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Mouse αFP(Alpha-Fetoprotein) ELISA Kit

Citations(6)Uniprot : P02772

Cat.No.:E-EL-M2405
Reactivity: Mouse

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse αFP in samples. No significant cross-reactivity or interference between Mouse αFP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse αFP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse αFP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse αFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse αFP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse αFP. You can calculate the concentration of Mouse αFP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse αFP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse αFP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.30	4.60	0.50	1.20	5.00
Standard deviation	0.03	0.07	0.16	0.03	0.07	0.23
CV (%)	6.00	5.38	3.48	6.00	5.83	4.60

Recovery

The recovery of Mouse αFP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-101	96
EDTA plasma (n=8)	95-108	101
Cell culture media (n=8)	92-105	97

Linearity

Samples were spiked with high concentrations of Mouse αFP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P02772
Synonyms	AFP, FETA, HPAFP, Alpha-fetoglobulin, oralpha fetal protein
Research Area	Cancer, Cardiovascular, Developmental biology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

CARBOHYDRATE POLYMERS (2022) IF: 10.723
Hepatoma-targeting and reactive oxygen species-responsive chitosan-based polymeric micelles for delivery of celastrol

DOI: 10.1016/j.carbpol.2022.120439

Sample: serum,H22 cell-Xenograft
JOURNAL OF PATHOLOGY (2021) IF: 7.996
Disordered farnesoid X receptor signaling is associated with liver carcinogenesis in Abcb11-deficient mice

DOI: 10.1002/path.5780

PMID: 34410012
Sample: serum
INTERNATIONAL JOURNAL OF PHARMACEUTICS (2022) IF: 6.51
Norcantharidin liposome emulsion hybrid delivery system enhances PD-1/PD-L1 immunotherapy by agonizing the non-canonical NF-κB pathway

DOI: 10.1016/j.ijpharm.2022.122361
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31
Oestradiol promotes the intrahepatic bile duct development of C57BL/6CrSlc mice during embryonic period via Notch signalling pathway

DOI: 10.1111/jcmm.16888

PMID: 34498380
Sample: hepatoblasts
Genes (2022) IF: 4.141
In Silico and In Vivo Evaluation of microRNA-181c-5p’s Role in Hepatocellular Carcinoma

DOI: 10.3390/genes13122343
CURRENT GENE THERAPY (2019) IF: 2.218
Effect of Tumor Suppressor MiR-34a Loaded on ZSM-5 Nanozeolite in Hepatocellular Carcinoma: In Vitro and In Vivo Approach

DOI: 10.2174/1566523219666191108103739

Sample: liver

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