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Human PON1(Paraoxonase 1) ELISA Kit

Citations(7)Uniprot : P27169

Cat.No.:E-EL-H2298
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human PON1 in samples. No significant cross-reactivity or interference between Human PON1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human PON1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PON1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PON1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PON1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PON1. You can calculate the concentration of Human PON1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PON1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PON1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.46	1.53	4.72	0.45	1.47	4.63
Standard deviation	0.03	0.06	0.26	0.03	0.06	0.19
CV (%)	6.52	3.92	5.51	6.67	4.08	4.10

Recovery

The recovery of Human PON1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-105	98
EDTA plasma (n=8)	86-99	91
Cell culture media (n=8)	95-108	100

Linearity

Samples were spiked with high concentrations of Human PON1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P27169
Synonyms	ESA, MVCD5, Aromatic Esterase 1, Serum Aryldialkylphosphatase 1
Research Area	Cancer, Cell Biology, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Antioxidants (2022) IF: 6.313
Effect of Low-Dose Radiotherapy on the Circulating Levels of Paraoxonase-1-Related Variables and Markers of Inflammation in Patients with COVID-19 Pneumonia

DOI: 10.3390/antiox11061184

PMID: 35740079
Sample: serum
Antioxidants (2021) IF: 6.313
Clinical Performance of Paraoxonase-1-Related Variables and Novel Markers of Inflammation in Coronavirus Disease-19. A Machine Learning Approach

DOI: 10.3390/antiox10060991

PMID: 34205807
Sample: serum
BMC Cardiovascular Disorders (2022) IF: 2.174
Associations between myeloperoxidase and paraoxonase-1 and type 2 diabetes in patients with ischemic heart disease

DOI: 10.1186/s12872-022-02928-8

Sample: serum
PLoS One (2018) IF: 2.766
Impact of the 24-h ultramarathon race on homocysteine, oxidized low-density lipoprotein, and paraoxonase 1 levels in professional runners

DOI: 10.1371/journal.pone.0192392

Sample: serum
ANNALS OF VASCULAR SURGERY (2019) IF: 1.179
Paraoxonase-1 and Symptomatic Status in Carotid Artery Disease

DOI: 10.1016/j.avsg.2019.07.020

PMID: 31626928
Sample: Serum
MEDICINE (2022) IF: 1.817
Paraoxonase 1 and atrial fibrillation: Is there a relationship?

DOI: 10.1097/MD.0000000000031553

Sample: serum
Meta Gene (2018)
Association of cholesteryl ester transferyl protein gene (G277A) polymorphism and risk prediction of type 2 diabetes mellitus: A case control study of Northern Indian population

DOI: 10.1016/j.mgene.2018.06.005

Sample: Serum

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