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Human SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

Citations(13)Uniprot : P00441

Cat.No.:E-EL-H1113
Reactivity: Human

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	62.50-4000 pg/mL
Sensitivity	37.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of SOD1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD1. During the reaction, SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SOD1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SOD1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	209.30	367.40	1835.40	196.20	331.30	1975.00
Standard deviation	13.20	14.70	55.10	12.80	16.60	75.10
CV (%)	6.31	4.00	3.00	6.52	5.01	3.80

Recovery

The recovery of SOD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-108	102
EDTA plasma(n=8)	88-101	94
Cell culture media(n=8)	91-105	97

Linearity

Samples were spiked with high concentrations of Human SOD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P00441
Synonyms	ALS, ALS1, IPOA, Superoxide dismutase [Cu-Zn]
Research Area	Cancer, Cell Biology, Metabolism, Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

Antioxidants (2021) IF: 6.313
Antioxidant Effect of a Probiotic Product on a Model of Oxidative Stress Induced by High-Intensity and Duration Physical Exercise

DOI: 10.3390/antiox10020323

PMID: 33671691
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
4-thiazolidinone-based derivatives rosiglitazone and pioglitazone affect the expression of antioxidant enzymes in different human cell lines

DOI: 10.1016/j.biopha.2021.111684

PMID: 34243632
Sample: Cell culture supernatant
INDOOR AIR (2022) IF: 6.554
Organic pollutant exposure and health effects of cooking emissions on kitchen staff in food services

DOI: 10.1111/ina.13093

PMID: 36040287
Sample: serum
Frontiers in Cellular Neuroscience (2022) IF: 5.505
14,15-Epoxyeicosatrienoic Acid Protect Against Glucose Deprivation and Reperfusion-Induced Cerebral Microvascular Endothelial Cells Injury by Modulating Mitochondrial Autophagy via SIRT1/FOXO3a Signaling Pathway and TSPO Protein

DOI: 10.3389/fncel.2022.888836

PMID: 35558879
Sample: HCMECs
Nutrients (2021) IF: 5.719
Effects of the Consumption of Low-Fat Cooked Ham with Reduced Salt Enriched with Antioxidants on the Improvement of Cardiovascular Health: A Randomized Clinical Trial

DOI: 10.3390/nu13051480

PMID: 33925704
Sample: Serum
Toxins (2021) IF: 4.546
Biomarkers of Uremic Cardiotoxicity

DOI: 10.3390/toxins13090639

PMID: 34564643
Sample: serum
APPLIED CLAY SCIENCE (2020) IF: 4.605
Synergistic anti-cancer activity of etoposide drug loaded calcium aluminium layered double hydroxide nanoconjugate for possible application in non small cell lung carcinoma

DOI: 10.1016/j.clay.2020.105496

Sample: A549 cell
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2018) IF: 3.974
Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oil

DOI: 10.1016/j.ecoenv.2018.04.006

Sample: Tissue homogenate
Dose-Response (2022) IF: 2.623
Correlation of Ionized Magnesium with the Parameters of Oxidative Stress as Potential Biomarkers in Patients with Anxiety and Depression: A Pilot Study:

DOI: 10.1177/15593258221116741

Sample: serum
IUBMB LIFE (2014) IF: 2.755
2,3,5,4′‐tetrahydroxystilbene‐2‐O‐β‐d‐glucoside protects human umbilical vein endothelial cells against lysophosphatidylcholine‐induced apoptosis by upregulating superoxide dismutase and glutathione peroxidase

DOI: 10.1002/iub.1321

Sample: Cell lysate
Indian Heart Journal (2017)
The role of polysaccharide peptide of Ganoderma lucidum as a potent antioxidant against atherosclerosis in high risk and stable angina patients

DOI: 10.1016/j.ihj.2017.12.007

Sample: plasma
SAGE Open Medicine (2021)
Deficiency of antioxidants and increased oxidative stress in COVID-19 patients: A cross-sectional comparative study in Jigawa, Northwestern Nigeria:

DOI: 10.1177/2050312121991246

Sample: serum
International Journal of Reproductive Biomedicine (2021)
Association between a marker of sperm DNA damage and sperm indices in infertile males in Benin City, Nigeria: A cross-sectional study

DOI: 10.18502/ijrm.v19i2.8472

Sample: Semen sample

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Reviews/Q&A

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Reviews
Q&A

N****mSubmitted [ Apr 05 2023 ]

Asked: EL- H1113 human SOD 1 EL-0060 MDA EL-H5410 human GPX EL-H0742 NOS n Please let me know reagents 1. Does each of above kit has different biotinylated detection AB 2. Conjugate HRP 3. Substrate solution 4. Stop solution 5. Buffer solution in each kit 6. Would like to know like the composition of 1,2 3, 4,5

adminSubmitted [ Apr 05 2023 ]

Answered: For these kits, there are differences in biotinylated detection AB, Conjugate HRP, Substrate solution and Buffer solution in each kit. The composition of Stop solution is the same, so in theory, other reagents are recommended not to be mixed except Stop solution. As the reagent composition of the kit is confidential, we are sorry that we cannot tell you. We know that there are a lot of Buffer solutions in the kit. If you gets confused, please tell us the lot number information of the kit, the name of the Buffer solution and the LOT number information, so that we can help to distinguish.

N****mSubmitted [ Dec 28 2022 ]

Asked: If all Elsa kits have same composition for dilute ent of blood samples

adminSubmitted [ Dec 28 2022 ]

Answered: The components of the standard & sample diluent in the ELISA kits of different target proteins may be different. Therefore, to ensure the accuracy of the test results, the standard & sample diluent from other target protein kits should be avoided during the experiment

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