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Cat.No.:E-EL-0122
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Assay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 1.56-100 pg/mL |
Sensitivity | 0.94 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes fT4 in samples. No significant cross-reactivity or interference between fT4 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of fT4 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level fT4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level fT4 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 5.10 | 11.00 | 44.20 | 5.20 | 11.00 | 45.00 |
Standard deviation | 0.30 | 0.50 | 2.30 | 0.30 | 0.50 | 2.00 |
CV (%) | 5.88 | 4.55 | 5.20 | 5.77 | 4.55 | 4.44 |
The recovery of fT4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 92-106 | 99 |
EDTA plasma(n=8) | 90-101 | 96 |
Cell culture media(n=8) | 96-107 | 102 |
Samples were spiked with high concentrations of fT4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Research Area | Signaling transduction,Developmental Biology |
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
PMID: 34000667 |
Sample: Plasma |
PMID: 34030373 |
Sample: Serum |
PMID: 33304464 |
Sample: serum |
DOI: 10.3390/ijms23158244 |
|
PMID: 35897811 |
Sample: serum |
PMID: 30771657 |
Sample: Plasma |
Sample: plasma |
DOI: 10.1155/2021/2482453 |
|
PMID: 34804362 |
Sample: plasma |
DOI: 10.1530/JME-21-0095 |
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PMID: 34270447 |
Sample: serum |
Sample: Thyroid |
Sample: plasma |
Sample: Plasma |
Sample: Tissue homogenate |
PMID: 36041571 |
Sample: serum |
PMID: 35078449 |
Sample: serum |
Sample: Serum |
Sample: serum |
PMID: 35332411 |
Sample: Serum |
Sample: Serum |
DOI: 10.1111/jfbc.13753 |
|
PMID: 33955004 |
Sample: Serum |
Sample: serum |
Sample: Serum,Tissue homogenate |
Sample: Serum |
Sample: plasma |
Sample: serum |
Sample: Serum |
K***nSubmitted [ Dec 19 2018 ]
Asked: Dear Elabscience, My name is Kevin Chang and I’m a RD who is in charge of reagent design in Skyla corporation. Below is our company website. http://www.skyla.com/index.aspx?lang=US So far, we’re designing our new generation Vet analyzer which will be able to detect free thyroxine (fT4). So, I’m searching for suitable antibody of anti-free thyroxine (fT4) antibody for Canine now. I’m wondering if you have any suitable antibody ? BTW, if there’s any question, please feel free to contact with me. And I’m looking forward to your reply soon.
Reply
Y**iSubmitted [ Dec 19 2018 ]
Answered: Dear Kevin,this is Yumi,glad to get your inquiry.Very sorry for you,this antibody is unavailable in our company,if you have any question later,please feel free to contact me,my email ID is yumi@elabscience.com best regards,