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GAP43 Monoclonal Antibody

Cat:E-AB-22109 Citations (1)
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Mouse
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p
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Product Details
Verified Samples Verified Samples in WB:Hela,293T,Mouse brain,Rat brain
Verified Samples in IHC:Rat brain
Dilution

WB 1:1000-2000, IHC 1:50-1:200

Western Blot Operation Guide
Clonality Monoclonal
Immunogen Recombinant Protein
Abbre GAP-43
Synonyms Axonal membrane protein GAP 43;Axonal membrane protein GAP-43;B 50;Calmodulin binding protein P 57;F1;GAP 43;GAP43;Growth Associated Protein 43;Growth-associated protein 43;Nerve Growth Related Peptide;Nerve growth related peptide GAP43;NEUM;Neural phosphoprotein B 50;Neural phosphoprotein B-50;Neuromodulin;Neuron growth associated protein 43;PP46;Protein F1;QtrA-11580;QtrA-13071
Swissprot
Observed MW 38,43kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cell membrane. Cell projection>growth cone membrane. Cell junction>synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method Protein A purification
Research Areas Cancer; Neuroscience
Clone No. Clone:6F2
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons.