Introduction
Endogenous
Peroxidase Blocking Buffer is mainly used to block endogenous peroxidase in
tissues or cells during immunohistochemistry (IHC), immunocytochemistry (ICC)
and in situ hybridization (ISH).
Endogenous
peroxidase is widely found in some cells and tissues, including red blood
cells, kidney and liver, which will result with a high background and even
false positive results when using peroxidase method to detect samples.
Therefore, cell or tissue samples should be blocked with appropriate peroxidase
blocking solution before staining to eliminate the interference of endogenous
peroxidase.
The
product is 3% H2O2, which is a ready-to-use buffer to
block the endogenous hydrogen peroxide of the sample without dilution.
Components
|
Cat.
|
Products
|
10 mL
|
20 mL
|
50 mL
|
Storage
|
|
E-IR-R115
|
Endogenous Peroxidase Blocking Buffer
|
10 mL
|
20 mL
|
50 mL
|
2~8°C
|
Experimental Procedure
1. For
IHC or ICC
1) Paraffin
slices need to be dewaxed, hydrated and then repaired. Cell slices and frozen slices
can neglect this step.
2) Suck
up the liquid on the surface of the slice, drop appropriate amount of Endogenous
Peroxidase Blocking Buffer to cover the sample completely, and incubate at RT
for 5~10 min or 37°C for 2~5 min.
3) Remove
the Endogenous Peroxidase Blocking Buffer, wash the sample with washing
solution or PBS and other appropriate solutions for 1~3 min/time, 2~3 times.
Then, follow the steps, such as blocking and primary antibody incubation.
2. For
ISH
1) Drop appropriate amount
of Endogenous Peroxidase Blocking Buffer to cover the sample completely, and
incubate at RT for 5~10 min or 37°C for 2~5 min.
2) Remove the blocking buffer,
wash with the washing solution of in situ hybridization for 2~5 min/time, 2~3
times. Then follow the steps such as hybridization.
Storage
Store
at 2~8°C. Valid for 12 months. The reagents are valid
within 6 months after opening.
Cautions
1. For some special cases where it
is difficult to use the peroxidase detection system, please try to use the
alkaline phosphatase detection system.
2. This product is for research
use only. It couldn’t be used for clinical diagnosis or treatment, food or
medicine, and can’t be stored in residence.
3. For your safety and health,
please wear the lab coat and disposable gloves before the experiments.
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