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Human MMP-3(Matrix Metalloproteinase 3) ELISA Kit

Citations(20)Uniprot : P08254

Cat.No.:E-EL-H1446
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human MMP-3 in samples. No significant cross-reactivity or interference between Human MMP-3 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human MMP-3 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-3. You can calculate the concentration of Human MMP-3 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MMP-3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MMP-3 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.30	4.50	0.50	1.40	4.80
Standard deviation	0.03	0.07	0.24	0.03	0.07	0.14
CV (%)	6.00	5.38	5.33	6.00	5.00	2.92

Recovery

The recovery of Human MMP-3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-103	96
EDTA plasma (n=8)	94-106	101
Cell culture media (n=8)	88-103	95

Linearity

Samples were spiked with high concentrations of Human MMP-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P08254
Synonyms	MMP3, CHDS6, SL-1, STMY, STMY1, STR1, Stromelysin-1
Research Area	Cancer, Cell Biology, Cardiovascular, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Nature Communications (2021) IF: 14.919
Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers

DOI: 10.1038/s41467-021-23789-3

PMID: 34078895
Sample: plasma
Science Signaling (2016) IF: 7.359
Quantitative phosphoproteomic analysis identifies the critical role of JNK1 in neuroinflammation induced by Japanese encephalitis virus

DOI: 10.1126/scisignal.aaf5132

Sample: Human glioma cell line U251
Antioxidants (2022) IF: 7.675
Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from Sea buckthorn on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts

DOI: 10.3390/antiox11101900

PMID: 36290623
Sample: HSFs
Marine Drugs (2021) IF: 5.118
Heptapeptide Isolated from Isochrysis zhanjiangensis Exhibited Anti-Photoaging Potential via MAPK/AP-1/MMP Pathway and Anti-Apoptosis in UVB-Irradiated HaCaT Cells

DOI: 10.3390/md19110626

PMID: 34822497
Sample: HaCaT cells
CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
A novel glyceroglycolipid from brown algae Ishige okamurae improve photoaging and counteract inflammation in UVB-induced HaCaT cells

DOI: 10.1016/j.cbi.2021.109737

PMID: 34740599
Sample: HaCaT cells
Journal of Functional Foods (2022) IF: 5.223
High fiber multigrain supplementation improved disease activity score, circulating inflammatory and oxidative stress biomarkers in rheumatoid arthritis (RA) patients: A randomized human clinical trial

DOI: 10.1016/j.jff.2022.105392

Sample: blood
OSTEOARTHRITIS AND CARTILAGE (2018) IF: 5.454
Associations between serum S100A8/S100A9 and knee symptoms, joint structures and cartilage enzymes in patients with knee osteoarthritis

DOI: 10.1016/j.joca.2018.08.020

PMID: 30240939
Sample: Serum
Frontiers in Pharmacology (2022) IF: 5.988
Aconiti lateralis radix praeparata total alkaloids exert anti-RA effects by regulating NF-κB and JAK/STAT signaling pathways and promoting apoptosis.

DOI: 10.3389/fphar.2022.980229

Sample: MH7A cell
Spine Journal (2022) IF: 4.166
Extracellular vesicle and soluble fractions of adipose tissue-derived mesenchymal stem cells secretome induce inflammatory cytokines modulation in an in vitro model of discogenic pain

DOI: 10.1016/j.spinee.2022.01.012

PMID: 35121152
Sample: human adipose-derived mesenchymal stem cells (HAdM
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways

DOI: 10.1016/j.jep.2021.114791

PMID: 34737112
Sample: MH7A cell
OSTEOARTHRITIS AND CARTILAGE (2017) IF: 4.742
Associations between serum ghrelin and knee symptoms, joint structures and cartilage or bone biomarkers in patients with knee osteoarthritis

DOI: 10.1016/j.joca.2017.05.015

Sample: Serum
PPAR Research (2019) IF: 4.964
Arsenic Trioxide in Synergy with Vitamin D Rescues the Defective VDR-PPAR- Functional Module of Autophagy in Rheumatoid Arthritis

DOI: 10.1155/2019/6403504

Sample: rheumatoid arthritis (RA) fibroblast-like synovioc
Journal of Functional Foods (2020) IF: 3.701
Trehalose against UVB-induced skin photoaging by suppressing MMP expression and enhancing procollagen I synthesis in HaCaT cells

DOI: 10.1016/j.jff.2020.104198

Sample: HaCaT cell
CLINICAL RHEUMATOLOGY (2019) IF: 2.293
Associations between serum IL-8 and knee symptoms, joint structures, and cartilage or bone biomarkers in patients with knee osteoarthritis

DOI: 10.1007/s10067-019-04718-8

PMID: 31377918
Sample: Serum
INTERNATIONAL DENTAL JOURNAL (2022) IF: 2.607
Efficacy of Proanthocyanidins in Nonsurgical Periodontal Therapy

DOI: 10.1016/j.identj.2022.08.006

PMID: 36167610
Sample: saliva samples
RHEUMATOLOGY INTERNATIONAL (2022) IF: 2.631
Assessment of serum and synovial fluid MMP-3 and MPO as biomarkers for psoriatic arthritis and their relation to disease activity indices

DOI: 10.1007/s00296-022-05159-4

PMID: 35708757
Sample: plasma
Applied Sciences-Basel (2022) IF: 2.838
Effectiveness of a Cucumber Extract Supplement on Articular Pain in Patients with Knee Osteoarthritis: A Randomized Double-Blind Controlled Clinical Trial

DOI: 10.3390/app13010485

Sample: plasma
Iranian Journal of Basic Medical Sciences (2018) IF: 1.514
Effects of insulin-like growth factor-induced Wharton jelly mesenchymal stem cells toward chondrogenesis in an osteoarthritis model

DOI: 10.22038/IJBMS.2018.28205.6840

Sample: Serum
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2017) IF: 1.685
Expression and Significance of MMPs in Synovial Fluid, Serum and PBMC Culture Supernatant Stimulated by LPS in Osteoarthritis Patients With or Without Diabetes

DOI: 10.1055/s-0043-122223

PMID: 29270923
Sample: Serum,Synovia
International Journal of Rheumatic Diseases (2020) IF: 1.98
Associations of serum citrate levels with knee structural changes and cartilage enzymes in patients with knee osteoarthritis

DOI: 10.1111/1756-185X.13787

Sample: serum

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