Current Position:Home>>Kits>>ELISA Kits>> Human FGF23(Fibroblast Growth Factor 23) ELISA Kit

Human FGF23(Fibroblast Growth Factor 23) ELISA Kit

Citations(16)Uniprot : Q9GZV9

Cat.No.:E-EL-H1116
Reactivity: Human

Filter:

ELISA

To Purchase E-EL-H1116

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human FGF23 in samples. No significant cross-reactivity or interference between Human FGF23 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human FGF23 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGF23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGF23. You can calculate the concentration of Human FGF23 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human FGF23 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human FGF23 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	50.86	87.87	437.81	48.92	79.96	443.72
Standard deviation	3.31	4.66	19.96	2.75	3.49	20.85
CV (%)	6.51	5.30	4.56	5.62	4.36	4.70

Recovery

The recovery of Human FGF23 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-110	101
EDTA plasma (n=8)	96-107	102
Cell culture media (n=8)	89-104	95

Linearity

Samples were spiked with high concentrations of Human FGF23 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q9GZV9
Synonyms	FGF-23, ADHR, FGFN, HYPF, HPDR2, PHPTC
Research Area	Cancer, Developmental biology, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

eLife (2020) IF: 7.08
High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

DOI: 10.7554/eLife.52709

PMID: 32271147
Sample: Serum
Frontiers in Neuroscience (2021) IF: 4.677
Kernelized k-Local Hyperplane Distance Nearest-Neighbor Model for Predicting Cerebrovascular Disease in Patients With End-Stage Renal Disease

DOI: 10.3389/fnins.2021.773208

PMID: 34759797
Sample: serum
KIDNEY & BLOOD PRESSURE RESEARCH (2018) IF: 3
The Association of Fibroblast Growth Factor 23 with Arterial Stiffness and Atherosclerosis in Patients with Autosomal Dominant Polycystic Kidney Disease

DOI: 10.1159/000492244

Sample: serum
PLoS One (2023) IF: 3.752
Correlation study of FGF23/D-serine in maintenance hemodialysis patients with combined hearing impairment

DOI: 10.1371/journal.pone.0280378

Sample: serum
JOURNAL OF NEPHROLOGY (2021) IF: 3.902
Body composition, adipokines, FGF23-Klotho and bone in kidney transplantation: Is there a link?

DOI: 10.1007/s40620-021-00972-9

PMID: 33560479
Sample: Serum
BLOOD PURIFICATION (2020) IF: 2.116
Comparison of Circulating Levels of Uremic Toxins in Hemodialysis Patients Treated with Medium Cut-Off Membranes and High-Flux Membranes: Theranova in Sisli Hamidiye Etfal (THE SHE) Randomized Control Study

DOI: 10.1159/000508061

PMID: 32634815
Sample: Serum
RHEUMATOLOGY INTERNATIONAL (2019) IF: 2.2
Evaluation of serum fibroblast growth factor-23 in patients with axial spondyloarthritis and its association with sclerostin, inflammation, and spinal damage

DOI: 10.1007/s00296-019-04298-5

PMID: 30968207
Sample: Serum
Computational and Mathematical Methods in Medicine (2021) IF: 2.238
A Self-Representation-Based Fuzzy SVM Model for Predicting Vascular Calcification of Hemodialysis Patients

DOI: 10.1155/2021/2464821

PMID: 34367315
Sample: serum
JOURNAL OF CLINICAL DENSITOMETRY (2020) IF: 2.31
Association Between FGF-23 Levels and Risk of Fracture in Women With Systemic Sclerosis

DOI: 10.1016/j.jocd.2020.05.010

PMID: 32600830
Sample: Serum
JOURNAL OF BONE AND MINERAL METABOLISM (2021) IF: 2.626
Association of plasma neutrophil gelatinase-associated lipocalin with parameters of CKD–MBD in maintenance hemodialysis patients

DOI: 10.1007/s00774-021-01248-9

Sample: plasma
BMJ Paediatrics Open (2023) IF: 2.926
Fibroblast growth factor 23 in children with or without heart failure: a prospective study

DOI: 10.1136/bmjpo-2022-001753

Sample: serum
PeerJ (2021) IF: 2.984
The correlation between fibroblast growth factor-23 and ESRD patients with hearing impairment

DOI: 10.7717/peerj.12295

PMID: 34721981
Sample: serum
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION (2020) IF: 1.475
Association of serum FGF-23, klotho, fetuin-A, osteopontin, osteoprotegerin and hs-CRP levels with coronary artery disease

DOI: 10.1080/00365513.2020.1728786

Sample: serum
THERAPEUTIC APHERESIS AND DIALYSIS (2017) IF: 1.529
FGF23 Level and Intima‐Media Thickness Are Elevated From Early Stages of Chronic Kidney Disease

DOI: 10.1111/1744-9987.12592

Sample: Serum
Endokrynologia Polska (2021) IF: 1.582
New insights into the metabolic-bone crosstalk in active acromegaly

DOI: 10.5603/EP.a2021.0028

Sample: serum
INDIAN JOURNAL OF PEDIATRICS (2021) IF: 1.967
Fibroblast Growth Factor 23 Level and Cardiovascular Parameters in Children with Chronic Kidney Disease

DOI: 10.1007/s12098-021-03927-x

PMID: 34767187
Sample: Serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha