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F-TESTO(Free Testosterone) ELISA Kit

Name: F-TESTO(Free Testosterone) ELISA Kit
Brand: Elabscience
SKU: E-EL-0165
Price: 495 USD
Availability: InStock

Citations(12)

Cat.No.:E-EL-0165
Reactivity: Universal

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes F-TESTO in samples. No significant cross-reactivity or interference between F-TESTO and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of F-TESTO concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with F-TESTO. During the reaction, F-TESTO in the sample or standard competes with a fixed amount of F-TESTO on the solid phase supporter for sites on the Biotinylated Detection Ab specific to F-TESTO. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of F-TESTO in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level F-TESTO were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level F-TESTO were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	98.04	216.36	884.05	87.82	219.17	800.62
Standard deviation	4.97	10.17	46.59	4.51	10.76	42.43
CV (%)	5.07	4.70	5.27	5.14	4.91	5.30

Recovery

The recovery of F-TESTO spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-105	99
EDTA plasma(n=8)	96-107	103
Cell culture media(n=8)	88-101	94

Linearity

Samples were spiked with high concentrations of F-TESTO and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Epigenetics and Nuclear Signal, Signal transduction, Developmental biology

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021) IF: 6.291
Stereological analysis and transcriptome profiling of testicular injury induced by di-(2-ethylhexyl) phthalate in prepubertal rats

DOI: 10.1016/j.ecoenv.2021.112326

PMID: 34015638
Sample: Serum
SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021) IF: 4.219
Impact of bariatric surgery on androgen profile and ovarian volume in obese polycystic ovary syndrome patients with infertility

DOI: 10.1016/j.sjbs.2021.05.022

PMID: 34466081
Sample: Serum
TOXICOLOGY IN VITRO (2019) IF: 3.067
Competitive endogenous RNA (ceRNA) regulation network of lncRNA-miRNA-mRNA during the process of the nickel-induced steroidogenesis disturbance in rat Leydig cells

DOI: 10.1016/j.tiv.2019.104721

Sample: Cell lysate
REPRODUCTIVE TOXICOLOGY (2015) IF: 3.227
Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes

DOI: 10.1016/j.reprotox.2015.07.079

Sample: Serum
Frontiers in Veterinary Science (2021) IF: 3.412
The Role of Mitochondrial Impairment and Oxidative Stress in the Pathogenesis of Lithium-Induced Reproductive Toxicity in Male Mice

DOI: 10.3389/fvets.2021.603262

PMID: 33842567
Sample: Sperm
BIOLOGICAL TRACE ELEMENT RESEARCH (2017) IF: 3.738
Ameliorative Effect of VE, IGF-I, and hCG on the Fluoride-Induced Testosterone Release Suppression in Mice Leydig Cells

DOI: 10.1007/s12011-017-1023-1

Sample: Cell culture supernatant
Reproductive Biology (2022) IF: 2.376
Oncostatin M and its receptor in women with polycystic ovary syndrome and association with assisted reproductive technology outcomes

DOI: 10.1016/j.repbio.2022.100633

PMID: 35278823
Sample: follicular fluid
Andrology (2017) IF: 2.427
Naringenin attenuates highly active antiretroviral therapy‐induced sperm DNA fragmentations and testicular toxicity in Sprague‐Dawley rats

DOI: 10.1111/andr.12439

Sample: Serum
Andrology (2018) IF: 2.734
Virgin coconut oil extract mitigates testicular‐induced toxicity of alcohol use in antiretroviral therapy

DOI: 10.1111/andr.12490

Sample: Serum
ANDROLOGIA (2017) IF: 1.458
Adjuvant potential of virgin coconut oil extract on antiretroviral therapy‐induced testicular toxicity: An ultrastructural study

DOI: 10.1111/and.12930

Sample: serum
ANDROLOGIA (2018) IF: 1.588
Testicular microanatomical and hormonal alterations following use of antiretroviral therapy in Sprague Dawley rats: Role of Naringenin

DOI: 10.1111/and.13137

PMID: 30168163
Sample: Serum
REPRODUCTION FERTILITY AND DEVELOPMENT (2019) IF: 1.723
Exposure to atrazine during puberty reduces sperm viability, increases weight gain and alters the expression of key metabolic genes in the liver of male mice

DOI: 10.1071/RD18505

PMID: 30636190
Sample: Plasma

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