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Mouse DAO(Diamine Oxidase) ELISA Kit

Citations(7)Uniprot : Q8JZQ5

Cat.No.:E-EL-M0412
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	3.91-250 ng/mL
Sensitivity	2.35 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse DAO in samples. No significant cross-reactivity or interference between Mouse DAO and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse DAO concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DAO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DAO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse DAO. You can calculate the concentration of Mouse DAO in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse DAO were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse DAO were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	12.97	33.80	108.07	13.80	36.80	107.81
Standard deviation	0.66	1.84	4.82	0.93	1.56	4.12
CV (%)	5.09	5.44	4.46	6.74	4.24	3.82

Recovery

The recovery of Mouse DAO spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-102	94
EDTA plasma (n=8)	94-106	100
Cell culture media (n=8)	94-107	101

Linearity

Samples were spiked with high concentrations of Mouse DAO and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q8JZQ5
Synonyms	AOC1, ABP1, Amiloride Binding Protein 1, Amine Oxidase(Copper-Containing)
Research Area	Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

PHARMACOLOGICAL RESEARCH (2020) IF: 5.893
Cryptotanshinone alleviates chemotherapy-induced colitis in mice with colon cancer via regulating fecal-bacteria-related lipid metabolism

DOI: 10.1016/j.phrs.2020.105232

PMID: 33027716
Sample: Serum
NUTRITION (2022) IF: 4.008
Lactobacillus paracasei from Koumiss Ameliorates Diarrhea in mice via Tight Junctions Modulation

DOI: 10.1016/j.nut.2021.111584

PMID: 35395579
Sample: plasma
Frontiers in Pharmacology (2020) IF: 4.225
Combination Compositions Composed of l-Glutamine and Si-Jun-Zi-Tang Might Be a Preferable Choice for 5-Fluorouracil-Induced Intestinal Mucositis: An Exploration in a Mouse Model

DOI: 10.3389/fphar.2020.00918

PMID: 32625099
Sample: Serum
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Dihydrotanshinone attenuates chemotherapy-induced intestinal mucositis and alters fecal microbiota in mice

DOI: 10.1016/j.biopha.2020.110262

Sample: serum
INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
Atractyloside-A ameliorates spleen deficiency diarrhea by interfering with TLR4/MyD88/NF-κB signaling activation and regulating intestinal flora homeostasis

DOI: 10.1016/j.intimp.2022.108679

PMID: 35279514
Sample: serum
Toxins (2020) IF: 3.531
Betulinic Acid Ameliorates the T-2 Toxin-Triggered Intestinal Impairment in Mice by Inhibiting Inflammation and Mucosal Barrier Dysfunction through the NF-κB Signaling Pathway

DOI: 10.3390/toxins12120794

PMID: 33322178
Sample: Serum
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology (2022) IF: 2.227
Electroacupuncture modulates gut microbiota in mice: A potential target in postoperative cognitive dysfunction

DOI: 10.1002/ar.25065

PMID: 36094150
Sample: Hippocampal tissue

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