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Human AD(Adropin) ELISA Kit

Citations(9)Uniprot : Q6UWT2

Cat.No.:E-EL-H5307
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	12.50-800 pg/mL
Sensitivity	7.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human AD in samples. No significant cross-reactivity or interference between Human AD and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human AD concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AD. You can calculate the concentration of Human AD in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human AD were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human AD were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	43.68	95.22	350.77	41.00	89.88	330.27
Standard deviation	2.97	3.93	16.77	2.85	3.79	14.47
CV (%)	6.80	4.13	4.78	6.95	4.22	4.38

Recovery

The recovery of Human AD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-104	97
EDTA plasma (n=8)	89-102	95
Cell culture media (n=8)	89-101	94

Linearity

Samples were spiked with high concentrations of Human AD and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q6UWT2
Synonyms	ENHO, Energy Homeostasis Associated
Research Area	Cardiovascular, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Biomedicines (2023) IF: 4.757
The Impact of SGLT2 Inhibitor Dapagliflozin on Adropin Serum Levels in Men and Women with Type 2 Diabetes Mellitus and Chronic Heart Failure

DOI: 10.3390/biomedicines11020457

Sample: serum
EUROPEAN JOURNAL OF PEDIATRICS (2021) IF: 3.183
Association between adropin and coronary artery lesions in children with Kawasaki disease

DOI: 10.1007/s00431-021-03977-5

PMID: 33712900
Sample: serum
Diagnostics (2022) IF: 3.992
Interplay between Myokine Profile and Glycemic Control in Type 2 Diabetes Mellitus Patients with Heart Failure

DOI: 10.3390/diagnostics12122940

Sample: serum
CLINICAL RHEUMATOLOGY (2018) IF: 2.141
A novel biomarker in patients with knee osteoarthritis: adropin

DOI: 10.1007/s10067-018-4052-z

Sample: Serum
MEDICAL PRINCIPLES AND PRACTICE (2019) IF: 1.103
Serum Adropin Levels Are Reduced in Adult Patients with Nonalcoholic Fatty Liver Disease

DOI: 10.1159/000500106

Sample: serum
Anatolian Journal of Cardiology (2019) IF: 1.112
Association between serum adropin levels and isolated coronary artery ectasia in patients with stable angina pectoris

DOI: 10.14744/AnatolJCardiol.2019.90349

Sample: serum
Canadian Respiratory Journal (2020) IF: 1.639
Alterations in Serum Adropin, Adiponectin, and Proinflammatory Cytokine Levels in OSAS

DOI: 10.1155/2020/2571283

PMID: 32454912
Sample: Serum
CLINICAL AND EXPERIMENTAL HYPERTENSION (2022) IF: 1.749
Serum irisin and adropin levels may be predictors for coronary artery ectasia

DOI: 10.1080/10641963.2021.2018601

PMID: 34994255
Sample: serum
Journal of Cancer Prevention (2018)
Plasma Adropin as a Potential Marker Predicting Obesity and Obesity-associated Cancer in Korean Patients With Type 2 Diabetes Mellitus

DOI: 10.15430/JCP.2018.23.4.191

PMID: 30671402
Sample: Plasma

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