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Human IL-2(Interleukin 2) ELISA Kit

Citations(22)Uniprot : P60568

  • Cat.No.:E-EL-H0099

  • Reactivity: Human
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human IL-2 in samples. No significant cross-reactivity or interference between Human IL-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human IL-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-2. You can calculate the concentration of Human IL-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 23.64 58.38 217.66 23.12 56.78 226.32
Standard deviation 1.29 2.76 11.30 1.26 2.61 9.30
CV (%) 5.46 4.73 5.19 5.45 4.60 4.11

Recovery

The recovery of Human IL-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 93
EDTA plasma (n=8) 93-104 100
Cell culture media (n=8) 93-102 99

Linearity

Samples were spiked with high concentrations of Human IL-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P60568
SynonymsIL2, TCGF, Lymphokine
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2020) IF: 11.161
    MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8 + T lymphocytes in human diffuse large B cell lymphoma

    DOI: 10.1186/s13046-020-01752-2

    PMID: 33168024

    		 Sample: Cell culture supernatant
  2. Acta Pharmaceutica Sinica B (2021) IF: 11.614
    Abrogation of HnRNP L enhances anti-PD-1 therapy efficacy via diminishing PD-L1 and promoting CD8+ T cell-mediated ferroptosis in castration-resistant prostate cancer

    DOI: 10.1016/j.apsb.2021.07.016
    Sample: PC3 cells,DU145 cells
  3. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021) IF: 6.291
    miR-122–5p regulates hepatocytes damage caused by BaP and DBP co-exposure through SOCS1/STAT3 signaling in vitro

    DOI: 10.1016/j.ecoenv.2021.112570
    Sample: L02 cell
  4. JOURNAL OF BIOLOGICAL CHEMISTRY (2017) IF: 5.157
    Down-regulation of microRNA-451a facilitates the activation and proliferation of CD4+ T cells by targeting Myc in patients with dilated cardiomyopathy

    DOI: 10.1074/jbc.M116.765107
    Sample: Cell culture supernatant
  5. JOURNAL OF CELLULAR PHYSIOLOGY (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    		 Sample: Cell culture supernatant
  6. WORLD JOURNAL OF GASTROENTEROLOGY (2021) IF: 5.742
    Serum soluble suppression of tumorigenicity 2 as a novel inflammatory marker predicts the severity of acute pancreatitis

    DOI: 10.3748/wjg.v27.i38.6489

    PMID: 34720537

    		 Sample: serum
  7. Frontiers in Pharmacology (2022) IF: 5.811
    Berberine Inhibits the Adhesion of Candida albicans to Vaginal Epithelial Cells

    DOI: 10.3389/fphar.2022.814883

    PMID: 35295335

    		 Sample: vaginal epithelial cell
  8. JOURNAL OF CLINICAL PERIODONTOLOGY (2019) IF: 4.164
    Effects of non‐surgical periodontal therapy on inflammatory markers of psoriasis: A randomized controlled trial

    DOI: 10.1111/jcpe.13205
    Sample: saliva
  9. OncoTargets and Therapy (2020) IF: 3.337
    MiRNA505/NET1 Axis Acts as a CD8+ T-TIL Regulator in Non-Small Cell Lung Cancer

    DOI: 10.2147/OTT.S265859
    Sample: Cell culture supernatant
  10. CELL BIOLOGY INTERNATIONAL (2022) IF: 3.612
    The positive feedback loop of furin and TGFβ1 enhances the immune responses of Tregs to hepatocellular carcinoma cells and hepatitis B virus in vitro

    DOI: 10.1002/cbin.11806

    PMID: 35349767

    		 Sample: HepG2 cell
  11. JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022) IF: 3.657
    Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?

    DOI: 10.1089/jir.2022.0098

    PMID: 36179036

    		 Sample: fecal
  12. Infectious Agents and Cancer (2023) IF: 3.698
    Inhibitory effect and mechanism of Lactobacillus crispatus on cervical precancerous cells Ect1/E6E7 and screening of early warning factors

    DOI: 10.1186/s13027-023-00483-1
    Sample: Ect1/E6E7 cell
  13. PLoS One (2022) IF: 3.752
    Exploring alternative cytokines as potential biomarkers for latent tuberculosis infection in pregnant women

    DOI: 10.1371/journal.pone.0270552

    PMID: 35802700

    		 Sample: plasma
  14. PLoS Neglected Tropical Diseases (2016) IF: 3.948
    Clonorchis sinensis Co-infection Could Affect the Disease State and Treatment Response of HBV Patients

    DOI: 10.1371/journal.pntd.0004806
    Sample: Serum,Cell culture supernatant
  15. POULTRY SCIENCE (2019) IF: 2.027
    The effect of different dietary ratios of arginine, methionine, and lysine on the performance, carcass traits, and immune status of turkeys

    DOI: 10.1016/j.psj.2019.10.008

    PMID: 32036960

    		 Sample: Plasma
  16. APMIS (2019) IF: 2.225
    Autoantibodies to a specific peptide epitope of human Hsp60 (ATVLA) with homology to Helicobacter pylori HspB in H. pylori‐infected patients

    DOI: 10.1111/apm.12925
    Sample: Cell culture supernatant
  17. Applied Sciences-Basel (2021) IF: 2.679
    Biochemical Mapping of the Inflamed Human Dental Pulp

    DOI: 10.3390/app112110395
    Sample: dental pulp lysates
  18. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH (2020) IF: 1.287
    Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells:

    DOI: 10.1177/0300060520930853
    Sample: Cell culture supernatant
  19. Journal of Obsessive-Compulsive and Related Disorders (2021) IF: 1.677
    TGF-β1, neopterin, tetrahydrobiopterin, and nitric oxide levels in pediatric obsessive–compulsive disorder

    DOI: 10.1016/j.jocrd.2021.100649
    Sample: Serum
  20. MEDICINE (2017) IF: 1.804
    Ketamine, as adjuvant analgesics for patients with refractory cancer pain, does affect IL-2/IFN-γ expression of T cells in vitro?: A prospective, randomized, double-blind study

    DOI: 10.1097/MD.0000000000006639
    Sample: T cell
  21. Journal of Advanced Pharmaceutical Technology & Research (2020)
    The enhancement apoptosis of osteosarcoma mesenchymal stem cells co-cultivation with peripheral blood mononuclear cells sensitized by secretome and granulocyte macrophage colony-stimulating factor

    DOI: 10.4103/japtr.JAPTR_52_20
    Sample: PBMCs
  22. Meta Gene (2019)
    Interleukin-2 serum level, genetic polymorphism (rs2069763), anti-rubella antibody and risk of multiple sclerosis among Iraqi patients

    DOI: 10.1016/j.mgene.2019.100642
    Sample: Serum
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