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Human GH(Growth Hormone) ELISA Kit

Citations(3)Uniprot : P01241

Cat.No.:E-EL-H0177
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	78.13-5000 pg/mL
Sensitivity	46.88 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human GH in samples. No significant cross-reactivity or interference between Human GH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human GH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GH. You can calculate the concentration of Human GH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human GH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human GH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	260.50	733.10	2028.80	238.50	668.30	2208.90
Standard deviation	14.30	40.30	87.20	13.60	35.40	114.90
CV (%)	5.49	5.50	4.30	5.70	5.30	5.20

Recovery

The recovery of Human GH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-105	99
EDTA plasma (n=8)	94-106	100
Cell culture media (n=8)	88-99	94

Linearity

Samples were spiked with high concentrations of Human GH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P01241
Synonyms	GH1, GH-N, GHN, hGH-N, Somatotropin
Research Area	Cancer, Developmental biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Frontiers in Nutrition (2022) IF: 6.59
Sleep duration of lactating mothers and its relationship with feeding pattern, milk macronutrients and related serum factors: A combined longitudinal cohort and cross-sectional study

DOI: 10.3389/fnut.2022.973291

PMID: 36110402
Sample: plasma
SCIENCE OF THE TOTAL ENVIRONMENT (2019) IF: 5.589
Distribution, metabolism and hepatotoxicity of neonicotinoids in small farmland lizard and their effects on GH/IGF axis

DOI: 10.1016/j.scitotenv.2019.01.277

PMID: 30795479
Sample: Plasma
Endokrynologia Polska (2021) IF: 1.582
New insights into the metabolic-bone crosstalk in active acromegaly

DOI: 10.5603/EP.a2021.0028

Sample: serum

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Reviews/Q&A

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Q&A

M************mSubmitted [ Aug 05 2022 ]

Asked: With respect to the above question, the analyte of interest will be specific to Human. Human growth hormone will be administered to a rodent or non rodent animal and plasma from the respective species will be used as the blood from the animals will have the human growth hormone. The only difference is the matrix of the species. Will the matrix of other species affect the assay ?

adminSubmitted [ Aug 05 2022 ]

Answered: We have not done experiments to verify this, but since different species may have small differences in the internal environment. We speculate that substrate interference is very likely.

M************mSubmitted [ Aug 04 2022 ]

Asked: Can this Kit be used for biological fluids from other than Human? Like, determination of Human growth hormone in Rat/ rabbit or swine plasma ?

adminSubmitted [ Aug 04 2022 ]

Answered:

adminSubmitted [ Aug 04 2022 ]

Answered: For this kit, we designed it specifically for human, and it is not recommended to be used for testing samples of other species. If the you want to test the rat sample, we have E-EL-R3003.

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