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Mouse HMGB-1(High Mobility Group Protein B1) ELISA Kit

Citations(46)Uniprot : P63158

  • Cat.No.:E-EL-M0676

  • Reactivity: Mouse
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse HMGB-1 in samples. No significant cross-reactivity or interference between Mouse HMGB-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse HMGB-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HMGB-1. You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse HMGB-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse HMGB-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 50.50 98.56 334.18 52.21 95.09 337.87
Standard deviation 2.65 4.51 17.78 3.53 5.30 13.18
CV (%) 5.25 4.58 5.32 6.76 5.57 3.90

Recovery

The recovery of Mouse HMGB-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-105 99
EDTA plasma (n=8) 88-103 94
Cell culture media (n=8) 83-98 90

Linearity

Samples were spiked with high concentrations of Mouse HMGB-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P63158
SynonymsHMGB1, HMG1, HMG3, SBP-1, Amphoterin
Research AreaCancer, Epigenetics and Nuclear Signaling

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. BIOMATERIALS (2022) IF: 15.304
    Self-amplified ROS production from fatty acid oxidation enhanced tumor immunotherapy by atorvastatin/PD-L1 siRNA lipopeptide nanoplexes

    DOI: 10.1016/j.biomaterials.2022.121902
    Sample: B16-F10 cell,CT-26 cell
  2. BIOMATERIALS (2022) IF: 15.304
    Liposome-mediated PD-L1 multivalent binding promotes the lysosomal degradation of PD-L1 for T cell-mediated antitumor immunity

    DOI: 10.1016/j.biomaterials.2022.121841

    PMID: 36206664

    		 Sample: CT26 cells
  3. ADVANCED FUNCTIONAL MATERIALS (2019) IF: 15.621
    Transformable Nanoparticle‐Enabled Synergistic Elicitation and Promotion of Immunogenic Cell Death for Triple‐Negative Breast Cancer Immunotherapy

    DOI: 10.1002/adfm.201905213
    Sample: Cell culture supernatant
  4. ACS Nano (2021) IF: 15.881
    Immunogenic Hybrid Nanovesicles of Liposomes and Tumor-Derived Nanovesicles for Cancer Immunochemotherapy

    DOI: 10.1021/acsnano.0c09681

    PMID: 33470095

    		 Sample: Cell culture supernatant
  5. Bioactive Materials (2021) IF: 14.593
    Mature dendritic cell-derived dendrosomes swallow oxaliplatin-loaded nanoparticles to boost immunogenic chemotherapy and tumor antigen-specific immunotherapy

    DOI: 10.1016/j.bioactmat.2021.12.020

    PMID: 35386340

    		 Sample: CT26 cell
  6. Nature Communications (2021) IF: 14.919
    Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer

    DOI: 10.1038/s41467-021-24564-0

    PMID: 34262026

    		 Sample: 4T1 cells
  7. BIOMATERIALS (2021) IF: 12.479
    Vanadyl nanocomplexes enhance photothermia-induced cancer immunotherapy to inhibit tumor metastasis and recurrence

    DOI: 10.1016/j.biomaterials.2021.121130

    PMID: 34534862

    		 Sample: B16F10 cell
  8. BIOMATERIALS (2021) IF: 12.479
    Polymeric PD-L1 blockade nanoparticles for cancer photothermal-immunotherapy

    DOI: 10.1016/j.biomaterials.2021.121312

    PMID: 34896861

    		 Sample: CT26 cell
  9. Small (2020) IF: 11.459
    Injectable Liquid Crystal Formation System for Reshaping Tumor Immunosuppressive Microenvironment to Boost Antitumor Immunity: Postoperative Chemoimmunotherapy

    DOI: 10.1002/smll.202004905

    PMID: 33206460

    		 Sample: Cell culture supernatant
  10. Acta Pharmaceutica Sinica B (2021) IF: 11.614
    Enhancing cancer chemo-immunotherapy by biomimetic nanogel with tumor targeting capacity and rapid drug-releasing in tumor microenvironment

    DOI: 10.1016/j.apsb.2021.11.004

    PMID: 35646526

    		 Sample: 4T1 cells
  11. PHARMACOLOGICAL RESEARCH (2023) IF: 10.334
    Liraglutide abrogates nephrotoxic effects of chemotherapies

    DOI: 10.1016/j.phrs.2023.106680
    Sample: Serum
  12. JOURNAL OF NANOBIOTECHNOLOGY (2019) IF: 10.435
    Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer

    DOI: 10.1186/s12951-019-0541-8

    PMID: 31623629

    		 Sample: Cell culture supernatant
  13. Science Immunology (2019) IF: 10.551
    ATP release drives heightened immune responses associated with hypertension

