Current Position:Home>>Kits>>ELISA Kits>> Human CGRP1(Calcitonin Gene Related Peptide 1) ELISA Kit
Cat.No.:E-EL-H0619
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human CGRP1 in samples. No significant cross-reactivity or interference between Human CGRP1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human CGRP1 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CGRP1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CGRP1 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 48.15 | 145.75 | 349.43 | 51.54 | 133.87 | 361.48 |
Standard deviation | 3.10 | 8.31 | 13.66 | 3.40 | 6.84 | 13.59 |
CV (%) | 6.44 | 5.70 | 3.91 | 6.60 | 5.11 | 3.76 |
The recovery of Human CGRP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 89-103 | 96 |
EDTA plasma (n=8) | 87-98 | 92 |
Cell culture media (n=8) | 91-105 | 96 |
Samples were spiked with high concentrations of Human CGRP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P06881
|
Synonyms | CGRP1 |
Research Area | Metabolism, Signal transduction, Cancer |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
PMID: 36395769 |
Sample: plasma |
DOI: 10.1039/D1FO03896F |
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Sample: serum |
DOI: 10.1155/2019/2363420 |
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Sample: serum |
J**********dSubmitted [ Oct 13 2023 ]
Asked: Is extraction required for the samples being used with this kit or just dilution?
Reply
adminSubmitted [ Oct 13 2023 ]
Answered: Hi Jess, for secretory samples, such as serum/plasma, it is generally recommended not to dilute the test and there are no extraction steps. Serum/plasma samples should be prepared as follows: Serum: Allow samples to clot for 1 hour at room temperature or overnight at 2-8℃beforecentrifugation for 20 min at 1000×g at 2-8℃. Collect the supernatant to carry out the assay. Plasma: Collect plasma using EDTA-Na2 as an anticoagulant. Centrifuge samples for 15min at 1000×g at 2-8℃ within 30 min of collection. Collect the supernatant to carry outthe assay. If you need any other assistance, please let us know. Have a nice day!