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Mouse AST(Aspartate Aminotransferase) ELISA Kit

Citations(12)Uniprot : P05201

Cat.No.:E-EL-M0160
Reactivity: Mouse

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96T
48T
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse AST in samples. No significant cross-reactivity or interference between Mouse AST and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse AST concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse AST. You can calculate the concentration of Mouse AST in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse AST were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse AST were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.49	0.85	4.61	0.46	0.79	4.49
Standard deviation	0.03	0.04	0.17	0.02	0.03	0.23
CV (%)	6.12	4.71	3.69	4.35	3.80	5.12

Recovery

The recovery of Mouse AST spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-108	101
EDTA plasma (n=8)	90-102	97
Cell culture media (n=8)	88-101	95

Linearity

Samples were spiked with high concentrations of Mouse AST and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05201
Synonyms	SGOT, serum glutamic oxaloacetic transaminase, GOT1, AspAT, ASAT, AAT
Research Area	Cancer, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

mBio (2021) IF: 7.867
Ileal Bile Acid Transporter Inhibitor Improves Hepatic Steatosis by Ameliorating Gut Microbiota Dysbiosis in NAFLD Model Mice | mBio

DOI: 10.1128/mBio.01155-21

Sample: serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Apolipoprotein A-IV Has Bi-Functional Actions in Alcoholic Hepatitis by Regulating Hepatocyte Injury and Immune Cell Infiltration

DOI: 10.3390/ijms24010670

Sample: AML-12 cell
Viruses-Basel (2021) IF: 5.048
Favipiravir Inhibits Mayaro Virus Infection in Mice

DOI: 10.3390/v13112213

PMID: 34835018
Sample: serum
JOURNAL OF INORGANIC BIOCHEMISTRY (2021) IF: 4.155
In vitro and in vivoactivity of series of cationic dinuclearPt(II) complexes

DOI: 10.1016/j.jinorgbio.2021.111619

PMID: 34597886
Sample: serum
Journal of Functional Foods (2021) IF: 4.451
Heracleum dissectum Ledeb. ethanol extract attenuates metabolic syndrome symptoms in high-fat diet-induced obese mice by activating adiponectin/AMPK signaling

DOI: 10.1016/j.jff.2021.104581

Sample: blood
European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
MiR-181-5p protects mice from sepsis via repressing HMGB1 in an experimental model

DOI: 10.26355/eurrev_202009_23063

PMID: 33015817
Sample: Serum
FEBS LETTERS (2020) IF: 3.057
Islet‐cell autoantigen 69 accelerates liver regeneration by downregulating Tgfbr1 and attenuating Tgfβ signaling in mice

DOI: 10.1002/1873-3468.13859

Sample: Serum
PARASITOLOGY (2021) IF: 3.234
A combination of pirfenidone and TGF-β inhibition mitigates cystic echinococcosis-associated hepatic injury

DOI: 10.1017/S0031182021000287

PMID: 33583470
Sample: Serum
FOOD CHEMISTRY (2013) IF: 3.334
Dietary supplementation with purified mulberry (Morus australis Poir) anthocyanins suppresses body weight gain in high-fat diet fed C57BL/6 mice

DOI: 10.1016/j.foodchem.2013.03.046

Sample: Serum
LIFE SCIENCES (2020) IF: 3.647
Pretreatment of exosomes derived from hUCMSCs with TNF-α ameliorates acute liver failure by inhibiting the activation of NLRP3 in macrophage

DOI: 10.1016/j.lfs.2020.117401

PMID: 32035931
Sample: Whole Blood,Tissue homogenate
TOXICOLOGY LETTERS (2017) IF: 3.858
Hesperetin derivative-14 alleviates inflammation by activating PPAR-γ in mice with CCl4-induced acute liver injury and LPS-treated RAW264.7 cells

DOI: 10.1016/j.toxlet.2017.04.008

Sample: Serum,Cell culture supernatant
INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
Cardiomyocyte-specific deletion of Senp2 contributes to CVB3 viral replication and inflammation

DOI: 10.1016/j.intimp.2020.106941

PMID: 33182061
Sample: serum

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