody[TS2/16.2.1]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD29 Antibody[TS2/16.2.1]	20Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Human CD29 Antibody[TS2/16.2.1]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD29 Antibody[TS2/16.2.1]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD29 Antibody[TS2/16.2.1]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD29 Antibody[TS2/16.2.1]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD29 Antibody[TS2/16.2.1]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD29 Antibody[TS2/16.2.1]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD29 Antibody[TS2/16.2.1]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD29 Antibody[TS2/16.2.1]	20Tests, 100Tests, 100Tests×2	Check More

Citations

Nature Biomedical Engineering (2021) IF: 25.671
Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids

DOI: 10.1038/s41551-021-00774-1

PMID: 34341535
Sample: myoblasts

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