    DOI: 10.1126/sciimmunol.aau6426
    Sample: plasma
  14. ACS Applied Materials & Interfaces (2021) IF: 9.2289
    Laser/GSH-Activatable Oxaliplatin/Phthalocyanine-Based Coordination Polymer Nanoparticles Combining Chemophotodynamic Therapy to Improve Cancer Immunotherapy

    DOI: 10.1021/acsami.1c11327

    PMID: 34396771

    		 Sample: 4T1 cell
  15. ACS Applied Materials & Interfaces (2022) IF: 9.2289
    Bioactive Injectable Hydrogel Dressings for Bacteria-Infected Diabetic Wound Healing: A “Pull–Push” Approach

    DOI: 10.1021/acsami.2c04300
    Sample: regenerated wound samples
  16. JOURNAL OF CONTROLLED RELEASE (2021) IF: 9.776
    Systemic administration of polymersomal oncolytic peptide LTX-315 combining with CpG adjuvant and anti-PD-1 antibody boosts immunotherapy of melanoma

    DOI: 10.1016/j.jconrel.2021.06.032

    PMID: 34174350

    		 Sample: B16F10 cells
  17. JOURNAL OF MEDICINAL CHEMISTRY (2022) IF: 8.039
    PDTAC: Targeted Photodegradation of GPX4 Triggers Ferroptosis and Potent Antitumor Immunity

    DOI: 10.1021/acs.jmedchem.2c00855

    PMID: 36066386

    		 Sample: B16 cell
  18. OncoImmunology (2022) IF: 8.1099
    Carbon ion radiotherapy triggers immunogenic cell death and sensitizes melanoma to anti-PD-1 therapy in mice

    DOI: 10.1080/2162402X.2022.2057892

    PMID: 35355680

    		 Sample: serum
  19. LIVER INTERNATIONAL (2022) IF: 8.754
    Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury

    DOI: 10.1111/liv.15457

    PMID: 36251580

    		 Sample: serum
  20. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023) IF: 7.129
    Ferroptosis contribute to neonicotinoid imidacloprid-evoked pyroptosis by activating the HMGB1-RAGE/TLR4-NF-κB signaling pathway

    DOI: 10.1016/j.ecoenv.2023.114655
    Sample: serum
  21. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Inhibition of Netosis with PAD Inhibitor Attenuates Endotoxin Shock Induced Systemic Inflammation

    DOI: 10.3390/ijms232113264

    PMID: 36362052

    		 Sample: serum
  22. Asian Journal of Pharmaceutical Sciences (2021) IF: 6.598
    Liposomal remdesivir inhalation solution for targeted lung delivery as a novel therapeutic approach for COVID-19

    DOI: 10.1016/j.ajps.2021.09.002

    PMID: 34703490

    		 Sample: serum BALF,A549 cell
  23. Cancers (2021) IF: 6.639
    Immunogenic Cell Death by the Novel Topoisomerase I Inhibitor TLC388 Enhances the Therapeutic Efficacy of Radiotherapy

    DOI: 10.3390/cancers13061218

    PMID: 33799527

    		 Sample: Cell culture supernatant
  24. Cell Death Discovery (2016) IF: 5.241
    Retinal pigment epithelial cell necroptosis in response to sodium iodate

    DOI: 10.1038/cddiscovery.2016.54
    Sample: vitreous humor
  25. CELL PROLIFERATION (2020) IF: 5.753
    TNF‐α/HMGB1 inflammation signalling pathway regulates pyroptosis during liver failure and acute kidney injury

    DOI: 10.1111/cpr.12829
    Sample: serum
  26. Frontiers in Microbiology (2019) IF: 4.259
    Galectin-3 Deficiency Facilitates TNF-α-Dependent Hepatocyte Death and Liver Inflammation in MCMV Infection

    DOI: 10.3389/fmicb.2019.00185

    PMID: 30800112

    		 Sample: Tissue homogenate
  27. Frontiers in Physiology (2021) IF: 4.566
    Osthole Blocks HMGB1 Release From the Nucleus and Confers Protective Effects Against Renal Ischemia-Reperfusion Injury.

    DOI: 10.3389/fphys.2021.735425
    Sample: renal tissue
  28. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2019) IF: 4.658
    Cigarette smoke‐induced HMGB1 translocation and release contribute to migration and NF‐κB activation through inducing autophagy in lung macrophages

    DOI: 10.1111/jcmm.14789
    Sample: Serum,Cell culture supernatant
  29. TRANSPLANTATION (2019) IF: 4.743
    Hypothermic Oxygenated Machine Perfusion Alleviates Donation After Circulatory Death Liver Injury Through Regulating P-selectin-dependent and -independent Pathways in Mice

    DOI: 10.1097/TP.0000000000002621
    Sample: Tissue homogenate
  30. BIOORGANIC CHEMISTRY (2020) IF: 4.831
    Structure-based design of glycyrrhetinic acid derivatives as potent anti-sepsis agents targeting high-mobility group box-1

