Current Position:Home>>Kits>>ELISA Kits>> Human GROβ/CXCL2(Growth Regulated Oncogene Beta) ELISA Kit

Human GROβ/CXCL2(Growth Regulated Oncogene Beta) ELISA Kit

Citations(7)Uniprot : P19875

Cat.No.:E-EL-H1904
Reactivity: Human

Filter:

ELISA

To Purchase E-EL-H1904

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human GROβ/CXCL2 in samples. No significant cross-reactivity or interference between Human GROβ/CXCL2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human GROβ/CXCL2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GROβ/CXCL2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GROβ/CXCL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GROβ/CXCL2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GROβ/CXCL2. You can calculate the concentration of Human GROβ/CXCL2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human GROβ/CXCL2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human GROβ/CXCL2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	54.70	135.50	382.40	59.70	146.50	360.80
Standard deviation	3.70	7.60	15.30	3.00	6.60	17.70
CV (%)	6.76	5.61	4.00	5.03	4.51	4.91

Recovery

The recovery of Human GROβ/CXCL2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-106	98
EDTA plasma (n=8)	91-104	97
Cell culture media (n=8)	86-97	91

Linearity

Samples were spiked with high concentrations of Human GROβ/CXCL2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P19875
Synonyms	CXCL2, MIP-2a, GRO2, SCYB2, GROb, MGSA-B, CINC2a
Research Area	Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Nature Communications (2022) IF: 17.694
ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression

DOI: 10.1038/s41467-022-34871-9

PMID: 36435834
Sample: serum,prostate tissue
ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119
LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

DOI: 10.1002/tox.23464

PMID: 35006638
Sample: A549 cell
Journal of the International Society of Sports Nutrition (2019) IF: 3.841
Effects of a polysaccharide-based multi-ingredient supplement on salivary immunity in non-elite marathon runners

DOI: 10.1186/s12970-019-0281-z

Sample: saliva
EUROPEAN JOURNAL OF MEDICAL RESEARCH (2022) IF: 2.175
Changes in the BTK/NF-κB signaling pathway and related cytokines in different stages of neuromyelitis optica spectrum disorders

DOI: 10.1186/s40001-022-00723-x

PMID: 35729649
Sample: blood
PLoS One (2018) IF: 2.766
Salivary immunity and lower respiratory tract infections in non-elite marathon runners

DOI: 10.1371/journal.pone.0206059

PMID: 30462646
Sample: saliva
EUROPEAN JOURNAL OF DERMATOLOGY (2022) IF: 2.805
Role of the long non-coding RNA, SPRR2C, based on an in vitro psoriatic keratinocyte cell model

DOI: 10.1684/ejd.2022.4247

PMID: 35866915
Sample: Ker-CT cell,HaCaT cell
JOURNAL OF SPINAL CORD MEDICINE (2019) IF: 1.711
Cytokine–chemokine and cognitive profile of multiple sclerosis patients with predominant optic nerve and spinal cord involvement

DOI: 10.1080/10790268.2019.1666238

Sample: serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha