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Human GAL3(Galectin 3) ELISA Kit

Citations(16)Uniprot : P17931

Cat.No.:E-EL-H1470
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human GAL3 in samples. No significant cross-reactivity or interference between Human GAL3 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human GAL3 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GAL3. You can calculate the concentration of Human GAL3 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human GAL3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human GAL3 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.55	1.03	3.99	0.54	0.96	3.64
Standard deviation	0.04	0.05	0.18	0.03	0.05	0.20
CV (%)	7.27	4.85	4.51	5.56	5.21	5.49

Recovery

The recovery of Human GAL3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-104	99
EDTA plasma (n=8)	89-104	96
Cell culture media (n=8)	94-107	100

Linearity

Samples were spiked with high concentrations of Human GAL3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P17931
Synonyms	LGALS3, CBP35, GALBP, GALIG, L31, LGALS2, MAC2, Lectin,Galactoside-Binding Soluble 3
Research Area	Cancer, Cell Biology, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Biomolecules (2023) IF: 6.064
FK506-Binding Protein like (FKBPL) Has an Important Role in Heart Failure with Preserved Ejection Fraction Pathogenesis with Potential Diagnostic Utility

DOI: 10.3390/biom13020395

Sample: serum
JOURNAL OF PERIODONTOLOGY (2021) IF: 6.993
Evaluation of galectin-3, peptidylarginine deiminase-4 and tumor necrosis factor-α levels in gingival crevicular fluid for periodontal health, gingivitis and stage III grade C periodontitis: A pilot study

DOI: 10.1002/JPER.21-0137

PMID: 33913157
Sample: Gingival Crevicular Fluid
JOURNAL OF CARDIOVASCULAR MAGNETIC RESONANCE (2020) IF: 5.361
Imaging, biomarker and invasive assessment of diffuse left ventricular myocardial fibrosis in atrial fibrillation

DOI: 10.1186/s12968-020-0603-y

PMID: 32036784
Sample: Whole Blood
Genes (2022) IF: 4.141
Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event

DOI: 10.3390/genes14010109
Biomolecules (2019) IF: 4.694
Clinical Value of Circulating Microribonucleic Acids miR-1 and miR-21 in Evaluating the Diagnosis of Acute Heart Failure in Asymptomatic Type 2 Diabetic Patients.

DOI: 10.3390/biom9050193

Sample: serum
Frontiers in Physiology (2020) IF: 3.367
Elevated β1-Adrenergic Receptor Autoantibody Levels Increase Atrial Fibrillation Susceptibility by Promoting Atrial Fibrosis

DOI: 10.3389/fphys.2020.00076

Sample: Serum
International Journal of Environmental Research and Public Health (2022) IF: 3.39
How Diabetes and Other Comorbidities of Elderly Patients and Their Treatment Influence Levels of Glycation Products

DOI: 10.3390/ijerph19127524

PMID: 35742776
Sample: serum
Diagnostics (2021) IF: 3.706
Diagnostic Performance of Serum Biomarkers Fibroblast Growth Factor 21, Galectin-3 and Copeptin for Heart Failure with Preserved Ejection Fraction in a Sample of Patients with Type 2 Diabetes Mellitus

DOI: 10.3390/diagnostics11091577

PMID: 34573919
Sample: serum
CLINICAL BIOCHEMISTRY (2016) IF: 2.382
Circulating biomarkers of fibrosis and cardioversion of atrial fibrillation: A prospective, controlled cohort study

DOI: 10.1016/j.clinbiochem.2016.09.008

Sample: Serum
RHEUMATOLOGY INTERNATIONAL (2021) IF: 2.631
Galectin-3: a new biomarker for differentiating periodic fever, adenitis, pharyngitis, aphthous stomatitis (PFAPA) syndrome from familial Mediterranean fever?

DOI: 10.1007/s00296-021-04827-1

PMID: 33709179
Sample: Serum
PLoS One (2018) IF: 2.766
Left atrial voltage, circulating biomarkers of fibrosis, and atrial fibrillation ablation. A prospective cohort study

DOI: 10.1371/journal.pone.0189936

Sample: serum
Medicina-Lithuania (2022) IF: 2.948
Changes in Left Heart Geometry, Function, and Blood Serum Biomarkers in Patients with Obstructive Sleep Apnea after Treatment with Continuous Positive Airway Pressure

DOI: 10.3390/medicina58111511

PMID: 36363468
Sample: serum
Molecular Medicine Reports (2021) IF: 2.952
Galectin‑3 facilitates the proliferation and migration of nasopharyngeal carcinoma cells via activation of the ERK1/2 and Akt signaling pathways, and is positively correlated with the inflammatory state of nasopharyngeal carcinoma

DOI: 10.3892/mmr.2021.12009

PMID: 33760180
Sample: serum
Psychiatry Investigation (2020) IF: 1.688
Serum Galectin-3 Levels in Children with Attention-Deficit/Hyperactivity Disorder

DOI: 10.30773/pi.2019.0247

PMID: 32151128
Sample: Serum
Brazilian Journal of Psychiatry (2020) IF: 1.904
Serum galectin-3 levels are decreased in schizophrenia

DOI: 10.1590/1516-4446-2019-0699

PMID: 32159713
Sample: Serum
Journal of Laboratory Physicians (2020)
Evaluation of Galectin-3 as a Novel Diagnostic Biomarker in Patients with Heart Failure with Preserved Ejection Fraction

DOI: 10.1055/s-0040-1716608

Sample: serum

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