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Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit

Citations(18)Uniprot : P05019

Cat.No.:E-EL-H0086
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human IGF-1 in samples. No significant cross-reactivity or interference between Human IGF-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human IGF-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IGF-1. You can calculate the concentration of Human IGF-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IGF-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IGF-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	5.30	11.80	48.60	5.60	10.90	47.70
Standard deviation	0.30	0.50	1.90	0.30	0.50	1.80
CV (%)	5.66	4.24	3.91	5.36	4.59	3.77

Recovery

The recovery of Human IGF-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-106	100
EDTA plasma (n=8)	92-108	99
Cell culture media (n=8)	86-99	92

Linearity

Samples were spiked with high concentrations of Human IGF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05019
Synonyms	IGF1, IGFI, IGF-I, IGF1A, IGF-IA, IGF-IB, MGF, Somatomedin C
Research Area	Cancer, Developmental biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

CANCER LETTERS (2021) IF: 8.679
LncCCLM inhibits lymphatic metastasis of cervical cancer by promoting STAU1-mediated IGF-1 mRNA degradation

DOI: 10.1016/j.canlet.2021.07.005

PMID: 34273467
Sample: CC cells
Stem Cells International (2021) IF: 5.443
Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway

DOI: 10.1155/2021/5553676

PMID: 34912459
Sample: LFC
Cell Death & Disease (2019) IF: 5.959
Insulin-like growth factor-1 attenuates oxidative stress-induced hepatocyte premature senescence in liver fibrogenesis via regulating nuclear p53–progerin interaction

DOI: 10.1038/s41419-019-1670-6

Sample: plasm
JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS (2021) IF: 4.291
Comparison of Serum VEGF, IGF-1, and HIF-1α Levels in Children with Autism Spectrum Disorder and Healthy Controls

DOI: 10.1007/s10803-020-04820-w

PMID: 33389301
Sample: Serum
Scientific Reports (2021) IF: 4.38
Obesity-related proteins score as a potential marker of breast cancer risk

DOI: 10.1038/s41598-021-87583-3

PMID: 33859244
Sample: Plasma
Journal of Hepatocellular Carcinoma (2020) IF: 4.655
Prognostic Significance of Serum Insulin-Like Growth Factor-1 in Hepatocellular Cancer Patients: A Validation Study

DOI: 10.2147/JHC.S258930

Sample: serum
Aging-US (2020) IF: 4.831
MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition

DOI: 10.18632/aging.103818

PMID: 32805717
Sample: Cell sample
JOURNAL OF MOLECULAR ENDOCRINOLOGY (2023) IF: 4.869
CTCF variant begets to short stature by down-regulation of IGF-1

DOI: 10.1530/JME-22-0193

Sample: HEK-293T cell,LO2 cell,immortalized lymphocyte cel
NEUROSCIENCE LETTERS (2022) IF: 3.046
Role of IGF-1 in neuroinflammation and cognition deficits induced by sleep deprivation

DOI: 10.1016/j.neulet.2022.136575

PMID: 35276231
Sample: Plasma
DIABETES RESEARCH AND CLINICAL PRACTICE (2019) IF: 3.239
Ramadan diurnal intermittent fasting modulates SOD2, TFAM, Nrf2, and sirtuins (SIRT1, SIRT3) gene expressions in subjects with overweight and obesity

DOI: 10.1016/j.diabres.2019.107801

Sample: serum
DIABETES RESEARCH AND CLINICAL PRACTICE (2019) IF: 3.239
Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals

DOI: 10.1016/j.diabres.2019.05.023

PMID: 31150725
Sample: Serum
Frontiers in Nutrition (2020) IF: 3.365
A Novel 7-Days Prolonged Dietary Deprivation Regimen Improves ALT and UA After 3–6 Months Refeeding, Indicating Therapeutic Potential

DOI: 10.3389/fnut.2020.00050

Sample: serum
GROWTH HORMONE & IGF RESEARCH (2022) IF: 2.372
In missed abortion the decrease of IGF-1 down-regulates PI3K/AKT signaling pathway reducing the secretion of progesterone and β-hCG

DOI: 10.1016/j.ghir.2022.101479

PMID: 35752133
Sample: serum
INVESTIGATIONAL NEW DRUGS (2019) IF: 2.663
The rhenium(I)-diselenoether anticancer drug targets ROS, TGF-β1, VEGF-A, and IGF-1 in an in vitro experimental model of triple-negative breast cancers

DOI: 10.1007/s10637-019-00727-1

PMID: 30632005
Sample: Cell culture medium
ACTA DIABETOLOGICA (2019) IF: 2.996
polymorphisms with diabetic retinopathy in Pakistani population

DOI: 10.1007/s00592-019-01407-5

Sample: Serum
AURIS NASUS LARYNX (2020) IF: 1.436
Association between adenotonsillar hypertrophy and leptin, ghrelin and IGF-1 levels in children

DOI: 10.1016/j.anl.2020.08.002

Sample: serum
Endokrynologia Polska (2021) IF: 1.582
New insights into the metabolic-bone crosstalk in active acromegaly

DOI: 10.5603/EP.a2021.0028

Sample: serum
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES (2016) IF: 0.756
Effect of KiFAY on Performance, Insulin-like Growth Factor-1, and Thyroid Hormones in Broilers

DOI: 10.5713/ajas.16.0188

Sample: Serum

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