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Human IL-17A(Interleukin 17A) ELISA Kit

Citations(31)Uniprot : Q16552

  • Cat.No.:E-EL-H0105

  • Reactivity: Human
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-17A in samples. No significant cross-reactivity or interference between Human IL-17A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-17A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-17A. You can calculate the concentration of Human IL-17A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-17A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-17A were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 95.20 215.10 996.10 94.90 200.40 990.40
Standard deviation 6.30 10.10 37.90 6.00 9.20 50.50
CV (%) 6.62 4.70 3.80 6.32 4.59 5.10

Recovery

The recovery of Human IL-17A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-99 93
EDTA plasma (n=8) 85-97 90
Cell culture media (n=8) 86-101 93

Linearity

Samples were spiked with high concentrations of Human IL-17A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   Q16552
SynonymsCTLA8, CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17, IL17A, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Theranostics (2021) IF: 11.556
    A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

    DOI: 10.7150/thno.58321

    PMID: 33995679

    		 Sample: Tissue homogenate
  2. ACTA PHARMACOLOGICA SINICA (2021) IF: 6.15
    Interleukin-17 upregulation participates in the pathogenesis of heart failure in mice via NF-κB-dependent suppression of SERCA2a and Cav1.2 expression

    DOI: 10.1038/s41401-020-00580-6

    PMID: 33589793

    		 Sample: Plasma
  3. Food & Function (2022) IF: 6.317
    Whole grain germinated brown rice regulates intestinal immune homeostasis and gastrointestinal hormones in type 2 diabetic patients—a randomized control trial

    DOI: 10.1039/D2FO00477A
    Sample: serum
  4. Frontiers in Cell and Developmental Biology (2021) IF: 6.684
    Fexofenadine Protects Against Intervertebral Disc Degeneration Through TNF Signaling

    DOI: 10.3389/fcell.2021.687024
    Sample: NP cell
  5. OSTEOARTHRITIS AND CARTILAGE (2018) IF: 5.454
    Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis

    DOI: 10.1016/j.joca.2018.05.003
    Sample: Serum
  6. Nutrients (2021) IF: 5.719
    Rapid and Effective Vitamin D Supplementation May Present Better Clinical Outcomes in COVID-19 (SARS-CoV-2) Patients by Altering Serum INOS1, IL1B, IFNg, Cathelicidin-LL37, and ICAM1

    DOI: 10.3390/nu13114047

    PMID: 34836309

    		 Sample: serum
  7. CLINICAL ORAL IMPLANTS RESEARCH (2021) IF: 5.977
    Notch down-regulation and inflammatory cytokines and RANKL overexpression involvement in peri-implant mucositis and peri-implantitis: A cross-sectional study

    DOI: 10.1111/clr.13850

    PMID: 34546593

    		 Sample: peri-implant crevicular fluid
  8. MEDIATORS OF INFLAMMATION (2021) IF: 4.711
    The lncRNA H19/miR-766-3p/S1PR3 Axis Contributes to the Hyperproliferation of Keratinocytes and Skin Inflammation in Psoriasis via the AKT/mTOR Pathway

    DOI: 10.1155/2021/9991175

    PMID: 34992498

    		 Sample: HaCaT cell
  9. Pharmacological Reports (2021) IF: 3.027
    The association of the quality of sleep with proinflammatory cytokine profile in inflammatory bowel disease patients

    DOI: 10.1007/s43440-021-00333-0

    PMID: 34694623

    		 Sample: serum
  10. Oncology Letters (2022) IF: 3.111
    A role for serum cytokines and cell adhesion molecules in the non‑invasive diagnosis of colorectal cancer

    DOI: 10.3892/ol.2022.13443
    Sample: serum
  11. INTERNATIONAL IMMUNOPHARMACOLOGY (2018) IF: 3.118
    Novel anti-inflammatory target of geniposide: Inhibiting Itgβ1/Ras-Erk1/2 signal pathway via the miRNA-124a in rheumatoid arthritis synovial fibroblasts

    DOI: 10.1016/j.intimp.2018.09.049
    Sample: MH7A cells
  12. BMC Pulmonary Medicine (2021) IF: 3.317
    Systemic and local cytokine profile and risk factors for persistent allergic airway inflammation in patients sensitised to house dust mite allergens

    DOI: 10.1186/s12890-021-01798-8

    PMID: 34930201

    		 Sample: serum,nasal lavage
  13. CLINICAL BIOCHEMISTRY (2022) IF: 3.625
    Expression of Nrf2 protein in serum of patients with rheumatoid arthritis: A novel indicator for disease activity and disease prognosis

    DOI: 10.1016/j.clinbiochem.2022.12.011
    Sample: serum
  14. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2019) IF: 2.086
    In vitro impact of bisphenol A on maturation and function of monocyte-derived dendritic cells in patients with primary Sjögren’s syndrome

    DOI: 10.1080/08923973.2019.1706554
    Sample: PBMCs
  15. Sleep and Breathing (2020) IF: 2.162
    Intermittent hypoxia induces tumor immune escape in murine S180 solid tumors via the upregulation of TGF-β 1 in mice

