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Human MMP-2(Matrix Metalloproteinase 2) ELISA Kit

Citations(19)Uniprot : P08253

Cat.No.:E-EL-H1445
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.78-50 ng/mL
Sensitivity	0.47 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human MMP-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-2. You can calculate the concentration of Human MMP-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MMP-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MMP-2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.40	5.30	21.80	2.40	5.10	20.80
Standard deviation	0.10	0.30	0.90	0.20	0.20	0.70
CV (%)	4.17	5.66	4.13	8.33	3.92	3.37

Recovery

The recovery of Human MMP-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-100	92
EDTA plasma (n=8)	87-99	92
Cell culture media (n=8)	88-100	93

Linearity

Samples were spiked with high concentrations of Human MMP-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P08253
Synonyms	MMP2, CLG4, CLG4A, MMP-II, MONA, TBE-1, Gelatinase A
Research Area	Cancer, Cell Biology, Cardiovascular, Metabolism

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF HEPATOLOGY (2019) IF: 18.946
MMP2/MMP9-mediated CD100 shedding is crucial for inducing intrahepatic anti-HBV CD8 T cell responses and HBV clearance

DOI: 10.1016/j.jhep.2019.05.013

PMID: 31173811
Sample: Serum
Nature Communications (2021) IF: 14.919
Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers

DOI: 10.1038/s41467-021-23789-3

PMID: 34078895
Sample: plasma
BIOMEDICINE & PHARMACOTHERAPY (2023) IF: 7.419
Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis

DOI: 10.1016/j.biopha.2023.114289

Sample: cardiac fibroblast
Marine Drugs (2023) IF: 6.085
Mechanisms of Antitumor Invasion and Metastasis of the Marine Fungal Derivative Epi-Aszonalenin A in HT1080 Cells

DOI: 10.3390/md21030156

Sample: HT1080 cell
Cells (2021) IF: 6.6
The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases

DOI: 10.3390/cells10123506

Sample: cardiac fibroblast
INDUSTRIAL CROPS AND PRODUCTS (2022) IF: 5.645
Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction

DOI: 10.1016/j.indcrop.2022.115164

Sample: Normal human dermal fibroblasts (NHDF)
INTERNATIONAL JOURNAL OF ONCOLOGY (2014) IF: 5.65
Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo

DOI: 10.3892/ijo.2014.2645

Sample: Cell culture supernatant
Inorganic Chemistry Frontiers (2017) IF: 4.036
Anthraquinone-bridged diruthenium(II) complexes inhibit migration and invasion of human hepatocarcinoma MHCC97-H cells

DOI: 10.1039/C7QI00149E

Sample: Cell culture supernatant
Spine Journal (2022) IF: 4.166
Extracellular vesicle and soluble fractions of adipose tissue-derived mesenchymal stem cells secretome induce inflammatory cytokines modulation in an in vitro model of discogenic pain

DOI: 10.1016/j.spinee.2022.01.012

PMID: 35121152
Sample: human adipose-derived mesenchymal stem cells (HAdM
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways

DOI: 10.1016/j.jep.2021.114791

PMID: 34737112
Sample: MH7A cell
Biomolecules (2019) IF: 4.694
MMP2 Polymorphism Affects Plasma Matrix Metalloproteinase (MMP)-2 Levels, and Correlates with the Decline in Lung Function in Hypersensitivity Pneumonitis Positive to Autoantibodies Patients

DOI: 10.3390/biom9100574

Sample: plasma
MOLECULES (2019) IF: 3.06
Ampelopsin E Reduces the Invasiveness of the Triple Negative Breast Cancer Cell Line, MDA-MB-231

DOI: 10.3390/molecules24142619

Sample: MDA-MB-231 cells
PLACENTA (2022) IF: 3.481
Fenofibrate mediated activation of PPARα negatively regulates trophoblast invasion

DOI: 10.1016/j.placenta.2022.05.015

PMID: 35803128
Sample: HTR8/SVneo cell
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY (2021) IF: 2.568
Serum fibrosis markers could aid in the prediction of factor for successful oral antiviral treatment in hepatitis C

DOI: 10.1097/MEG.0000000000002083

PMID: 33560684
Sample: Blood
ANNALS OF VASCULAR SURGERY (2022) IF: 1.607
Correlation between proteolytic activity and abdominal aortic aneurysm wall morphology with intraluminal thrombus volume

DOI: 10.1016/j.avsg.2022.05.039

PMID: 35779804
Sample: Abdominal Aortic Aneurysm (AAA) Wall
BIOLOGIA (2022) IF: 1.653
UPLC-ESI-QTOF-MS/MS and 1H-NMR identification of alkaloids in potent fraction of Catharanthus roseus leaves inhibits migration and invasion of MDA-MB-231 cells

DOI: 10.1007/s11756-022-01185-6

Sample: MDA-MB-231 cell
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2017) IF: 1.685
Expression and Significance of MMPs in Synovial Fluid, Serum and PBMC Culture Supernatant Stimulated by LPS in Osteoarthritis Patients With or Without Diabetes

DOI: 10.1055/s-0043-122223

PMID: 29270923
Sample: Serum,Synovia
BIOLOGIA (2020) IF: 0.8110
Catharanthus roseus L. extract downregulates the expression profile of motility-related genes in highly invasive human breast cancer cell line MDA-MB-231

DOI: 10.2478/s11756-020-00641-5

Sample: MDA-MB-231 cell
JOURNAL OF CLINICAL ULTRASOUND (2021) IF: 0.91
Clinical value of 2D speckle tracking imaging in evaluating the effect of percutaneous intramyocardial septal radiofrequency ablation in patients with hypertrophic obstructive cardiomyopathy

DOI: 10.1002/jcu.22989

PMID: 33675543
Sample: Plasma

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