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Human ApoA1(Apolipoprotein A1) ELISA Kit

Citations(6)Uniprot : P02647

Cat.No.:E-EL-H0125
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	3.13-200 ng/mL
Sensitivity	1.88 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human ApoA1 in samples. No significant cross-reactivity or interference between Human ApoA1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human ApoA1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ApoA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ApoA1. You can calculate the concentration of Human ApoA1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ApoA1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ApoA1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	9.30	17.30	76.10	8.90	15.70	83.20
Standard deviation	0.50	1.00	2.70	0.60	0.90	3.80
CV (%)	5.38	5.78	3.55	6.74	5.73	4.57

Recovery

The recovery of Human ApoA1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-105	98
EDTA plasma (n=8)	85-96	91
Cell culture media (n=8)	93-106	98

Linearity

Samples were spiked with high concentrations of Human ApoA1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P02647
Synonyms	APOA1, Apo-A1
Research Area	Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

iScience (2022) IF: 6.107
Selective activation of ABCA1/ApoA1 signalling in the V1 by magnetoelectric stimulation ameliorates depression via regulation of synaptic plasticity

DOI: 10.1016/j.isci.2022.104201

Sample: plasma
Frontiers in Neuroinformatics (2021) IF: 4.081
Bioinformatic Analysis of the Proteome in Exosomes Derived From Plasma: Exosomes Involved in Cholesterol Metabolism Process of Patients With Spinal Cord Injury in the Acute Phase

DOI: 10.3389/fninf.2021.662967

Sample: plasma
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2020) IF: 4.133
Identifying functional non-coding variants in APOA5/A4/C3/A1 gene cluster associated with coronary heart disease

DOI: 10.1016/j.yjmcc.2020.05.003

PMID: 32437778
Sample: Serum
JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 4.791
Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

DOI: 10.1016/j.jpsychires.2022.04.002

PMID: 35429740
Sample: blood
LIFE SCIENCES (2020) IF: 3.647
Evaluation of circulating miR-122, miR-30c and miR-33a levels and their association with lipids, lipoproteins in postprandial lipemia

DOI: 10.1016/j.lfs.2020.118585

PMID: 33058914
Sample: Serum
Clinical Diabetology (2022)
Assessment of Hepatobiliary Status and Cardiometabolic Risk Markers among Type 2 Diabetes Mellitus Patients. A Cross-Sectional Study

DOI: 10.5603/DK.a2022.0058

Sample: serum

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