    DOI: 10.1016/j.bioorg.2020.104461
    Sample: Plasma,Cell culture supernatant
  31. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    The receptor for advanced glycation end products mediates dysfunction of airway epithelial barrier in a lipopolysaccharides-induced murine acute lung injury model

    DOI: 10.1016/j.intimp.2021.107419

    PMID: 33548580

    		 Sample: Bronchoalveolar Lavage Fluid
  32. European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
    MiR-181-5p protects mice from sepsis via repressing HMGB1 in an experimental model

    DOI: 10.26355/eurrev_202009_23063

    PMID: 33015817

    		 Sample: Serum
  33. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY (2018) IF: 3.164
    SIRT1-mediated HMGB1 deacetylation suppresses sepsis-associated acute kidney injury

    DOI: 10.1152/ajprenal.00119.2018

    PMID: 30379096

    		 Sample: Serum
  34. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2017) IF: 3.226
    High-Mobility Group Box 1 Disrupts Metabolic Function with Cigarette Smoke Exposure in a Ceramide-Dependent Manner

    DOI: 10.3390/ijms18051099
    Sample: Serum,Cell culture supernatant
  35. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2020) IF: 3.47
    Honokiol Reduces Fungal Load, Toll-Like Receptor-2, and Inflammatory Cytokines in Aspergillus fumigatus Keratitis

    DOI: 10.1167/iovs.61.4.48

    PMID: 32347916

    		 Sample: Cell culture supernatant
  36. JOURNAL OF NEUROIMMUNOLOGY (2021) IF: 3.478
    Re-exposure to alarmin HMGB1 and cytokine IL-1 beta induces differential innate immune response generation in mouse brain

    DOI: 10.1016/j.jneuroim.2021.577625

    PMID: 34153804

    		 Sample: brain
  37. NEUROSCIENCE (2021) IF: 3.59
    Hydrogen Alleviates Neuronal Injury and Neuroinflammation Induced by Microglial Activation via the Nuclear Factor Erythroid 2-related Factor 2 Pathway in Sepsis-associated Encephalopathy

    DOI: 10.1016/j.neuroscience.2021.05.003

    PMID: 33992722

    		 Sample: Cell culture supernatant,Tissue homogenate(Brain)
  38. LABORATORY INVESTIGATION (2019) IF: 3.684
    JAK2/STAT1-mediated HMGB1 translocation increases inflammation and cell death in a ventilator-induced lung injury model

    DOI: 10.1038/s41374-019-0308-8

    PMID: 31467427

    		 Sample: Cell lysate,Bronchoalveolar Lavage Fluid,Tissue ho
  39. Parasites & Vectors (2022) IF: 3.876
    Deficiency of PKCλ/ι alleviates the liver pathologic impairment of Schistosoma japonicum infection by thwarting Th2 response

    DOI: 10.1186/s13071-022-05283-x

    PMID: 35505421

    		 Sample: serum
  40. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    miR-129-5p alleviates LPS-induced acute kidney injury via targeting HMGB1/TLRs/NF-kappaB pathway

    DOI: 10.1016/j.intimp.2020.107016

    PMID: 33039954

    		 Sample: Serum
  41. INTERNATIONAL IMMUNOPHARMACOLOGY (2015) IF: 2.472
    Hesperidin ameliorates lipopolysaccharide-induced acute lung injury in mice by inhibiting HMGB1 release

    DOI: 10.1016/j.intimp.2015.02.022
    Sample: Bronchoalveolar Lavage Fluid
  42. INTERNATIONAL IMMUNOPHARMACOLOGY (2014) IF: 2.711
    Protective effects of hesperidin on concanavalin A-induced hepatic injury in mice

    DOI: 10.1016/j.intimp.2014.05.018
    Sample: Serum
  43. INFLAMMATION (2018) IF: 2.884
    the Nrf2-ARE Signaling Pathway

    DOI: 10.1007/s10753-018-0915-3
    Sample: Tissue homogenate
  44. International Journal of Ophthalmology (2020) IF: 1.33
    Anti-inflammatory effects of astaxanthin against fungal keratitis

    DOI: 10.18240/ijo.2020.11.01

    PMID: 33214996

    		 Sample: Tissue homogenate(Corneas)
  45. MEDICAL SCIENCE MONITOR (2016) IF: 1.405
    Protective Role of Grape Seed Proanthocyanidins Against Ccl4 Induced Acute Liver Injury in Mice

    DOI: 10.12659/MSM.895552
    Sample: Serum
  46. Heliyon (2021)
    High mobility group box-1 (HMGB-1) and its receptors in the pathogenesis of malaria-associated acute lung injury/acute respiratory distress syndrome in a mouse model

    DOI: 10.1016/j.heliyon.2021.e08589
    Sample: lung tissue,plasma
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