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Mouse ADP/Acrp30(Adiponectin) ELISA Kit

Citations(20)Uniprot : Q60994

Cat.No.:E-EL-M0002
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse ADP/Acrp30 in samples. No significant cross-reactivity or interference between Mouse ADP/Acrp30 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse ADP/Acrp30 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ADP/Acrp30. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ADP/Acrp30. You can calculate the concentration of Mouse ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse ADP/Acrp30 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse ADP/Acrp30 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	49.92	135.68	423.36	53.10	140.06	384.77
Standard deviation	2.56	7.18	19.86	3.61	7.26	20.78
CV (%)	5.13	5.29	4.69	6.80	5.18	5.40

Recovery

The recovery of Mouse ADP/Acrp30 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-100	95
EDTA plasma (n=8)	93-111	101
Cell culture media (n=8)	86-99	93

Linearity

Samples were spiked with high concentrations of Mouse ADP/Acrp30 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q60994
Synonyms	Acrp30, GBP28, ACDC, APM1, ADPN, AdipoQ, ADIPQTL1
Research Area	Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

FREE RADICAL BIOLOGY AND MEDICINE (2018) IF: 6.02
Procyanidin B2 ameliorates free fatty acids-induced hepatic steatosis through regulating TFEB-mediated lysosomal pathway and redox state

DOI: 10.1016/j.freeradbiomed.2018.08.024

Sample: serum
Antioxidants (2022) IF: 6.313
Ramulus Mori (Sangzhi) Alkaloids Alleviate High-Fat Diet-Induced Obesity and Nonalcoholic Fatty Liver Disease in Mice

DOI: 10.3390/antiox11050905

PMID: 35624769
Sample: 3T3-L1 cell
INTERNATIONAL JOURNAL OF PHARMACEUTICS (2023) IF: 6.51
Fat fighting liraglutide based nano-formulation to reverse obesity: Design, development and animal trials

DOI: 10.1016/j.ijpharm.2023.122585

Sample: serum
LIFE SCIENCES (2023) IF: 6.78
Immuno-metabolic effect of pancreastatin inhibitor PSTi8 in diet induced obese mice: In vitro and in vivo findings

DOI: 10.1016/j.lfs.2023.121415

Sample: serum
PHARMACOLOGICAL RESEARCH (2020) IF: 5.893
Cryptotanshinone alleviates chemotherapy-induced colitis in mice with colon cancer via regulating fecal-bacteria-related lipid metabolism

DOI: 10.1016/j.phrs.2020.105232

PMID: 33027716
Sample: Serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924
Anti-Obesity and Anti-Hyperglycemic Effects of Meretrix lusoria Protamex Hydrolysate in ob/ob Mice

DOI: 10.3390/ijms23074015

PMID: 35409375
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells

DOI: 10.1016/j.biopha.2020.110684

Sample: serum
EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
Pancreastatin inhibitor PSTi8 attenuates hyperinsulinemia induced obesity and inflammation mediated insulin resistance via MAPK/NOX3-JNK pathway

DOI: 10.1016/j.ejphar.2019.172723

PMID: 31586632
Sample: Plasma
EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 3.263
Pancreastatin inhibitor PSTi8 protects the obesity associated skeletal muscle insulin resistance in diet induced streptozotocin-treated diabetic mice

DOI: 10.1016/j.ejphar.2020.173204

Sample: plasma
Journal of Diabetes (2019) IF: 3.298
Renoprotective effects of brown adipose tissue activation in diabetic mice

DOI: 10.1111/1753-0407.12938

PMID: 31020790
Sample: Serum,Urine
TOXICOLOGY AND APPLIED PHARMACOLOGY (2020) IF: 3.347
Role of TRPV1/TRPV3 channels in olanzapine-induced metabolic alteration: Possible involvement in hypothalamic energy-sensing, appetite regulation, inflammation and mesolimbic pathway

DOI: 10.1016/j.taap.2020.115124

PMID: 32652086
Sample: Serum
TOXICOLOGY AND APPLIED PHARMACOLOGY (2019) IF: 3.585
Modeling of antipsychotic-induced metabolic alterations in mice: An experimental approach precluding psychosis as a predisposing factor

DOI: 10.1016/j.taap.2019.114643

PMID: 31254565
Sample: Serum
LIFE SCIENCES (2020) IF: 3.647
Berberine attenuated olanzapine-induced metabolic alterations in mice: Targeting transient receptor potential vanilloid type 1 and 3 channels

DOI: 10.1016/j.lfs.2020.117442

Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
Pancreastatin inhibitor activates AMPK pathway via GRP78 and ameliorates dexamethasone induced fatty liver disease in C57BL/6 mice

DOI: 10.1016/j.biopha.2019.108959

Sample: plasma
PEPTIDES (2022) IF: 3.867
Pancreastatin inhibitor PSTi8 balances energy homeostasis by attenuating adipose tissue inflammation in high fat diet fed mice

DOI: 10.1016/j.peptides.2022.170902

Sample: serum
JOURNAL OF MEDICINAL FOOD (2019) IF: 2.02
β-Sitosterol Shows Potential to Protect Against the Development of High-Fructose Diet-Induced Metabolic Dysfunction in Female Rats

DOI: 10.1089/jmf.2019.0120

Sample: plasma
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY (2020) IF: 2.056
Virgin Grape Seed Oil Alleviates Insulin Resistance and Energy Metabolism Disorder in Mice Fed a High‐Fat Diet

DOI: 10.1002/ejlt.201900158

Sample: Serum
3 Biotech (2021) IF: 2.406
A bioactive fraction of Pterocarpus santalinus inhibits adipogenesis and inflammation in 3T3-L1 cells via modulation of PPAR-γ/SREBP-1c and TNF-α/IL-6

DOI: 10.1007/s13205-021-02771-2

PMID: 33968577
Sample: Cell sample
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 2.575
Astragalin, (3-O-glucoside of kaempferol), isolated from Moringa oleifera leaves modulates leptin, adiponectin secretion and inhibits adipogenesis in 3T3-L1 adipocytes

DOI: 10.1080/13813455.2020.1740742

Sample: 3T3-L1 cell
Toxicology Reports (2022)
Gallic acid reverses ovarian disturbances in mice with letrozole-induced PCOS via modulating Adipo R1 expression

DOI: 10.1016/j.toxrep.2022.10.009

Sample: plasma

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