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MG(Malachite Green) ELISA Kit

  • Cat.No.:E-FS-E013

  • Sample type: Muscle
  • Reaction mode: 25℃; 30min,30min,15min
  • Reactivity: Universal

To Purchase E-FS-E013

Size:
  • 96T*3
  • 96T
Price: $1200
Qty:

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Specifications

Intended use The kit is a competitive enzyme immunoassay for the quantitative analysis of Malachite Green in Muscle. Please contact your area sales manager for the applicability if other sample need to be tested.
Incubation time 75min
LOD (Detection limit) Muscle---0.1ppb
Sample preparation For mentioned matrices fast and efficient extraction methods are included in the kit description. If you need to test other samples, Please contact your area sales manager.
Detection range See the manual for details
Evaluation Microtiter plate spectrophotometer (450 nm/630nm)
Storage 2-8℃,avoid freeze
Expiration date The kit have an expiry date of 1 year from the production date.

Background

Malachite green has been widely used to prevent and treat saprolegniasis, branchial mildew, and melon worm disease in various aquatic animals. However, malachite green and its metabolite colorless malachite green will produce high residues in aquatic products, which can easily cause carcinogenic, teratogenic and mutagenic side effects after human consumption.

Compared with traditional methods,the ElabscienceMalachite Green kit is simple, fast, and sensitive, and is suitable for the detection of large quantities of samples.

Elabsciencefood safety kits are used to detect hormone residues, mycotoxins, toxic and harmful substances and antibiotic residues in feed, food and other sample. It can be widely used in food and drug Administration, health departments, various colleges, scientific research units, agricultural departments, animal husbandry and veterinarians, breeding farms, slaughterhouses, food and meat products deep processing enterprises, inspection and quarantine departments, etc. It can meet the high requirements of consumers for food quality and the scientific researchers to obtain efficient experimental data. The kit is manufactured in accordance with the international quality standard ISO9001.

Kits components

Citations

Reviews/Q&A

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  • Q&A
Q

M***lSubmitted [ Nov 18 2022 ]

Asked: Is this antibody monoclonal or policlonal?

Reply

A

adminSubmitted [ Nov 18 2022 ]

Answered: Hello Micol, Thanks for your question. The antibody adopted in the Malachite green ELISA kit is in polyclonal. This kit uses Competitive-ELISA as the method. It can detect Malachite Green (MG) in samples, such as muscle, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, MG in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-MG antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of MG. The concentration of MG in the samples can be calculated by comparing the OD of the samples to the standard curve. Please let me know if i can be of any further assistance.

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