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Mouse D-LDH(D-Lactate Dehydrogenase) ELISA Kit

Cat.No.:E-EL-M0419
Reactivity: Mouse

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96T
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T
Assay time	3.5h
Detection range	4.69-300 mIU/mL
Sensitivity	2.81 mIU/mL
Sample type &Sample volume	Serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse D-LDH in samples. No significant cross-reactivity or interference between Mouse D-LDH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse D-LDH concentrations in Serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse D-LDH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse D-LDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse D-LDH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse D-LDH. You can calculate the concentration of Mouse D-LDH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse D-LDH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse D-LDH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (mIU/mL)	16.10	35.70	136.40	16.20	38.00	141.80
Standard deviation	1.00	1.50	4.20	1.10	1.60	6.70
CV (%)	6.21	4.20	3.08	6.79	4.21	4.72

Recovery

The recovery of Mouse D-LDH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-100	91
EDTA plasma (n=8)	92-109	100
Cell culture media (n=8)	92-108	98

Target Information

Synonyms

DLD, LDHD, Probable D-lactate dehydrogenase,mitochondrial,

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
MCC950 attenuates doxorubicin-induced myocardial injury in vivo and in vitro by inhibiting NLRP3-mediated pyroptosis

DOI: 10.1016/j.biopha.2021.112133

PMID: 34474337
Sample: serum
PHYTOMEDICINE (2022) IF: 5.34
Tetrahydrocurcumin improves lipopolysaccharide-induced myocardial dysfunction by inhibiting oxidative stress and inflammation via JNK/ERK signaling pathway regulation

DOI: 10.1016/j.phymed.2022.154283

PMID: 35779282
Sample: serum
Journal of Translational Medicine (2021) IF: 5.531
microRNA-193-3p attenuates myocardial injury of mice with sepsis via STAT3/HMGB1 axis

DOI: 10.1186/s12967-021-03022-x

PMID: 34503521
Sample: serum
Cardiovascular Therapeutics (2022) IF: 3.023
L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway

DOI: 10.1155/2022/9615674

PMID: 35692375
Sample: plasma
Molecular Medicine Reports (2022) IF: 3.423
Effects of boric acid and potassium metaborate on cytokine levels and redox stress parameters in a wound model infected with methicillin‑resistant Staphylococcus aureus

DOI: 10.3892/mmr.2022.12809

Sample: L929 cell
Experimental and Therapeutic Medicine (2021) IF: 2.447
Combination of endothelial progenitor cells and BB‑94 significantly alleviates brain damage in a mouse model of diabetic ischemic stroke

DOI: 10.3892/etm.2021.10221

Sample: Cell culture supernatant
BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2019) IF: 1.917
Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice

DOI: 10.3967/bes2018.101

Sample: Plasma,Tissue homogenate

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