    DOI: 10.1007/s11325-020-02166-2

    PMID: 32840731

    		 Sample: Tissue homogenate
  16. LUPUS (2020) IF: 2.251
    Cytokines, 25-OH vit D and disease activity in patients with juvenile-onset systemic lupus erythematosus:

    DOI: 10.1177/0961203320973068
    Sample: Plasma
  17. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2020) IF: 2.352
    A case of asthma with Behcet’s disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions

    DOI: 10.1080/08923973.2020.1789656
    Sample: Serum
  18. CLINICAL RHEUMATOLOGY (2020) IF: 2.394
    The clinical value of interleukins-8, -10, and -17 in idiopathic granulomatous mastitis

    DOI: 10.1007/s10067-020-04925-8
    Sample: blood
  19. American Journal of Rhinology & Allergy (2021) IF: 2.467
    Immunosuppressive Regulation of Dendritic Cells and T Cells in Allergic Rhinitis by Semaphorin 3A:

    DOI: 10.1177/19458924211005592
    Sample: Cell
  20. Translational Pediatrics (2021) IF: 2.488
    Comparison of safety, effectiveness and serum inflammatory factor indexes of Saccharomyces boulardii versus Bifidobacterium triple viable in treating children with chronic diarrhea: a randomized trial

    DOI: 10.21037/tp-21-195
    Sample: serum
  21. BIOSCIENCE REPORTS (2019) IF: 2.535
    Correlations of IL-17 and NF-κB gene polymorphisms with susceptibility and prognosis in acute respiratory distress syndrome in a chinese population

    DOI: 10.1042/BSR20181987

    PMID: 30655311

    		 Sample: Serum
  22. SCANDINAVIAN JOURNAL OF IMMUNOLOGY (2020) IF: 2.717
    Early HIV infection is associated with reduced proportions of gamma delta T subsets as well as high creatinine and urea levels

    DOI: 10.1111/sji.12868

    PMID: 32052490

    		 Sample: Serum
  23. LUPUS (2021) IF: 2.911
    Association of microRNA-125a with the clinical features, disease activity and inflammatory cytokines of juvenile-onset lupus patients:

    DOI: 10.1177/09612033211010328
    Sample: Plasma
  24. CYTOKINE (2020) IF: 2.952
    Involvement of the cytokine TWEAK in the pathogenesis of psoriasis vulgaris, pustular psoriasis, and erythrodermic psoriasis

    DOI: 10.1016/j.cyto.2020.155391

    PMID: 33302223

    		 Sample: serum
  25. Journal of Cosmetic Dermatology (2019) IF: 1.311
    Serum interleukin‐17 as a novel biomarker in patients with acne vulgaris

    DOI: 10.1111/jocd.12934
    Sample: Serum
  26. CLINICAL AND EXPERIMENTAL DERMATOLOGY (2017) IF: 1.589
    Serum IL‐17 in patients with erythema multiforme or Stevens–Johnson syndrome/toxic epidermal necrolysis drug reaction, and correlation with disease severity

    DOI: 10.1111/ced.13213
    Sample: Serum
  27. Journal of Obsessive-Compulsive and Related Disorders (2021) IF: 1.677
    TGF-β1, neopterin, tetrahydrobiopterin, and nitric oxide levels in pediatric obsessive–compulsive disorder

    DOI: 10.1016/j.jocrd.2021.100649
    Sample: Serum
  28. JOURNAL OF INVESTIGATIVE SURGERY (2020) IF: 1.685
    New Findings on Autoimmune Etiology of Idiopathic Granulomatous Mastitis: Serum IL-17, IL-22 and IL-23 Levels of Patients

    DOI: 10.1080/08941939.2020.1725190
    Sample: serum
  29. EUROPEAN CYTOKINE NETWORK (2019) IF: 1.875
    Elevated cytokine levels associated with acute kidney injury due to wasp sting

    DOI: 10.1684/ecn.2019.0425
    Sample: serum
  30. Annals of Medicine and Surgery (2022)
    The roles of Single Nucleotide Polymorphism (SNP) Endoplasmic Reticulum Aminopeptidase 1 (ERAP 1) gene in axial spondyloarthritis Indonesian adults

    DOI: 10.1016/j.amsu.2022.103675

    PMID: 35638067

    		 Sample: peripheral blood mononuclear cell (PBMC)
  31. Revista da Associacao Medica Brasileira (2020) IF: 0.915
    A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic inflammatory demyelinating polyneuropathy

    DOI: 10.1590/1806-9282.66.11.1583
    Sample: cerebrospinal fluid (CSF)
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

a**nSubmitted [ Aug 30 2022 ]

Asked: What is the optimum dilution fold for the serum sample?

Reply

A

adminSubmitted [ Aug 30 2022 ]

Answered: For E-EL-H0105, the optimum dilution fold for the serum sample is 1:1.

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