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Human IL-23(Interleukin 23) ELISA Kit

Citations(10)Uniprot : Q9NPF7,P29460

Cat.No.:E-EL-H0107
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	39.06-2500 pg/mL
Sensitivity	23.44 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human IL-23 in samples. No significant cross-reactivity or interference between Human IL-23 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human IL-23 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-23. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-23. You can calculate the concentration of Human IL-23 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-23 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-23 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	136.57	343.26	1072.26	129.52	356.17	1144.30
Standard deviation	7.20	15.55	35.49	8.48	20.02	54.47
CV (%)	5.27	4.53	3.31	6.55	5.62	4.76

Recovery

The recovery of Human IL-23 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-100	94
EDTA plasma (n=8)	94-105	99
Cell culture media (n=8)	87-100	93

Linearity

Samples were spiked with high concentrations of Human IL-23 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q9NPF7 P29460
Synonyms	IL-23A, IL23P19, P19, SGRF
Research Area	Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF INVESTIGATIVE DERMATOLOGY (2021) IF: 8.551
Cyclin-Dependent Kinase 7 Promotes Th17/Th1 Cell Differentiation in Psoriasis by Modulating Glycolytic Metabolism

DOI: 10.1016/j.jid.2021.04.018

PMID: 34004188
Sample: Serum
JOURNAL OF IMMUNOLOGY (2022) IF: 5.422
Fungal Patterns Induce Cytokine Expression through Fluxes of Metabolic Intermediates That Support Glycolysis and Oxidative Phosphorylation

DOI: 10.4049/jimmunol.2100666

PMID: 35688467
Sample: human monocyte-derived dendritic cells (DCs)
PLoS Neglected Tropical Diseases (2021) IF: 4.411
Anaphylatoxins orchestrate Th17 response via interactions between CD16+ monocytes and pleural mesothelial cells in tuberculous pleural effusion

DOI: 10.1371/journal.pntd.0009508

PMID: 34237073
Sample: pleural fluid
INTERNATIONAL IMMUNOPHARMACOLOGY (2019) IF: 4.932
Arsenic trioxide improves Treg and Th17 balance by modulating STAT3 in treatment-naïve rheumatoid arthritis patients

DOI: 10.1016/j.intimp.2019.05.001

Sample: PBMCs cells
Pharmacological Reports (2021) IF: 3.027
The association of the quality of sleep with proinflammatory cytokine profile in inflammatory bowel disease patients

DOI: 10.1007/s43440-021-00333-0

PMID: 34694623
Sample: serum
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2020) IF: 2.352
A case of asthma with Behcet’s disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions

DOI: 10.1080/08923973.2020.1789656

Sample: Serum
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022) IF: 2.607
Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Alleviate Psoriasis-like Skin Inflammation

DOI: 10.1089/jir.2021.0146

PMID: 35041513
Sample: HaCaT cell
ANDROLOGIA (2022) IF: 2.775
Relationship of interleukin-23 with matrix metalloproteinase-9, pentraxin-3, sleep quality and prostate size in benign prostatic hyperplasia

DOI: 10.1111/and.14387

PMID: 35092070
Sample: serum
JOURNAL OF INVESTIGATIVE SURGERY (2020) IF: 1.685
New Findings on Autoimmune Etiology of Idiopathic Granulomatous Mastitis: Serum IL-17, IL-22 and IL-23 Levels of Patients

DOI: 10.1080/08941939.2020.1725190

Sample: serum
Annals of Medicine and Surgery (2022)
The roles of Single Nucleotide Polymorphism (SNP) Endoplasmic Reticulum Aminopeptidase 1 (ERAP 1) gene in axial spondyloarthritis Indonesian adults

DOI: 10.1016/j.amsu.2022.103675

PMID: 35638067
Sample: peripheral blood mononuclear cell (PBMC)

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