GAP43 Monoclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Mouse
- Reactivity: Human;Mouse;Rat
- Applications: WB;IHC-p
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:Hela,293T,Mouse brain,Rat brain Verified Samples in IHC:Rat brain |
Dilution |
WB 1:1000-2000, IHC 1:50-1:200 Western Blot Operation Guide |
Clonality | Monoclonal |
Immunogen | Recombinant Protein |
Abbre | GAP-43 |
Synonyms | Axonal membrane protein GAP 43;Axonal membrane protein GAP-43;B 50;Calmodulin binding protein P 57;F1;GAP 43;GAP43;Growth Associated Protein 43;Growth-associated protein 43;Nerve Growth Related Peptide;Nerve growth related peptide GAP43;NEUM;Neural phosphoprotein B 50;Neural phosphoprotein B-50;Neuromodulin;Neuron growth associated protein 43;PP46;Protein F1;QtrA-11580;QtrA-13071 |
Swissprot | |
Observed MW |
38,43kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cell membrane. Cell projection>growth cone membrane. Cell junction>synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes. |
Concentration | 1 mg/mL |
Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Purification Method | Protein A purification |
Research Areas | Cancer; Neuroscience |
Clone No. | Clone:6F2 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. |
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Effects of alteplase on neurological deficits and expression of GFAP and GAP-43 in brain tissue of rats with acute cerebral infarction
IF:4.06
Journal:American Journal of Translational Research
PMID:34650733