Alternate Names	CD81 antigen,CD81,26 kDa cell surface protein TAPA-1,Target of the antiproliferative antibody 1.Tetraspanin-28,Tspan-28,APA1, TSPAN28
Swissprot	P60033
References	1. Menno C, et al. 2010. J. Clin. Invest. 4:1265. 2. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127. 3. Wright M, et al. 1994. Immunol. Today 15:588.

Product Details

Form	Liquid
Clone No.	1.3.3.22(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	Elab Fluor^® 488 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792L]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 488
	Elab Fluor^® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expression on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8 and CD82 in T cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Human CD81 Antibody[1.3.3.22]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD81 Antibody[1.3.3.22]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD81 Antibody[1.3.3.22]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD81 Antibody[1.3.3.22]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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