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Mouse CRP(C-Reactive Protein) ELISA Kit

Citations(13)Uniprot : P14847

Cat.No.:E-EL-M0053
Reactivity: Mouse

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96T
48T
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96T*5
96T*10

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Properties

Format	24T, 48T, 96T, 96T5, 96T10
Assay time	3.5h
Sample type	serum, plasma and other biological fluids
Sample volume	100μL
Sample size	Run up to 46 samples in duplicate and 30 samples in triplicate
Storage	2-8℃
Interpretation	Detection range: 0.03-25 ng/mL Sensitivity: 0.02 ng/mL
Application	Quantitative determination of Human CRP concentrations in human serum, plasma and other biological fluids
Reproducibility	Both intra-CV and inter-CV are < 10%

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CRP. You can calculate the concentration of Mouse CRP in the samples by comparing the OD of the samples to the standard curve.

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

CHEMICAL ENGINEERING JOURNAL (2022) IF: 16.744
Tumor microenvironment activated nanoreactors for chemiluminescence imaging-guided simultaneous elimination of breast tumors and tumor-resident intracellular pathogens

DOI: 10.1016/j.cej.2022.139939

Sample: infectious tissues
eLife (2021) IF: 8.1460
Paternal multigenerational exposure to an obesogenic diet drives epigenetic predisposition to metabolic diseases in mice

DOI: 10.7554/eLife.61736

Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
MCC950 attenuates doxorubicin-induced myocardial injury in vivo and in vitro by inhibiting NLRP3-mediated pyroptosis

DOI: 10.1016/j.biopha.2021.112133

PMID: 34474337
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
Curcumin modulates multiple cell death, matrix metalloproteinase activation and cardiac protein release in susceptible and resistant Plasmodium berghei-infected mice

DOI: 10.1016/j.biopha.2021.112454

PMID: 34894518
Sample: serum
Frontiers in Nutrition (2022) IF: 6.59
Effects of Xinjiang wild cherry plum (Prunus divaricata Ledeb) anthocyanin-rich extract on the plasma metabolome of atherosclerotic apoE-deficient mice fed a high-fat diet

DOI: 10.3389/fnut.2022.923699

Sample: serum
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
Lian-Qu formula treats metabolic syndrome via reducing fat synthesis, insulin resistance and inflammation

DOI: 10.1016/j.jep.2022.116060

Sample: serum
INFLAMMATION (2021) IF: 4.092
Immunotherapy-on-Chip Against an Experimental Sepsis Model

DOI: 10.1007/s10753-021-01506-y

PMID: 34417666
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Endothelial progenitor cell–derived exosomes ameliorate endothelial dysfunction in a mouse model of diabetes

DOI: 10.1016/j.biopha.2020.110756

Sample: Serum
SHOCK (2018) IF: 3.005
Protection Against Lipopolysaccharide-Induced Immunosuppression by IgG and IgM

DOI: 10.1097/SHK.0000000000000937

Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
Combination of tanshinone IIA and astragaloside IV attenuate atherosclerotic plaque vulnerability in ApoE(-/-) mice by activating PI3K/AKT signaling and suppressing TRL4/NF-κB signaling

DOI: 10.1016/j.biopha.2019.109729

Sample: plasma,RAW264.7 cell
Plants-Basel (2021) IF: 3.935
The Anti-Inflammatory and the Antinociceptive Effects of Mixed Agrimonia pilosa Ledeb. and Salvia miltiorrhiza Bunge Extract

DOI: 10.3390/plants10061234

Sample: plasma
Animal Cells and Systems (2022) IF: 2.398
The mixture of Agrimonia pilosa Ledeb. and Salvia miltiorrhiza Bunge. extract produces analgesic and anti-inflammatory effects in a collagen-induced arthritis mouse model

DOI: 10.1080/19768354.2022.2106302

PMID: 36046031
Sample: ankle tissue,plasma
PLoS One (2017) IF: 2.806
Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet

DOI: 10.1371/journal.pone.0185688

Sample: Serum

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