Excellent Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) substrate for horseradish peroxidase (HRP) enzyme that provides low picogram detection of proteins in Western blot and ELISA applications. Excellent Chemiluminescent Substrate emits light for longer and chemiluminescent signal at more than twice the intensity of other entry-level luminol-based horseradish peroxidase detection systems while also maintaining sensitivity and low background. In addition, the range of the calibration curve is much wider than other competitors. Also, the greater signal intensity is achieved using less primary and secondary antibody than typical ECL substrates. All of this for about 30% less cost than comparable products.

Method for use

ECL substrate -- An enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity of antibodies and other ELISA or Western blot probes.
Prepare Working Solution by mixing equal parts of the Substrate A and Substrate B. The Working Solution is stable for 8 hours at RT.
Please refer to the manual for the specific application method in the experiments.

Storage

2-8℃, 12 months

Citations

Molecular Metabolism (2021) IF: 7.422
Sodium-glucose co-transporter2 expression and inflammatory activity in diabetic atherosclerotic plaques: Effects of sodium-glucose co-transporter2 inhibitor treatment

DOI: 10.1016/j.molmet.2021.101337

PMID: 34500107
Antioxidants (2022) IF: 7.675
SIRT3 Modulates Endothelial Mitochondrial Redox State during Insulin Resistance

DOI: 10.3390/antiox11081611

PMID: 36009329
EUROPEAN JOURNAL OF CELL BIOLOGY (2023) IF: 6.02
Forskolin affects proliferation, migration and Paclitaxel-mediated cytotoxicity in Non-Small-Cell Lung Cancer cell lines via adenylyl cyclase/cAMP axis

DOI: 10.1016/j.ejcb.2023.151292
Biomedicines (2021) IF: 6.081
Metformin Therapy Effects on the Expression of Sodium-Glucose Cotransporter 2, Leptin, and SIRT6 Levels in Pericoronary Fat Excised from Pre-Diabetic Patients with Acute Myocardial Infarction

DOI: 10.3390/biomedicines9080904

PMID: 34440108
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Buffalo Milk Whey Activates Necroptosis and Apoptosis in a Xenograft Model of Colorectal Cancer

DOI: 10.3390/ijms23158464

PMID: 35955595
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Breed and Feeding System Impact the Bioactive Anti-Inflammatory Properties of Bovine Milk

DOI: 10.3390/ijms231911088

PMID: 36232386
Cancers (2021) IF: 6.639
SIRT3 and Metabolic Reprogramming Mediate the Antiproliferative Effects of Whey in Human Colon Cancer Cells

DOI: 10.3390/cancers13205196

PMID: 34680344
Nutrients (2022) IF: 6.706
Milk Exosomal miR-27b Worsen Endoplasmic Reticulum Stress Mediated Colorectal Cancer Cell Death

DOI: 10.3390/nu14235081
Frontiers in Pharmacology (2022) IF: 5.811
Integrating Gemcitabine-Based Therapy With AdipoRon Enhances Growth Inhibition in Human PDAC Cell Lines.

DOI: 10.3389/fphar.2022.837503
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Phenolic Profiles of Red Wine Relate to Vascular Endothelial Benefits Mediated by SIRT1 and SIRT6

DOI: 10.3390/ijms22115677

PMID: 34073604
Sample: Cell sample
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Colorectal Cancer Apoptosis Induced by Dietary δ-Valerobetaine Involves PINK1/Parkin Dependent-Mitophagy and SIRT3

DOI: 10.3390/ijms22158117

PMID: 34360883
Biomolecules (2020) IF: 4.082
uL3 Mediated Nucleolar Stress Pathway as a New Mechanism of Action of Antiproliferative G-quadruplex TBA Derivatives in Colon Cancer Cells

DOI: 10.3390/biom10040583

PMID: 32290083
Sample: Cell lysate
FEBS LETTERS (2022) IF: 4.124
Diet-derived ergothioneine induces necroptosis in colorectal cancer cells by activating the SIRT3/MLKL pathway

DOI: 10.1002/1873-3468.14310
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
Adipose Tissue and Brain Metabolic Responses to Western Diet—Is There a Similarity between the Two?

DOI: 10.3390/ijms21030786
International Journal of Environmental Research and Public Health (2021) IF: 3.39
Assessment of Unstimulated Whole Salivary Tumor Necrosis Factor Alpha (TNF-α) and Cellular Micronuclei Levels in Snuff (Naswar) Users and Non-Users for Early Diagnosis of Oral Squamous Cell Carcinoma

DOI: 10.3390/ijerph18147230

PMID: 34299679
Sample: saliva
Molecular Medicine Reports (2020) IF: 2.1
Panax notoginseng Saponins protect auditory cells against cisplatin‑induced ototoxicity by inducing the AKT/Nrf2 signaling‑mediated redox pathway

DOI: 10.3892/mmr.2020.11390
Molecular Medicine Reports (2015) IF: 1.554
Effect of rutin on spinal cord injury through inhibition of the expression of MIP-2 and activation of MMP-9, and downregulation of Akt phosphorylation

DOI: 10.3892/mmr.2015.4357

Sample: Cerebrospinal Fluid

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